#METABOLOMICS WORKBENCH jgiafaglione_20230912_161426 DATATRACK_ID:4311 STUDY_ID:ST002860 ANALYSIS_ID:AN004690 PROJECT_ID:PR001788 VERSION 1 CREATED_ON September 13, 2023, 3:21 pm #PROJECT PR:PROJECT_TITLE MYC is a regulator of AR inhibition-induced metabolic requirements in prostate PR:PROJECT_TITLE cancer PR:PROJECT_SUMMARY Advanced prostate cancers are treated with therapies targeting the androgen PR:PROJECT_SUMMARY receptor (AR) signaling pathway. While many tumors initially respond to AR PR:PROJECT_SUMMARY inhibition, nearly all develop resistance. It is critical to understand how PR:PROJECT_SUMMARY prostate tumor cells respond to AR inhibition in order to exploit PR:PROJECT_SUMMARY therapy-induced phenotypes prior to the outgrowth of treatment-resistant PR:PROJECT_SUMMARY disease. Here, we comprehensively characterize the effect of AR blockade on PR:PROJECT_SUMMARY prostate cancer metabolism using transcriptomics, metabolomics and bioenergetics PR:PROJECT_SUMMARY approaches. The metabolic response to AR inhibition is defined by reduced PR:PROJECT_SUMMARY glycolysis, robust elongation of mitochondria, and increased reliance on PR:PROJECT_SUMMARY mitochondrial oxidative metabolism. We establish DRP1 activity and MYC signaling PR:PROJECT_SUMMARY as mediators of AR blockade-induced metabolic phenotypes. Rescuing DRP1 PR:PROJECT_SUMMARY phosphorylation after AR inhibition restores mitochondrial fission, while PR:PROJECT_SUMMARY rescuing MYC restores glycolytic activity and prevents sensitivity to complex I PR:PROJECT_SUMMARY inhibition. Our study provides new insight into the regulation of PR:PROJECT_SUMMARY treatment-induced metabolic phenotypes and vulnerabilities in prostate cancer. PR:INSTITUTE UCLA PR:LABORATORY Goldstein Lab PR:LAST_NAME Giafaglione PR:FIRST_NAME Jenna PR:ADDRESS 610 Charles E Young Dr S, Los Angeles, CA, 90024, USA PR:EMAIL jgiafaglione@g.ucla.edu PR:PHONE 408-728-3065 #STUDY ST:STUDY_TITLE Glucose tracing in vehicle- and Enzalutamide-treated 16D prostate cancer cells ST:STUDY_TITLE (replicates 2 and 3) ST:STUDY_SUMMARY In this study, we sought to determine how Enzalutamide treatment alters ST:STUDY_SUMMARY metabolism in 16D prostate cancer cells (replicates 2 and 3). ST:INSTITUTE UCLA ST:LABORATORY Goldstein Lab ST:LAST_NAME Giafaglione ST:FIRST_NAME Jenna ST:ADDRESS 610 Charles E Young Dr S, Los Angeles, CA, 90024, USA ST:EMAIL jgiafaglione@g.ucla.edu ST:PHONE 408-728-3065 #SUBJECT SU:SUBJECT_TYPE Cultured cells SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - 20201210_Naive-1 Treatment:Vehicle (DMSO) RAW_FILE_NAME=20201210_Naive-1.D SUBJECT_SAMPLE_FACTORS - 20201210_Naive-2 Treatment:Vehicle (DMSO) RAW_FILE_NAME=20201210_Naive-2.D SUBJECT_SAMPLE_FACTORS - 20201210_Naive-3 Treatment:Vehicle (DMSO) RAW_FILE_NAME=20201210_Naive-3.D SUBJECT_SAMPLE_FACTORS - 20201210_LTEnza-1 Treatment:10uM Enzalutamide RAW_FILE_NAME=20201210_LTEnza-1.D SUBJECT_SAMPLE_FACTORS - 20201210_LTEnza-2 Treatment:10uM Enzalutamide RAW_FILE_NAME=20201210_LTEnza-2.D SUBJECT_SAMPLE_FACTORS - 20201210_LTEnza-3 Treatment:10uM Enzalutamide RAW_FILE_NAME=20201210_LTEnza-3.D SUBJECT_SAMPLE_FACTORS - 20201211_Naive-1 Treatment:Vehicle (DMSO) RAW_FILE_NAME=20201211_Naive-1.D SUBJECT_SAMPLE_FACTORS - 20201211_Naive-2 Treatment:Vehicle (DMSO) RAW_FILE_NAME=20201211_Naive-2.D SUBJECT_SAMPLE_FACTORS - 20201211_Naive-3 Treatment:Vehicle (DMSO) RAW_FILE_NAME=20201211_Naive-3.D SUBJECT_SAMPLE_FACTORS - 20201211_LTEnza-1 Treatment:10uM Enzalutamide RAW_FILE_NAME=20201211_LTEnza-1.D SUBJECT_SAMPLE_FACTORS - 20201211_LTEnza-2 Treatment:10uM Enzalutamide RAW_FILE_NAME=20201211_LTEnza-2.D SUBJECT_SAMPLE_FACTORS - 20201211_LTEnza-3 Treatment:10uM Enzalutamide RAW_FILE_NAME=20201211_LTEnza-3.D #COLLECTION CO:COLLECTION_SUMMARY 16D prostate cancer cells were cultured with vehicle or Enzalutamide for >2 CO:COLLECTION_SUMMARY months. CO:SAMPLE_TYPE Cultured cells #TREATMENT TR:TREATMENT_SUMMARY Vehicle- and Enzalutamide-treated 16D cells were cultured in U13C-glucose TR:TREATMENT_SUMMARY containing media for 24 hours. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Cell plates were placed on ice and quickly washed with ice-cold 0.9% (w/v) NaCl. SP:SAMPLEPREP_SUMMARY Cells were immediately treated with 500 μL of ice-cold MeOH and 200 μL water SP:SAMPLEPREP_SUMMARY containing 1 μg of the internal standard norvaline. Cells were then scraped and SP:SAMPLEPREP_SUMMARY placed in 1.5 mL centrifuge tubes kept on ice. Next, 500 μL of chloroform was SP:SAMPLEPREP_SUMMARY added, after which samples were vortexed for 1 minute and then spun at 10,000g SP:SAMPLEPREP_SUMMARY for 5 minutes at 4C. The aqueous layer was transferred to a GC/MS sample vial SP:SAMPLEPREP_SUMMARY and dried overnight using a refrigerated CentriVap. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE GC CH:INSTRUMENT_NAME Agilent 7890A CH:COLUMN_NAME Agilent DB5-MS (30m x 0.25mm, 0.25um) CH:SOLVENT_A N/A (GC-MS) CH:SOLVENT_B N/A (GC-MS) CH:FLOW_GRADIENT N/A (GC-MS) CH:FLOW_RATE 1 ml/min CH:COLUMN_TEMPERATURE 320 #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Agilent 5977B MS:INSTRUMENT_TYPE GC-TOF MS:MS_TYPE EI MS:ION_MODE POSITIVE MS:MS_COMMENTS Data was analyzed using Agilent MassHunter software. Stable isotope tracing data MS:MS_COMMENTS was corrected for natural abundance of heavy isotopes with FluxFix software MS:MS_COMMENTS using a reference set of unlabeled metabolite standards2. #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS abundance MS_METABOLITE_DATA_START Samples 20201210_LTEnza-1 20201210_LTEnza-2 20201210_LTEnza-3 20201210_Naive-1 20201210_Naive-2 20201210_Naive-3 20201211_LTEnza-1 20201211_LTEnza-2 20201211_LTEnza-3 20201211_Naive-1 20201211_Naive-2 20201211_Naive-3 Factors Treatment:10uM Enzalutamide Treatment:10uM Enzalutamide Treatment:10uM Enzalutamide Treatment:Vehicle (DMSO) Treatment:Vehicle (DMSO) Treatment:Vehicle (DMSO) Treatment:10uM Enzalutamide Treatment:10uM Enzalutamide Treatment:10uM Enzalutamide Treatment:Vehicle (DMSO) Treatment:Vehicle (DMSO) Treatment:Vehicle (DMSO) a-Ketoglutarate(M0) 2308 3174 2179 3575 3563 4523 7286 5341 5808 6614 5828 9048 a-Ketoglutarate(M1) 824 1137 796 1090 1091 1394 2529 1845 2018 1974 1747 2699 a-Ketoglutarate(M2) 1081 1511 1045 1442 1467 1844 3087 2227 2450 2376 2110 3301 a-Ketoglutarate(M3) 465 630 447 561 584 719 1180 847 925 828 743 1160 a-Ketoglutarate(M4) 374 500 278 483 550 664 48 30 33 583 543 865 a-Ketoglutarate(M5) 137 188 160 206 223 272 267 170 202 214 198 320 a-Ketoglutarate(M6) 94 125 151 47 144 167 46 44 47 56 39 67 Alanine260(M0) 113884 137830 109041 191358 180799 184148 145472 116239 128076 165788 145905 211726 Alanine260(M1) 28584 34508 27286 51066 47917 48931 35676 28583 31482 43107 37802 54995 Alanine260(M2) 17619 21294 16795 39668 36645 37751 20870 16717 18414 30936 27085 39785 Alanine260(M3) 105997 127378 100693 351210 317043 330804 117047 94222 101646 250370 218903 325494 Alanine260(M4) 20851 25144 19774 69836 62976 65796 23077 18580 20011 49665 43333 64748 Asparagine(M0) 209041 206280 165866 399453 321530 350868 205344 140309 171345 251834 252799 305606 Asparagine(M1) 78905 77957 62473 149738 119985 131481 76785 52343 64050 93399 94073 114070 Asparagine(M2) 42024 41477 33260 80295 64303 70551 40110 27205 33334 49235 49666 60235 Asparagine(M3) 12947 12776 10263 25560 20481 22395 11786 7962 9758 14844 14994 18277 Asparagine(M4) 4052 4013 3231 8265 6596 7247 3539 2392 2929 4576 4626 5677 Asparagine(M5) 1069 1037 850 2227 1744 1935 872 583 724 1171 1184 1459 Aspartate418(M0) 61459 65072 53219 121351 112107 115674 73460 50279 60418 113736 113876 147236 Aspartate418(M1) 28200 29754 24521 51618 47813 49216 32528 22245 27041 46674 46913 60897 Aspartate418(M2) 23275 24393 20216 48224 44770 45858 25738 17626 21538 39690 39997 52319 Aspartate418(M3) 11883 12389 10286 28804 26688 27148 11939 8249 10039 20124 20248 26650 Aspartate418(M4) 4994 5186 4340 13161 12162 12348 4711 3232 3957 8291 8306 11021 Aspartate418(M5) 1547 1604 1343 4340 4004 4046 1435 982 1183 2599 2596 3451 Citrate459(M0) 90935 95835 76428 165000 144693 147577 113527 78309 102254 111678 120563 143889 Citrate459(M1) 44368 46729 37427 75338 66328 67592 53987 37071 48652 49841 53882 64331 Citrate459(M2) 61434 64676 51711 148342 131127 134585 72641 49981 65130 93168 100179 120266 Citrate459(M3) 30621 32102 25690 71547 63458 64931 34029 23349 30303 41841 45230 54307 Citrate459(M4) 19556 20575 16391 54483 48110 49307 20731 14216 18360 29443 31873 38495 Citrate459(M5) 9033 9438 7564 29407 26027 26640 8862 6122 7835 14149 15255 18566 Citrate459(M6) 3433 3563 2853 12337 10821 11217 3237 2235 2836 5635 6083 7376 Citrate459(M7) 1085 1110 896 4102 3599 3722 997 678 868 1787 1929 2355 Citrate591(M0) 16371 17233 13728 29518 25955 26358 20399 13987 18370 20021 21614 25963 Citrate591(M1) 9925 10483 8319 16984 14988 15235 12086 8338 10837 11331 12213 14716 Citrate591(M2) 12459 13139 10466 28864 25519 26082 14765 10157 13222 18202 19638 23713 Citrate591(M3) 7059 7413 5907 16267 14472 14828 7987 5457 7101 9754 10538 12695 Citrate591(M4) 4493 4737 3776 11915 10582 10867 4867 3313 4302 6661 7189 8691 Citrate591(M5) 2209 2312 1855 6777 5949 6150 2250 1537 1972 3374 3669 4467 Citrate591(M6) 918 963 766 3103 2729 2831 893 604 795 1464 1585 1947 Citrate591(M7) 326 336 269 1177 1028 1078 308 207 265 525 573 714 Cysteine(M0) 1416 11567 9355 11026 6307 9497 29784 20682 18672 8075 10425 2200 Cysteine(M1) 477 4016 3265 3817 2178 3304 10414 7228 6491 2797 3607 756 Cysteine(M2) 291 2374 1926 2256 1278 1930 6129 4239 3818 1655 2141 451 Cysteine(M3) 42 601 487 589 333 506 1564 1090 980 430 553 121 Cysteine(M4) 20 180 146 178 102 153 465 326 297 122 168 39 Fumarate(M0) 16148 15515 13751 41621 35368 39858 22090 16714 19903 52500 46466 68316 Fumarate(M1) 5667 5526 4916 12762 10954 12314 7324 5549 6603 15611 13820 20386 Fumarate(M2) 5332 5164 4660 13269 11507 12881 6386 4814 5787 14764 13027 19420 Fumarate(M3) 2518 2454 2195 7291 6303 7158 2747 2085 2480 6943 6109 9185 Fumarate(M4) 1108 1095 993 3097 2727 2997 1297 1045 1171 2874 2483 3722 Fumarate(M5) 272 269 246 803 708 789 317 248 284 721 629 955 GABA(M0) 114 178 112 4768 4867 5071 162 140 2908 4653 4237 6859 GABA(M1) 41 36 35 63 77 66 57 88 56 90 95 141 GABA(M2) 23 84 89 34 31 36 83 104 97 88 71 119 GABA(M3) 33 794 408 78 722 623 45 321 628 38 46 76 GABA(M4) 8 141 72 8 142 124 8 15 117 94 83 20 GABA(M5) 39 58 29 4 74 8 38 25 5 41 58 6 Glutamate432(M0) 659464 645645 486162 688637 675416 647641 668050 524064 599806 647938 652747 669195 Glutamate432(M1) 314068 307933 267740 319255 282244 299333 349022 266661 313172 263963 240197 314644 Glutamate432(M2) 374888 369223 320019 373483 333133 351217 392753 299018 350861 284132 288579 343239 Glutamate432(M3) 188841 186495 161012 174085 156084 164167 180972 136805 160405 119647 121824 145731 Glutamate432(M4) 113922 112280 97034 115849 103505 109110 101338 75862 88973 70075 71679 86695 Glutamate432(M5) 48178 47356 40894 54942 48804 51390 38550 28763 33606 28179 29087 35281 Glutamate432(M6) 15627 15405 13248 18371 16314 17168 12207 9097 10604 9094 9421 11484 Isoleu-274(M0) 19588 23174 20229 24957 28493 27271 26314 20206 21296 24618 22056 33435 Isoleucine274(M0) 20420 23995 20962 30002 31825 32585 29203 22297 23668 28998 27234 38465 Isoleucine274(M1) 5282 6156 5336 7631 8303 8320 7439 5688 6052 7374 6960 9797 Isoleucine274(M2) 2008 2396 2034 2798 291 3108 2871 2231 2156 2913 2482 3874 Isoleucine274(M3) 342 404 336 41 54 54 477 360 393 486 26 53 Isoleucine274(M4) 51 70 59 74 84 22 77 52 65 83 79 19 Isoleucine274(M5) 1 9 2 1 6 0 9 1 1 5 0 15 Isoleucine274(M6) 5 4 0 1 2 1 2 2 0 3 1 2 Isoleucine274(M7) 1 5 2 10 10 48 42 45 33 3 46 Itaconate(M0) 260 250 193 497 331 279 308 305 318 266 257 323 Itaconate(M1) 111 114 83 177 138 132 136 110 118 96 103 115 Itaconate(M2) 70 92 58 87 92 93 74 55 59 61 59 85 Itaconate(M3) 33 36 23 42 47 52 31 32 30 32 30 43 Itaconate(M4) 20331 25118 21772 26194 37654 29417 24898 29350 23644 22660 21320 30049 Itaconate(M5) 5294 6512 5905 6799 9131 6983 6409 7615 5697 6008 5474 7769 Itaconate(M6) 1475 1819 1644 1888 2672 2095 1766 2097 1691 1606 1512 1950 KIC(M0) 3555 3553 3134 4644 4193 4335 13349 10388 8840 6599 5820 8671 KIV(M0) 1073 1098 1329 1469 1355 945 1585 1377 1901 2018 1475 1698 KMV(M0) 2345 2797 2483 4202 4150 3995 6143 5340 4522 5522 4964 7669 Lactate261(M0) 19660 22791 16413 69197 50655 53722 82474 58064 55351 108220 85916 135335 Lactate261(M1) 4762 5577 4014 17611 12831 13808 19419 13697 13121 26849 21562 34009 Lactate261(M2) 3154 3734 133 14563 10164 11690 11873 8549 8206 20895 17187 27441 Lactate261(M3) 17816 22443 15665 131360 89789 109355 66040 51054 49261 182911 154995 249886 Lactate262(M4) 3464 4332 3039 25307 17384 21114 12747 9824 9540 35315 29915 48465 Malate(M0) 22152 24814 20678 77832 69610 75864 35739 24980 31533 92522 88196 120255 Malate(M1) 10602 11927 9933 32518 29362 31794 15994 11114 14119 37352 35728 48756 Malate(M2) 10078 11352 9509 31834 28930 31140 13984 9674 12402 33177 31785 43558 Malate(M3) 5285 6031 5036 18721 17016 18408 6748 4675 5948 16784 16069 22196 Malate(M4) 2404 2749 2291 8727 7899 8548 2789 1937 2478 7033 6748 9361 Malate(M5) 765 889 732 2892 2625 2843 866 595 761 2246 2131 2993 Methionine320(M0) 7408 8677 7045 7669 7738 8073 10632 7449 8617 7897 7316 10492 Methionine320(M1) 1949 2289 1847 2082 2088 2200 2795 1944 2252 2120 1960 2819 Methionine320(M2) 1094 1280 1037 1145 1153 1209 1553 1089 1267 1171 1094 1558 Methionine320(M3) 226 269 218 254 262 278 316 221 261 249 232 342 Methionine320(M4) 9 192 147 299 300 308 184 130 158 245 230 334 Methionine320(M5) 26 27 22 60 50 63 24 1 21 39 39 63 Methionine320(M6) 12 10 10 23 27 23 8 10 10 17 18 28 N-AcetylAspartate(M0) 241 5 55 378 211 264 305 224 623 N-AcetylAspartate(M1) 11 26 32 35 22 174 284 380 128 197 117 N-AcetylAspartate(M2) 1 4 5 33 173 5 34 6 15 N-AcetylAspartate(M3) 14 4 21 16 7 8 4 30 37 N-AcetylAspartate(M4) 1 19 N-AcetylAspartate(M5) 11 5 16 1 2 Norvaline 260(M0) 381672 439006 379407 327490 356681 369672 471851 404007 446796 344293 310949 437694 Norvaline 260(M1) 94409 108750 93844 80808 88381 91602 116983 100016 110773 85002 76774 108624 Norvaline 260(M2) 37121 42949 36937 31665 34979 36000 45978 39304 43617 33374 30204 42760 Norvaline 260(M3) 5814 6636 5665 4851 5414 5600 7113 6140 6872 5266 4743 6656 Norvaline 288(M0) 313437 377939 327524 267098 297664 307973 411205 351224 390192 299753 272331 385154 Norvaline 288(M1) 78835 95474 82470 67123 75089 77865 104102 88677 98640 75392 68819 97373 Norvaline 288(M2) 31225 37803 32698 26588 29386 30774 41398 35203 39211 29854 27126 38661 Norvaline 288(M3) 5085 6122 5317 4321 4827 5014 6747 5712 6369 4868 4388 6287 Ornithine474(M0) 38 39 35 32 34 27 36 41 40 24 23 22 Ornithine474(M1) 124 132 106 87 105 94 102 123 117 64 72 71 Ornithine474(M2) Ornithine474(M3) 42 48 37 30 31 31 112 103 105 46 19 29 Ornithine474(M4) 15 16 11 9 10 11 46 39 41 14 15 15 Ornithine474(M5) 26 39 28 3 4 0 43 33 44 4 6 5 Ornithine474(M6) 23 25 18 0 1 1 29 21 27 1 1 Pyruvate(M0) 43766 51395 41045 67916 60620 60688 152241 126894 126358 138254 123593 170754 Pyruvate(M1) 5677 7098 7652 11863 9929 11880 22220 18786 15988 20836 19260 26264 Pyruvate(M2) 3969 5957 4075 9982 8747 9200 11899 9999 9666 15085 13441 18940 Pyruvate(M3) 29515 34779 25826 107367 85698 96422 74244 63609 57697 140972 128106 180651 Pyruvate(M4) 2749 3337 2349 9896 7691 8718 6594 5762 5196 12960 11378 16492 Serine390(M0) 220807 238713 198253 228371 203461 209675 248419 187869 219157 187877 174560 229288 Serine390(M1) 116884 126116 104842 163624 145968 149885 120477 90967 106465 121050 112603 148804 Serine390(M2) 72564 78342 65229 113103 100919 103721 73104 55063 64562 81214 75653 100136 Serine390(M3) 47484 50919 42366 154233 136573 140032 46888 35419 41243 102446 95720 126755 Serine390(M4) 14814 15966 13285 49081 43416 44623 14568 10959 12841 32436 30255 40314 Succinate(M0) 5726 6357 4871 12887 10822 10178 23855 17966 20058 24574 20733 29863 Succinate(M1) 1723 2049 1603 3536 3029 2902 5924 4466 5115 6308 5218 7813 Succinate(M2) 1544 1934 1518 3466 3106 3027 3320 2457 2952 4711 4018 6167 Succinate(M3) 611 780 626 1404 1281 1278 872 619 756 1474 1276 2025 Succinate(M4) 378 528 420 760 746 746 479 397 422 718 648 1016 Succinate(M5) 81 113 91 171 162 163 94 74 84 158 138 221 Threonine404(M0) 15458 14609 11967 28791 21596 25297 19004 11604 16105 19169 21183 27723 Threonine404(M1) 5437 5124 4194 10104 7552 8865 6642 4073 5642 6729 7399 9713 Threonine404(M2) 2610 2472 2019 4787 3612 4214 775 1967 2701 3823 4181 5389 Threonine404(M3) 601 581 476 1105 833 983 326 472 640 960 1035 1340 Threonine404(M4) 149 139 118 268 203 233 167 117 148 103 94 121 Threonine404(M5) 28 26 20 46 3 44 42 22 22 25 22 28 Urea(M0) 136939 168699 126708 215778 208457 216026 191526 141668 149097 194061 170334 266369 Urea(M1) 28314 35357 26686 44997 43163 45181 39870 29520 31039 40562 35515 55373 Urea(M2) Valine288(M0) 7465 9227 7686 8918 9112 9361 12276 8992 9366 9593 8853 13509 Valine288(M1) 1920 2369 1967 2275 2315 2380 3177 2308 2423 2448 2262 3445 Valine288(M2) 754 918 760 887 892 919 1231 900 944 967 883 1355 Valine288(M3) 123 153 129 148 144 155 208 142 152 163 148 229 Valine288(M4) 1 26 20 22 27 25 37 24 27 24 22 34 Valine288(M5) 10 1 1 2 1 10 1 9 10 2 2 1 Valine288(M6) 0 0 0 2 2 1 1 1 2 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name a-Ketoglutarate(M0) a-Ketoglutarate(M1) a-Ketoglutarate(M2) a-Ketoglutarate(M3) a-Ketoglutarate(M4) a-Ketoglutarate(M5) a-Ketoglutarate(M6) Alanine260(M0) Alanine260(M1) Alanine260(M2) Alanine260(M3) Alanine260(M4) Asparagine(M0) Asparagine(M1) Asparagine(M2) Asparagine(M3) Asparagine(M4) Asparagine(M5) Aspartate418(M0) Aspartate418(M1) Aspartate418(M2) Aspartate418(M3) Aspartate418(M4) Aspartate418(M5) Citrate459(M0) Citrate459(M1) Citrate459(M2) Citrate459(M3) Citrate459(M4) Citrate459(M5) Citrate459(M6) Citrate459(M7) Citrate591(M0) Citrate591(M1) Citrate591(M2) Citrate591(M3) Citrate591(M4) Citrate591(M5) Citrate591(M6) Citrate591(M7) Cysteine(M0) Cysteine(M1) Cysteine(M2) Cysteine(M3) Cysteine(M4) Fumarate(M0) Fumarate(M1) Fumarate(M2) Fumarate(M3) Fumarate(M4) Fumarate(M5) GABA(M0) GABA(M1) GABA(M2) GABA(M3) GABA(M4) GABA(M5) Glutamate432(M0) Glutamate432(M1) Glutamate432(M2) Glutamate432(M3) Glutamate432(M4) Glutamate432(M5) Glutamate432(M6) Isoleu-274(M0) Isoleucine274(M0) Isoleucine274(M1) Isoleucine274(M2) Isoleucine274(M3) Isoleucine274(M4) Isoleucine274(M5) Isoleucine274(M6) Isoleucine274(M7) Itaconate(M0) Itaconate(M1) Itaconate(M2) Itaconate(M3) Itaconate(M4) Itaconate(M5) Itaconate(M6) KIC(M0) KIV(M0) KMV(M0) Lactate261(M0) Lactate261(M1) Lactate261(M2) Lactate261(M3) Lactate262(M4) Malate(M0) Malate(M1) Malate(M2) Malate(M3) Malate(M4) Malate(M5) Methionine320(M0) Methionine320(M1) Methionine320(M2) Methionine320(M3) Methionine320(M4) Methionine320(M5) Methionine320(M6) N-AcetylAspartate(M0) N-AcetylAspartate(M1) N-AcetylAspartate(M2) N-AcetylAspartate(M3) N-AcetylAspartate(M4) N-AcetylAspartate(M5) Norvaline 260(M0) Norvaline 260(M1) Norvaline 260(M2) Norvaline 260(M3) Norvaline 288(M0) Norvaline 288(M1) Norvaline 288(M2) Norvaline 288(M3) Ornithine474(M0) Ornithine474(M1) Ornithine474(M2) Ornithine474(M3) Ornithine474(M4) Ornithine474(M5) Ornithine474(M6) Pyruvate(M0) Pyruvate(M1) Pyruvate(M2) Pyruvate(M3) Pyruvate(M4) Serine390(M0) Serine390(M1) Serine390(M2) Serine390(M3) Serine390(M4) Succinate(M0) Succinate(M1) Succinate(M2) Succinate(M3) Succinate(M4) Succinate(M5) Threonine404(M0) Threonine404(M1) Threonine404(M2) Threonine404(M3) Threonine404(M4) Threonine404(M5) Urea(M0) Urea(M1) Urea(M2) Valine288(M0) Valine288(M1) Valine288(M2) Valine288(M3) Valine288(M4) Valine288(M5) Valine288(M6) METABOLITES_END #END