#METABOLOMICS WORKBENCH Marjo_20230811_20230810_211521 DATATRACK_ID:4218 STUDY_ID:ST002826 ANALYSIS_ID:AN004612 PROJECT_ID:PR001768 VERSION 1 CREATED_ON August 22, 2023, 10:59 pm #PROJECT PR:PROJECT_TITLE Cold-stimulated brown adipose tissue activation is related to changes in serum PR:PROJECT_TITLE metabolites relevant to NAD+ metabolism in humans PR:PROJECT_SUMMARY Cold-induced brown adipose tissue (BAT) activation is considered to improve PR:PROJECT_SUMMARY metabolic health. In murine BAT, cold increases the fundamental molecule for PR:PROJECT_SUMMARY mitochondrial function, nicotinamide adenine dinucleotide (NAD+), but limited PR:PROJECT_SUMMARY knowledge on NAD+ metabolism during cold in human BAT metabolism exists. We show PR:PROJECT_SUMMARY that cold increases the serum metabolites of the NAD+ salvage pathway PR:PROJECT_SUMMARY (nicotinamide and 1-methylnicotinamide) in humans. Additionally, individuals PR:PROJECT_SUMMARY with coldstimulated BAT activation have decreased levels of metabolites from the PR:PROJECT_SUMMARY de novo NAD+ biosynthesis pathway (tryptophan, kynurenine). Serum nicotinamide PR:PROJECT_SUMMARY correlates positively with cold-stimulated BAT activation, whereas tryptophan PR:PROJECT_SUMMARY and kynurenine correlates negatively. Furthermore, the expression of genes PR:PROJECT_SUMMARY involved in NAD+ biosynthesis in BAT are related to markers of metabolic health. PR:PROJECT_SUMMARY Our data indicate that cold increases serum tryptophan conversion to PR:PROJECT_SUMMARY nicotinamide to be further utilized by BAT. We conclude that NAD+ metabolism is PR:PROJECT_SUMMARY activated upon cold in humans and probably regulated in a coordinated fashion by PR:PROJECT_SUMMARY several tissues. PR:INSTITUTE University of Turku PR:LAST_NAME Virtanen PR:FIRST_NAME Kirsi A PR:ADDRESS Turku PET Centre, Turku University Hospital, Turku, Finland PR:EMAIL kirsi.virtanen@utu.fi PR:PHONE +358-040-7626564 #STUDY ST:STUDY_TITLE Cold-stimulated brown adipose tissue activation is related to changes in serum ST:STUDY_TITLE metabolites relevant to NAD+ metabolism in humans ST:STUDY_SUMMARY Cold-induced brown adipose tissue (BAT) activation is considered to improve ST:STUDY_SUMMARY metabolic health. In murine BAT, cold increases the fundamental molecule for ST:STUDY_SUMMARY mitochondrial function, nicotinamide adenine dinucleotide (NAD+), but limited ST:STUDY_SUMMARY knowledge on NAD+ metabolism during cold in human BAT metabolism exists. We show ST:STUDY_SUMMARY that cold increases the serum metabolites of the NAD+ salvage pathway ST:STUDY_SUMMARY (nicotinamide and 1-methylnicotinamide) in humans. Additionally, individuals ST:STUDY_SUMMARY with coldstimulated BAT activation have decreased levels of metabolites from the ST:STUDY_SUMMARY de novo NAD+ biosynthesis pathway (tryptophan, kynurenine). Serum nicotinamide ST:STUDY_SUMMARY correlates positively with cold-stimulated BAT activation, whereas tryptophan ST:STUDY_SUMMARY and kynurenine correlates negatively. Furthermore, the expression of genes ST:STUDY_SUMMARY involved in NAD+ biosynthesis in BAT are related to markers of metabolic health. ST:STUDY_SUMMARY Our data indicate that cold increases serum tryptophan conversion to ST:STUDY_SUMMARY nicotinamide to be further utilized by BAT. We conclude that NAD+ metabolism is ST:STUDY_SUMMARY activated upon cold in humans and probably regulated in a coordinated fashion by ST:STUDY_SUMMARY several tissues. ST:INSTITUTE University of Turku ST:LAST_NAME Virtanen ST:FIRST_NAME Kirsi ST:ADDRESS Turku PET Centre, Turku University Hospital, Turku, Finland ST:EMAIL kirsi.virtanen@utu.fi ST:PHONE +358-40-7626564 #SUBJECT SU:SUBJECT_TYPE Human SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 #FACTORS #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - QC1 Treatment:QC RAW_FILE_NAME=190201MR_PET_Hilic_neg_010; RAW_FILE_NAME=190128MR_PET_RP_pos_010; RAW_FILE_NAME=190122MR_PET_RP_neg_016; RAW_FILE_NAME=190130MR_PET_Hilic_pos_010 SUBJECT_SAMPLE_FACTORS - QC autoMSMS 10V_1 Treatment:QC RAW_FILE_NAME=190201MR_PET_Hilic_neg_011; RAW_FILE_NAME=190128MR_PET_RP_pos_011; RAW_FILE_NAME=190122MR_PET_RP_neg_017; RAW_FILE_NAME=190130MR_PET_Hilic_pos_011 SUBJECT_SAMPLE_FACTORS - QC autoMSMS 20V_1 Treatment:QC RAW_FILE_NAME=190201MR_PET_Hilic_neg_012; RAW_FILE_NAME=190128MR_PET_RP_pos_012; RAW_FILE_NAME=190122MR_PET_RP_neg_018; RAW_FILE_NAME=190130MR_PET_Hilic_pos_012 SUBJECT_SAMPLE_FACTORS - QC autoMSMS 40V_1 Treatment:QC RAW_FILE_NAME=190201MR_PET_Hilic_neg_013; RAW_FILE_NAME=190128MR_PET_RP_pos_013; RAW_FILE_NAME=190122MR_PET_RP_neg_019; RAW_FILE_NAME=190130MR_PET_Hilic_pos_013 SUBJECT_SAMPLE_FACTORS - QC2 Treatment:QC RAW_FILE_NAME=190201MR_PET_Hilic_neg_014; RAW_FILE_NAME=190128MR_PET_RP_pos_014; RAW_FILE_NAME=190122MR_PET_RP_neg_020; RAW_FILE_NAME=190130MR_PET_Hilic_pos_014 SUBJECT_SAMPLE_FACTORS - 1 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_015; RAW_FILE_NAME=190128MR_PET_RP_pos_015; RAW_FILE_NAME=190122MR_PET_RP_neg_021; RAW_FILE_NAME=190130MR_PET_Hilic_pos_015 SUBJECT_SAMPLE_FACTORS - 3 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_017; RAW_FILE_NAME=190128MR_PET_RP_pos_017; RAW_FILE_NAME=190122MR_PET_RP_neg_023; RAW_FILE_NAME=190130MR_PET_Hilic_pos_017 SUBJECT_SAMPLE_FACTORS - 4 Treatment:warm RAW_FILE_NAME=190201MR_PET_Hilic_neg_018; RAW_FILE_NAME=190128MR_PET_RP_pos_018; RAW_FILE_NAME=190122MR_PET_RP_neg_024; RAW_FILE_NAME=190130MR_PET_Hilic_pos_018 SUBJECT_SAMPLE_FACTORS - 6 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_020; RAW_FILE_NAME=190128MR_PET_RP_pos_020; RAW_FILE_NAME=190122MR_PET_RP_neg_026; RAW_FILE_NAME=190130MR_PET_Hilic_pos_020 SUBJECT_SAMPLE_FACTORS - 7 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_021; RAW_FILE_NAME=190128MR_PET_RP_pos_021; RAW_FILE_NAME=190122MR_PET_RP_neg_027; RAW_FILE_NAME=190130MR_PET_Hilic_pos_021 SUBJECT_SAMPLE_FACTORS - 8 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_022; RAW_FILE_NAME=190128MR_PET_RP_pos_022; RAW_FILE_NAME=190122MR_PET_RP_neg_028; RAW_FILE_NAME=190130MR_PET_Hilic_pos_022 SUBJECT_SAMPLE_FACTORS - 9 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_023; RAW_FILE_NAME=190128MR_PET_RP_pos_023; RAW_FILE_NAME=190122MR_PET_RP_neg_029; RAW_FILE_NAME=190130MR_PET_Hilic_pos_023 SUBJECT_SAMPLE_FACTORS - 11 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_025; RAW_FILE_NAME=190128MR_PET_RP_pos_025; RAW_FILE_NAME=190122MR_PET_RP_neg_031; RAW_FILE_NAME=190130MR_PET_Hilic_pos_025 SUBJECT_SAMPLE_FACTORS - 12 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_026; RAW_FILE_NAME=190128MR_PET_RP_pos_026; RAW_FILE_NAME=190122MR_PET_RP_neg_032; RAW_FILE_NAME=190130MR_PET_Hilic_pos_026 SUBJECT_SAMPLE_FACTORS - QC3 Treatment:QC RAW_FILE_NAME=190201MR_PET_Hilic_neg_027; RAW_FILE_NAME=190128MR_PET_RP_pos_027; RAW_FILE_NAME=190122MR_PET_RP_neg_033; RAW_FILE_NAME=190130MR_PET_Hilic_pos_027 SUBJECT_SAMPLE_FACTORS - 13 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_028; RAW_FILE_NAME=190128MR_PET_RP_pos_028; RAW_FILE_NAME=190122MR_PET_RP_neg_034; RAW_FILE_NAME=190130MR_PET_Hilic_pos_028 SUBJECT_SAMPLE_FACTORS - 14 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_029; RAW_FILE_NAME=190128MR_PET_RP_pos_029; RAW_FILE_NAME=190122MR_PET_RP_neg_035; RAW_FILE_NAME=190130MR_PET_Hilic_pos_029 SUBJECT_SAMPLE_FACTORS - 15 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_030; RAW_FILE_NAME=190128MR_PET_RP_pos_030; RAW_FILE_NAME=190122MR_PET_RP_neg_036; RAW_FILE_NAME=190130MR_PET_Hilic_pos_030 SUBJECT_SAMPLE_FACTORS - 16 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_031; RAW_FILE_NAME=190128MR_PET_RP_pos_031; RAW_FILE_NAME=190122MR_PET_RP_neg_037; RAW_FILE_NAME=190130MR_PET_Hilic_pos_031 SUBJECT_SAMPLE_FACTORS - 17 Treatment:warm RAW_FILE_NAME=190201MR_PET_Hilic_neg_032; RAW_FILE_NAME=190128MR_PET_RP_pos_032; RAW_FILE_NAME=190122MR_PET_RP_neg_038; RAW_FILE_NAME=190130MR_PET_Hilic_pos_032 SUBJECT_SAMPLE_FACTORS - 18 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_033; RAW_FILE_NAME=190128MR_PET_RP_pos_033; RAW_FILE_NAME=190122MR_PET_RP_neg_039; RAW_FILE_NAME=190130MR_PET_Hilic_pos_033 SUBJECT_SAMPLE_FACTORS - 19 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_034; RAW_FILE_NAME=190128MR_PET_RP_pos_034; RAW_FILE_NAME=190122MR_PET_RP_neg_040; RAW_FILE_NAME=190130MR_PET_Hilic_pos_034 SUBJECT_SAMPLE_FACTORS - 20 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_035; RAW_FILE_NAME=190128MR_PET_RP_pos_035; RAW_FILE_NAME=190122MR_PET_RP_neg_041; RAW_FILE_NAME=190130MR_PET_Hilic_pos_035 SUBJECT_SAMPLE_FACTORS - 21 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_036; RAW_FILE_NAME=190128MR_PET_RP_pos_036; RAW_FILE_NAME=190122MR_PET_RP_neg_042; RAW_FILE_NAME=190130MR_PET_Hilic_pos_036 SUBJECT_SAMPLE_FACTORS - 22 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_037; RAW_FILE_NAME=190128MR_PET_RP_pos_037; RAW_FILE_NAME=190122MR_PET_RP_neg_043; RAW_FILE_NAME=190130MR_PET_Hilic_pos_037 SUBJECT_SAMPLE_FACTORS - 23 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_038; RAW_FILE_NAME=190128MR_PET_RP_pos_038; RAW_FILE_NAME=190122MR_PET_RP_neg_044; RAW_FILE_NAME=190130MR_PET_Hilic_pos_038 SUBJECT_SAMPLE_FACTORS - 24 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_039; RAW_FILE_NAME=190128MR_PET_RP_pos_039; RAW_FILE_NAME=190122MR_PET_RP_neg_045; RAW_FILE_NAME=190130MR_PET_Hilic_pos_039 SUBJECT_SAMPLE_FACTORS - QC4 Treatment:QC RAW_FILE_NAME=190201MR_PET_Hilic_neg_040; RAW_FILE_NAME=190128MR_PET_RP_pos_040; RAW_FILE_NAME=190122MR_PET_RP_neg_046; RAW_FILE_NAME=190130MR_PET_Hilic_pos_040 SUBJECT_SAMPLE_FACTORS - 25 Treatment:warm RAW_FILE_NAME=190201MR_PET_Hilic_neg_041; RAW_FILE_NAME=190128MR_PET_RP_pos_041; RAW_FILE_NAME=190122MR_PET_RP_neg_047; RAW_FILE_NAME=190130MR_PET_Hilic_pos_041 SUBJECT_SAMPLE_FACTORS - 26 Treatment:warm RAW_FILE_NAME=190201MR_PET_Hilic_neg_042; RAW_FILE_NAME=190128MR_PET_RP_pos_042; RAW_FILE_NAME=190122MR_PET_RP_neg_048; RAW_FILE_NAME=190130MR_PET_Hilic_pos_042 SUBJECT_SAMPLE_FACTORS - 27 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_043; RAW_FILE_NAME=190128MR_PET_RP_pos_043; RAW_FILE_NAME=190122MR_PET_RP_neg_049; RAW_FILE_NAME=190130MR_PET_Hilic_pos_043 SUBJECT_SAMPLE_FACTORS - 28 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_044; RAW_FILE_NAME=190128MR_PET_RP_pos_044; RAW_FILE_NAME=190122MR_PET_RP_neg_050; RAW_FILE_NAME=190130MR_PET_Hilic_pos_044 SUBJECT_SAMPLE_FACTORS - 29 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_045; RAW_FILE_NAME=190128MR_PET_RP_pos_045; RAW_FILE_NAME=190122MR_PET_RP_neg_051; RAW_FILE_NAME=190130MR_PET_Hilic_pos_045 SUBJECT_SAMPLE_FACTORS - 30 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_046; RAW_FILE_NAME=190128MR_PET_RP_pos_046; RAW_FILE_NAME=190122MR_PET_RP_neg_052; RAW_FILE_NAME=190130MR_PET_Hilic_pos_046 SUBJECT_SAMPLE_FACTORS - 31 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_047; RAW_FILE_NAME=190128MR_PET_RP_pos_047; RAW_FILE_NAME=190122MR_PET_RP_neg_053; RAW_FILE_NAME=190130MR_PET_Hilic_pos_047 SUBJECT_SAMPLE_FACTORS - 32 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_048; RAW_FILE_NAME=190128MR_PET_RP_pos_048; RAW_FILE_NAME=190122MR_PET_RP_neg_054; RAW_FILE_NAME=190130MR_PET_Hilic_pos_048 SUBJECT_SAMPLE_FACTORS - 33 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_049; RAW_FILE_NAME=190128MR_PET_RP_pos_049; RAW_FILE_NAME=190122MR_PET_RP_neg_055; RAW_FILE_NAME=190130MR_PET_Hilic_pos_049 SUBJECT_SAMPLE_FACTORS - 34 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_050; RAW_FILE_NAME=190128MR_PET_RP_pos_050; RAW_FILE_NAME=190122MR_PET_RP_neg_056; RAW_FILE_NAME=190130MR_PET_Hilic_pos_050 SUBJECT_SAMPLE_FACTORS - 35 Treatment:warm RAW_FILE_NAME=190201MR_PET_Hilic_neg_051; RAW_FILE_NAME=190128MR_PET_RP_pos_051; RAW_FILE_NAME=190122MR_PET_RP_neg_057; RAW_FILE_NAME=190130MR_PET_Hilic_pos_051 SUBJECT_SAMPLE_FACTORS - 36 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_052; RAW_FILE_NAME=190128MR_PET_RP_pos_052; RAW_FILE_NAME=190122MR_PET_RP_neg_058; RAW_FILE_NAME=190130MR_PET_Hilic_pos_052 SUBJECT_SAMPLE_FACTORS - QC5 Treatment:QC RAW_FILE_NAME=190201MR_PET_Hilic_neg_053; RAW_FILE_NAME=190128MR_PET_RP_pos_053; RAW_FILE_NAME=190122MR_PET_RP_neg_059; RAW_FILE_NAME=190130MR_PET_Hilic_pos_053 SUBJECT_SAMPLE_FACTORS - 37 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_054; RAW_FILE_NAME=190128MR_PET_RP_pos_054; RAW_FILE_NAME=190122MR_PET_RP_neg_060; RAW_FILE_NAME=190130MR_PET_Hilic_pos_054 SUBJECT_SAMPLE_FACTORS - 38 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_055; RAW_FILE_NAME=190128MR_PET_RP_pos_055; RAW_FILE_NAME=190122MR_PET_RP_neg_061; RAW_FILE_NAME=190130MR_PET_Hilic_pos_055 SUBJECT_SAMPLE_FACTORS - 39 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_056; RAW_FILE_NAME=190128MR_PET_RP_pos_056; RAW_FILE_NAME=190122MR_PET_RP_neg_062; RAW_FILE_NAME=190130MR_PET_Hilic_pos_056 SUBJECT_SAMPLE_FACTORS - 40 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_057; RAW_FILE_NAME=190128MR_PET_RP_pos_057; RAW_FILE_NAME=190122MR_PET_RP_neg_063; RAW_FILE_NAME=190130MR_PET_Hilic_pos_057 SUBJECT_SAMPLE_FACTORS - 41 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_058; RAW_FILE_NAME=190128MR_PET_RP_pos_058; RAW_FILE_NAME=190122MR_PET_RP_neg_064; RAW_FILE_NAME=190130MR_PET_Hilic_pos_058 SUBJECT_SAMPLE_FACTORS - 42 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_059; RAW_FILE_NAME=190128MR_PET_RP_pos_059; RAW_FILE_NAME=190122MR_PET_RP_neg_065; RAW_FILE_NAME=190130MR_PET_Hilic_pos_059 SUBJECT_SAMPLE_FACTORS - 43 Treatment:warm RAW_FILE_NAME=190201MR_PET_Hilic_neg_060; RAW_FILE_NAME=190128MR_PET_RP_pos_060; RAW_FILE_NAME=190122MR_PET_RP_neg_066; RAW_FILE_NAME=190130MR_PET_Hilic_pos_060 SUBJECT_SAMPLE_FACTORS - 45 Treatment:warm RAW_FILE_NAME=190201MR_PET_Hilic_neg_062; RAW_FILE_NAME=190128MR_PET_RP_pos_062; RAW_FILE_NAME=190122MR_PET_RP_neg_068; RAW_FILE_NAME=190130MR_PET_Hilic_pos_062 SUBJECT_SAMPLE_FACTORS - 46 Treatment:warm RAW_FILE_NAME=190201MR_PET_Hilic_neg_063; RAW_FILE_NAME=190128MR_PET_RP_pos_063; RAW_FILE_NAME=190122MR_PET_RP_neg_069; RAW_FILE_NAME=190130MR_PET_Hilic_pos_063 SUBJECT_SAMPLE_FACTORS - QC6 Treatment:QC RAW_FILE_NAME=190201MR_PET_Hilic_neg_066; RAW_FILE_NAME=190128MR_PET_RP_pos_066; RAW_FILE_NAME=190122MR_PET_RP_neg_072; RAW_FILE_NAME=190130MR_PET_Hilic_pos_066 SUBJECT_SAMPLE_FACTORS - 49 Treatment:warm RAW_FILE_NAME=190201MR_PET_Hilic_neg_067; RAW_FILE_NAME=190128MR_PET_RP_pos_067; RAW_FILE_NAME=190122MR_PET_RP_neg_073; RAW_FILE_NAME=190130MR_PET_Hilic_pos_067 SUBJECT_SAMPLE_FACTORS - 50 Treatment:warm RAW_FILE_NAME=190201MR_PET_Hilic_neg_068; RAW_FILE_NAME=190128MR_PET_RP_pos_068; RAW_FILE_NAME=190122MR_PET_RP_neg_074; RAW_FILE_NAME=190130MR_PET_Hilic_pos_068 SUBJECT_SAMPLE_FACTORS - 51 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_069; RAW_FILE_NAME=190128MR_PET_RP_pos_069; RAW_FILE_NAME=190122MR_PET_RP_neg_075; RAW_FILE_NAME=190130MR_PET_Hilic_pos_069 SUBJECT_SAMPLE_FACTORS - 52 Treatment:warm RAW_FILE_NAME=190201MR_PET_Hilic_neg_070; RAW_FILE_NAME=190128MR_PET_RP_pos_070; RAW_FILE_NAME=190122MR_PET_RP_neg_076; RAW_FILE_NAME=190130MR_PET_Hilic_pos_070 SUBJECT_SAMPLE_FACTORS - 53 Treatment:warm RAW_FILE_NAME=190201MR_PET_Hilic_neg_071; RAW_FILE_NAME=190128MR_PET_RP_pos_071; RAW_FILE_NAME=190122MR_PET_RP_neg_077; RAW_FILE_NAME=190130MR_PET_Hilic_pos_071 SUBJECT_SAMPLE_FACTORS - 54 Treatment:warm RAW_FILE_NAME=190201MR_PET_Hilic_neg_072; RAW_FILE_NAME=190128MR_PET_RP_pos_072; RAW_FILE_NAME=190122MR_PET_RP_neg_078; RAW_FILE_NAME=190130MR_PET_Hilic_pos_072 SUBJECT_SAMPLE_FACTORS - 55 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_073; RAW_FILE_NAME=190128MR_PET_RP_pos_073; RAW_FILE_NAME=190122MR_PET_RP_neg_079; RAW_FILE_NAME=190130MR_PET_Hilic_pos_073 SUBJECT_SAMPLE_FACTORS - 57 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_075; RAW_FILE_NAME=190128MR_PET_RP_pos_075; RAW_FILE_NAME=190122MR_PET_RP_neg_081; RAW_FILE_NAME=190130MR_PET_Hilic_pos_075 SUBJECT_SAMPLE_FACTORS - 58 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_076; RAW_FILE_NAME=190128MR_PET_RP_pos_076; RAW_FILE_NAME=190122MR_PET_RP_neg_082; RAW_FILE_NAME=190130MR_PET_Hilic_pos_076 SUBJECT_SAMPLE_FACTORS - 59 Treatment:warm RAW_FILE_NAME=190201MR_PET_Hilic_neg_077; RAW_FILE_NAME=190128MR_PET_RP_pos_077; RAW_FILE_NAME=190122MR_PET_RP_neg_083; RAW_FILE_NAME=190130MR_PET_Hilic_pos_077 SUBJECT_SAMPLE_FACTORS - 60 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_078; RAW_FILE_NAME=190128MR_PET_RP_pos_078; RAW_FILE_NAME=190122MR_PET_RP_neg_084; RAW_FILE_NAME=190130MR_PET_Hilic_pos_078 SUBJECT_SAMPLE_FACTORS - 61 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_079; RAW_FILE_NAME=190128MR_PET_RP_pos_079; RAW_FILE_NAME=190122MR_PET_RP_neg_085; RAW_FILE_NAME=190130MR_PET_Hilic_pos_079 SUBJECT_SAMPLE_FACTORS - 62 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_080; RAW_FILE_NAME=190128MR_PET_RP_pos_080; RAW_FILE_NAME=190122MR_PET_RP_neg_086; RAW_FILE_NAME=190130MR_PET_Hilic_pos_080 SUBJECT_SAMPLE_FACTORS - QC7 Treatment:QC RAW_FILE_NAME=190201MR_PET_Hilic_neg_081; RAW_FILE_NAME=190128MR_PET_RP_pos_081; RAW_FILE_NAME=190122MR_PET_RP_neg_087; RAW_FILE_NAME=190130MR_PET_Hilic_pos_081 SUBJECT_SAMPLE_FACTORS - 63 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_082; RAW_FILE_NAME=190128MR_PET_RP_pos_082; RAW_FILE_NAME=190122MR_PET_RP_neg_088; RAW_FILE_NAME=190130MR_PET_Hilic_pos_082 SUBJECT_SAMPLE_FACTORS - 64 Treatment:warm RAW_FILE_NAME=190201MR_PET_Hilic_neg_083; RAW_FILE_NAME=190128MR_PET_RP_pos_083; RAW_FILE_NAME=190122MR_PET_RP_neg_089; RAW_FILE_NAME=190130MR_PET_Hilic_pos_083 SUBJECT_SAMPLE_FACTORS - 65 Treatment:warm RAW_FILE_NAME=190201MR_PET_Hilic_neg_084; RAW_FILE_NAME=190128MR_PET_RP_pos_084; RAW_FILE_NAME=190122MR_PET_RP_neg_090; RAW_FILE_NAME=190130MR_PET_Hilic_pos_084 SUBJECT_SAMPLE_FACTORS - 66 Treatment:warm RAW_FILE_NAME=190201MR_PET_Hilic_neg_085; RAW_FILE_NAME=190128MR_PET_RP_pos_085; RAW_FILE_NAME=190122MR_PET_RP_neg_091; RAW_FILE_NAME=190130MR_PET_Hilic_pos_085 SUBJECT_SAMPLE_FACTORS - 67 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_086; RAW_FILE_NAME=190128MR_PET_RP_pos_086; RAW_FILE_NAME=190122MR_PET_RP_neg_092; RAW_FILE_NAME=190130MR_PET_Hilic_pos_086 SUBJECT_SAMPLE_FACTORS - 68 Treatment:warm RAW_FILE_NAME=190201MR_PET_Hilic_neg_087; RAW_FILE_NAME=190128MR_PET_RP_pos_087; RAW_FILE_NAME=190122MR_PET_RP_neg_093; RAW_FILE_NAME=190130MR_PET_Hilic_pos_087 SUBJECT_SAMPLE_FACTORS - 69 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_088; RAW_FILE_NAME=190128MR_PET_RP_pos_088; RAW_FILE_NAME=190122MR_PET_RP_neg_094; RAW_FILE_NAME=190130MR_PET_Hilic_pos_088 SUBJECT_SAMPLE_FACTORS - 70 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_089; RAW_FILE_NAME=190128MR_PET_RP_pos_089; RAW_FILE_NAME=190122MR_PET_RP_neg_095; RAW_FILE_NAME=190130MR_PET_Hilic_pos_089 SUBJECT_SAMPLE_FACTORS - 71 Treatment:warm RAW_FILE_NAME=190201MR_PET_Hilic_neg_090; RAW_FILE_NAME=190128MR_PET_RP_pos_090; RAW_FILE_NAME=190122MR_PET_RP_neg_096; RAW_FILE_NAME=190130MR_PET_Hilic_pos_090 SUBJECT_SAMPLE_FACTORS - 72 Treatment:warm RAW_FILE_NAME=190201MR_PET_Hilic_neg_091; RAW_FILE_NAME=190128MR_PET_RP_pos_091; RAW_FILE_NAME=190122MR_PET_RP_neg_097; RAW_FILE_NAME=190130MR_PET_Hilic_pos_091 SUBJECT_SAMPLE_FACTORS - 73 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_092; RAW_FILE_NAME=190128MR_PET_RP_pos_092; RAW_FILE_NAME=190122MR_PET_RP_neg_098; RAW_FILE_NAME=190130MR_PET_Hilic_pos_092 SUBJECT_SAMPLE_FACTORS - QC8 Treatment:QC RAW_FILE_NAME=190201MR_PET_Hilic_neg_094; RAW_FILE_NAME=190128MR_PET_RP_pos_094; RAW_FILE_NAME=190122MR_PET_RP_neg_100; RAW_FILE_NAME=190130MR_PET_Hilic_pos_094 SUBJECT_SAMPLE_FACTORS - 75 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_095; RAW_FILE_NAME=190128MR_PET_RP_pos_095; RAW_FILE_NAME=190122MR_PET_RP_neg_101; RAW_FILE_NAME=190130MR_PET_Hilic_pos_095 SUBJECT_SAMPLE_FACTORS - 76 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_096; RAW_FILE_NAME=190128MR_PET_RP_pos_096; RAW_FILE_NAME=190122MR_PET_RP_neg_102; RAW_FILE_NAME=190130MR_PET_Hilic_pos_096 SUBJECT_SAMPLE_FACTORS - 77 Treatment:warm RAW_FILE_NAME=190201MR_PET_Hilic_neg_097; RAW_FILE_NAME=190128MR_PET_RP_pos_097; RAW_FILE_NAME=190122MR_PET_RP_neg_103; RAW_FILE_NAME=190130MR_PET_Hilic_pos_097 SUBJECT_SAMPLE_FACTORS - 78 Treatment:warm RAW_FILE_NAME=190201MR_PET_Hilic_neg_098; RAW_FILE_NAME=190128MR_PET_RP_pos_098; RAW_FILE_NAME=190122MR_PET_RP_neg_104; RAW_FILE_NAME=190130MR_PET_Hilic_pos_098 SUBJECT_SAMPLE_FACTORS - 80 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_100; RAW_FILE_NAME=190128MR_PET_RP_pos_100; RAW_FILE_NAME=190122MR_PET_RP_neg_106; RAW_FILE_NAME=190130MR_PET_Hilic_pos_100 SUBJECT_SAMPLE_FACTORS - 81 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_101; RAW_FILE_NAME=190128MR_PET_RP_pos_101; RAW_FILE_NAME=190122MR_PET_RP_neg_107; RAW_FILE_NAME=190130MR_PET_Hilic_pos_101 SUBJECT_SAMPLE_FACTORS - 83 Treatment:warm RAW_FILE_NAME=190201MR_PET_Hilic_neg_103; RAW_FILE_NAME=190128MR_PET_RP_pos_103; RAW_FILE_NAME=190122MR_PET_RP_neg_109; RAW_FILE_NAME=190130MR_PET_Hilic_pos_103 SUBJECT_SAMPLE_FACTORS - 84 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_104; RAW_FILE_NAME=190128MR_PET_RP_pos_104; RAW_FILE_NAME=190122MR_PET_RP_neg_110; RAW_FILE_NAME=190130MR_PET_Hilic_pos_104 SUBJECT_SAMPLE_FACTORS - 85 Treatment:warm RAW_FILE_NAME=190201MR_PET_Hilic_neg_105; RAW_FILE_NAME=190128MR_PET_RP_pos_105; RAW_FILE_NAME=190122MR_PET_RP_neg_111; RAW_FILE_NAME=190130MR_PET_Hilic_pos_105 SUBJECT_SAMPLE_FACTORS - 86 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_106; RAW_FILE_NAME=190128MR_PET_RP_pos_106; RAW_FILE_NAME=190122MR_PET_RP_neg_112; RAW_FILE_NAME=190130MR_PET_Hilic_pos_106 SUBJECT_SAMPLE_FACTORS - QC9 Treatment:QC RAW_FILE_NAME=190201MR_PET_Hilic_neg_107; RAW_FILE_NAME=190128MR_PET_RP_pos_107; RAW_FILE_NAME=190122MR_PET_RP_neg_113; RAW_FILE_NAME=190130MR_PET_Hilic_pos_107 SUBJECT_SAMPLE_FACTORS - 87 Treatment:warm RAW_FILE_NAME=190201MR_PET_Hilic_neg_108; RAW_FILE_NAME=190128MR_PET_RP_pos_108; RAW_FILE_NAME=190122MR_PET_RP_neg_114; RAW_FILE_NAME=190130MR_PET_Hilic_pos_108 SUBJECT_SAMPLE_FACTORS - 88 Treatment:warm RAW_FILE_NAME=190201MR_PET_Hilic_neg_109; RAW_FILE_NAME=190128MR_PET_RP_pos_109; RAW_FILE_NAME=190122MR_PET_RP_neg_115; RAW_FILE_NAME=190130MR_PET_Hilic_pos_109 SUBJECT_SAMPLE_FACTORS - 89 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_110; RAW_FILE_NAME=190128MR_PET_RP_pos_110; RAW_FILE_NAME=190122MR_PET_RP_neg_116; RAW_FILE_NAME=190130MR_PET_Hilic_pos_110 SUBJECT_SAMPLE_FACTORS - 91 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_112; RAW_FILE_NAME=190128MR_PET_RP_pos_112; RAW_FILE_NAME=190122MR_PET_RP_neg_118; RAW_FILE_NAME=190130MR_PET_Hilic_pos_112 SUBJECT_SAMPLE_FACTORS - 92 Treatment:warm RAW_FILE_NAME=190201MR_PET_Hilic_neg_113; RAW_FILE_NAME=190128MR_PET_RP_pos_113; RAW_FILE_NAME=190122MR_PET_RP_neg_119; RAW_FILE_NAME=190130MR_PET_Hilic_pos_113 SUBJECT_SAMPLE_FACTORS - 93 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_114; RAW_FILE_NAME=190128MR_PET_RP_pos_114; RAW_FILE_NAME=190122MR_PET_RP_neg_120; RAW_FILE_NAME=190130MR_PET_Hilic_pos_114 SUBJECT_SAMPLE_FACTORS - 94 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_115; RAW_FILE_NAME=190128MR_PET_RP_pos_115; RAW_FILE_NAME=190122MR_PET_RP_neg_121; RAW_FILE_NAME=190130MR_PET_Hilic_pos_115 SUBJECT_SAMPLE_FACTORS - 95 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_116; RAW_FILE_NAME=190128MR_PET_RP_pos_116; RAW_FILE_NAME=190122MR_PET_RP_neg_122; RAW_FILE_NAME=190130MR_PET_Hilic_pos_116 SUBJECT_SAMPLE_FACTORS - 96 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_117; RAW_FILE_NAME=190128MR_PET_RP_pos_117; RAW_FILE_NAME=190122MR_PET_RP_neg_123; RAW_FILE_NAME=190130MR_PET_Hilic_pos_117 SUBJECT_SAMPLE_FACTORS - 97 Treatment:warm RAW_FILE_NAME=190201MR_PET_Hilic_neg_118; RAW_FILE_NAME=190128MR_PET_RP_pos_118; RAW_FILE_NAME=190122MR_PET_RP_neg_124; RAW_FILE_NAME=190130MR_PET_Hilic_pos_118 SUBJECT_SAMPLE_FACTORS - 98 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_119; RAW_FILE_NAME=190128MR_PET_RP_pos_119; RAW_FILE_NAME=190122MR_PET_RP_neg_125; RAW_FILE_NAME=190130MR_PET_Hilic_pos_119 SUBJECT_SAMPLE_FACTORS - 99 Treatment:cold RAW_FILE_NAME=190201MR_PET_Hilic_neg_120; RAW_FILE_NAME=190128MR_PET_RP_pos_120; RAW_FILE_NAME=190122MR_PET_RP_neg_126; RAW_FILE_NAME=190130MR_PET_Hilic_pos_120 SUBJECT_SAMPLE_FACTORS - 100 Treatment:warm RAW_FILE_NAME=190201MR_PET_Hilic_neg_121; RAW_FILE_NAME=190128MR_PET_RP_pos_121; RAW_FILE_NAME=190122MR_PET_RP_neg_127; RAW_FILE_NAME=190130MR_PET_Hilic_pos_121 SUBJECT_SAMPLE_FACTORS - 101 Treatment:warm RAW_FILE_NAME=190201MR_PET_Hilic_neg_122; RAW_FILE_NAME=190128MR_PET_RP_pos_122; RAW_FILE_NAME=190122MR_PET_RP_neg_128; RAW_FILE_NAME=190130MR_PET_Hilic_pos_122 SUBJECT_SAMPLE_FACTORS - 102 Treatment:warm RAW_FILE_NAME=190201MR_PET_Hilic_neg_123; RAW_FILE_NAME=190128MR_PET_RP_pos_123; RAW_FILE_NAME=190122MR_PET_RP_neg_129; RAW_FILE_NAME=190130MR_PET_Hilic_pos_123 SUBJECT_SAMPLE_FACTORS - QC10 Treatment:QC RAW_FILE_NAME=190201MR_PET_Hilic_neg_124; RAW_FILE_NAME=190128MR_PET_RP_pos_124; RAW_FILE_NAME=190122MR_PET_RP_neg_130; RAW_FILE_NAME=190130MR_PET_Hilic_pos_124 SUBJECT_SAMPLE_FACTORS - QC autoMSMS 10V_2 Treatment:QC RAW_FILE_NAME=190201MR_PET_Hilic_neg_125; RAW_FILE_NAME=190128MR_PET_RP_pos_125; RAW_FILE_NAME=190122MR_PET_RP_neg_131; RAW_FILE_NAME=190130MR_PET_Hilic_pos_125 SUBJECT_SAMPLE_FACTORS - QC autoMSMS 20V_2 Treatment:QC RAW_FILE_NAME=190201MR_PET_Hilic_neg_126; RAW_FILE_NAME=190128MR_PET_RP_pos_126; RAW_FILE_NAME=190122MR_PET_RP_neg_132; RAW_FILE_NAME=190130MR_PET_Hilic_pos_126 SUBJECT_SAMPLE_FACTORS - QC autoMSMS 40V_2 Treatment:QC RAW_FILE_NAME=190201MR_PET_Hilic_neg_127; RAW_FILE_NAME=190128MR_PET_RP_pos_127; RAW_FILE_NAME=190122MR_PET_RP_neg_133; RAW_FILE_NAME=190130MR_PET_Hilic_pos_127 #COLLECTION CO:COLLECTION_SUMMARY Blood samples were either taken during the exposure to room temperature or CO:COLLECTION_SUMMARY during cold (subsequent to 2 hours of personalised cooling). More details are in CO:COLLECTION_SUMMARY the manuscript. CO:SAMPLE_TYPE Blood (serum) CO:STORAGE_CONDITIONS -80℃ #TREATMENT TR:TREATMENT_SUMMARY The exposure to cold for 2 hours under cooling blankets using a personalised TR:TREATMENT_SUMMARY cooling protocol #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Fasting serum samples taken during the cold or RT exposures were stored at -80 SP:SAMPLEPREP_SUMMARY °C and thawed in an ice water bath. Four hundred microliters of ice-cold SP:SAMPLEPREP_SUMMARY acetonitrile (ACN) was pipetted to 96-well filter plates (Captiva ND Plate, 0.2 SP:SAMPLEPREP_SUMMARY μm PP, Agilent Technologies, Santa Clara, CA, United States) after which 100 SP:SAMPLEPREP_SUMMARY μL of plasma or quality control (QC) was added. The solution was mixed by SP:SAMPLEPREP_SUMMARY pipetting up and down, filtrated by centrifugation (700 RCF, 4 °C, 5 min) and SP:SAMPLEPREP_SUMMARY collected to a 96-1 well plate (96 deep well plate natural,Thermo Scientific). SP:SAMPLEPREP_SUMMARY QC was serum sample prepared by pooling an aliquot from all analysed serum SP:SAMPLEPREP_SUMMARY samples. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Agilent 1290 CH:COLUMN_NAME Agilent Zorbax Eclipse XDB-C18 Rapid Resolution HD 1.8 μm, 2.1 x 100 mm CH:SOLVENT_A water with 0.1% (v/v) of formic acid CH:SOLVENT_B methanol with 0.1% (v/v) of formic acid CH:FLOW_GRADIENT 2 to 100% B in 10 min; 100% B from 10 min to 14.5 min; 100% to 2% B, from 14.51 CH:FLOW_GRADIENT min to 16.5 min CH:FLOW_RATE 0.4 mL/min. CH:COLUMN_TEMPERATURE 50 #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Agilent 6540 QTOF MS:INSTRUMENT_TYPE QTOF MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:MS_COMMENTS The data acquisition software was the MassHunter Acquisition B.05.01 (Agilent MS:MS_COMMENTS Technologies). MS:MS_RESULTS_FILE ST002826_AN004612_Results.txt UNITS:Area Has m/z:Yes Has RT:Yes RT units:Minutes #END