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Perform Volcano plot analysis on selected experimental conditions followed by MS search on significant features
Group1
Experimental factor
Group2
Time:15 | Protein:Active (3)
Time:15 | Protein:Inactivated (3)
Time:30 | Protein:Active (3)
Time:30 | Protein:Inactivated (3)
Time:- | Protein:Active (3)
Time:- | Protein:Inactivated (3)
Filtering Parameters
Select threshold % for selection below:
100% (option 4 only)
95%
90%
85%
80%
75%
70%
65%
60%
55%
50%
45%
40%
35%
30%
20%
10%
0%
Option 1:
No filtering
Option 2:
Filter by Sum
(Analytes whose sum for all samples falls below the threshold % will be excluded)
Option 3:
Filter by Mean
(Analytes whose mean for all samples falls below the threshold % will be excluded)
Option 4:
Filter by Median
(Analytes whose median for all samples falls below the threshold % will be excluded)
Option 5:
Filter by Sample threshold
At least one of the 2 selected groups
Both groups
must exceed the above threshold per analyte
In order for an analyte to be retained, the % of samples in one/both groups which are non-zero must exceed the threshold.
Option 6:
Remove all analytes containing null values
Sample Normalization Parameters
Normalize samples by:
None
Sample means
Sample sums
Volcano Plot Parameters
P-value cutoff:
0.1
0.05
0.02
0.01
0.005
0.002
0.001
0.0005
0.0002
Fold-change cutoff:
1.1
1.2
1.25
1.33
1.5
2
3
4
5
MS Search Parameters
Search Refmet database
MS Ion polarity:POSITIVE
MS search m/z tolerance:
0.1
0.05
0.02
0.01
0.005
0.002
0.001
0.0005
0.0002
Feature order:
m/z_rt
rt_m/z
Ion adducts(choose at least one)
Positive mode:
[M+H]+
[M+H-H2O]+
[M+Na]+
[M+NH4]+
[M+K]+
[M+2H]2+
[M+2Na]2+
[M+2Na-H]+
[M+H-EtnP]+
[M+H-SerP]+
Metabolomics Workbench
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