#METABOLOMICS WORKBENCH mlafrano_20150924_9047701_mwtab.txt DATATRACK_ID:384 STUDY_ID:ST000257 ANALYSIS_ID:AN000408 PROJECT_ID:PR000209
VERSION             	1
CREATED_ON          	2016-09-17
#PROJECT
PR:PROJECT_TITLE                 	Metabolite changes associated with weight loss
PR:PROJECT_TYPE                  	Feeding study
PR:PROJECT_SUMMARY               	This study aims to identify changes in metabolites that occur in metabolic
PR:PROJECT_SUMMARY               	including liver, adipose (epididymal), hypothalamus, and muscle, as well as
PR:PROJECT_SUMMARY               	in the obese state that are long-lasting and not reversed by weight loss. Mice
PR:PROJECT_SUMMARY               	divided into three groups termed LFD (low fat diet), HFD (high fat diet), and
PR:PROJECT_SUMMARY               	(switch diet from HFD to LFD)(n = 6 per group). All mice were fed normal chow
PR:PROJECT_SUMMARY               	12 weeks of age. The LFD group were fed a low fat diet (10% fat) ad libitum for
PR:PROJECT_SUMMARY               	weeks. The HFD diet group were fed high fat diet (60% fat) ad libitum for 18
PR:PROJECT_SUMMARY               	The SW group were fed high fat diet for 9 weeks, then low fat diet for 9 weeks
PR:PROJECT_SUMMARY               	which their body weights returned to similar levels as the LFD group. Each
PR:PROJECT_SUMMARY               	was analyzed for metabolite and gene expression changes.
PR:INSTITUTE                     	University of California, San Diego
PR:DEPARTMENT                    	Department of Medicine
PR:LABORATORY                    	Osborn
PR:LAST_NAME                     	Osborn
PR:FIRST_NAME                    	Olivia
PR:ADDRESS                       	9500 Gilman Dr., La Jolla, CA 92093
PR:EMAIL                         	oosborn@ucsd.edu
PR:PHONE                         	+1-858-822-6645
PR:FUNDING_SOURCE                	National Institute of Health U24 DK092993
#STUDY
ST:STUDY_TITLE                   	NIH WCMC Pilot & Feasibility Project: “Metabolite changes associated with
ST:STUDY_TITLE                   	loss”
ST:STUDY_TYPE                    	Feeding study
ST:STUDY_SUMMARY                 	Targeted metabolomic analyses of oxylipins, endocannabinoids, and ceramides
ST:STUDY_SUMMARY                 	performed on 18 mouse liver, adipose, hypothalamus, plasma, and muscle samples
ST:STUDY_SUMMARY                 	from mice euthanized at 18 weeks following consumption of three diet regimens
ST:STUDY_SUMMARY                 	induced lean, obese, and weight loss phenotypes. Samples were analyzed by
ST:STUDY_SUMMARY                 	using a Waters Acquity UPLC and detected on an API 4000 QTrap (AB Sciex,
ST:STUDY_SUMMARY                 	MA, USA) by multiple reaction monitoring (MRM) after negative mode electrospray
ST:INSTITUTE                     	University of California, Davis
ST:DEPARTMENT                    	Nutrition
ST:LABORATORY                    	Newman Lab
ST:LAST_NAME                     	Newman
ST:FIRST_NAME                    	John
ST:ADDRESS                       	430 W. Health Sciences Dr., Davis, CA 95616
ST:EMAIL                         	john.newman@ars.usda.gov
ST:PHONE                         	+1-530-752-1009
ST:NUM_GROUPS                    	3
ST:TOTAL_SUBJECTS                	18
#SUBJECT
SU:SUBJECT_TYPE                  	Animal
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
SU:AGE_OR_AGE_RANGE              	30 weeks
SU:GENDER                        	Female
SU:ANIMAL_HOUSING                	Singly housed
SU:ANIMAL_FEED                   	High fat diet (D12492, Research Diets, New Brunswick, NJ), Low fat diet
SU:ANIMAL_FEED                   	Research Diets, New Brunswick, NJ)
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	-	HF epi 1	Treatment:HFD | Tissue Type:Adipose	
SUBJECT_SAMPLE_FACTORS           	-	HF epi 2	Treatment:HFD | Tissue Type:Adipose	
SUBJECT_SAMPLE_FACTORS           	-	HF epi 3	Treatment:HFD | Tissue Type:Adipose	
SUBJECT_SAMPLE_FACTORS           	-	HF epi 4	Treatment:HFD | Tissue Type:Adipose	
SUBJECT_SAMPLE_FACTORS           	-	HF epi 7	Treatment:HFD | Tissue Type:Adipose	
SUBJECT_SAMPLE_FACTORS           	-	HF epi 8	Treatment:HFD | Tissue Type:Adipose	
SUBJECT_SAMPLE_FACTORS           	-	HF Hypo 1	Treatment:HFD | Tissue Type:Hypothalmus	
SUBJECT_SAMPLE_FACTORS           	-	HF Hypo 2	Treatment:HFD | Tissue Type:Hypothalmus	
SUBJECT_SAMPLE_FACTORS           	-	HF Hypo 3	Treatment:HFD | Tissue Type:Hypothalmus	
SUBJECT_SAMPLE_FACTORS           	-	HF Hypo 4	Treatment:HFD | Tissue Type:Hypothalmus	
SUBJECT_SAMPLE_FACTORS           	-	HF Hypo 7	Treatment:HFD | Tissue Type:Hypothalmus	
SUBJECT_SAMPLE_FACTORS           	-	HF Hypo 8	Treatment:HFD | Tissue Type:Hypothalmus	
SUBJECT_SAMPLE_FACTORS           	-	HF liv 1	Treatment:HFD | Tissue Type:Liver	
SUBJECT_SAMPLE_FACTORS           	-	HF liv 2	Treatment:HFD | Tissue Type:Liver	
SUBJECT_SAMPLE_FACTORS           	-	HF liv 3	Treatment:HFD | Tissue Type:Liver	
SUBJECT_SAMPLE_FACTORS           	-	HF liv 4	Treatment:HFD | Tissue Type:Liver	
SUBJECT_SAMPLE_FACTORS           	-	HF liv 7	Treatment:HFD | Tissue Type:Liver	
SUBJECT_SAMPLE_FACTORS           	-	HF liv 8	Treatment:HFD | Tissue Type:Liver	
SUBJECT_SAMPLE_FACTORS           	-	HF mus 1	Treatment:HFD | Tissue Type:Muscle	
SUBJECT_SAMPLE_FACTORS           	-	HF mus 2	Treatment:HFD | Tissue Type:Muscle	
SUBJECT_SAMPLE_FACTORS           	-	HF mus 3	Treatment:HFD | Tissue Type:Muscle	
SUBJECT_SAMPLE_FACTORS           	-	HF mus 4	Treatment:HFD | Tissue Type:Muscle	
SUBJECT_SAMPLE_FACTORS           	-	HF mus 7	Treatment:HFD | Tissue Type:Muscle	
SUBJECT_SAMPLE_FACTORS           	-	HF mus 8	Treatment:HFD | Tissue Type:Muscle	
SUBJECT_SAMPLE_FACTORS           	-	HF plasma 1	Treatment:HFD | Tissue Type:Plasma	
SUBJECT_SAMPLE_FACTORS           	-	HF plasma 2	Treatment:HFD | Tissue Type:Plasma	
SUBJECT_SAMPLE_FACTORS           	-	HF plasma 3	Treatment:HFD | Tissue Type:Plasma	
SUBJECT_SAMPLE_FACTORS           	-	HF plasma 4	Treatment:HFD | Tissue Type:Plasma	
SUBJECT_SAMPLE_FACTORS           	-	HF plasma 7	Treatment:HFD | Tissue Type:Plasma	
SUBJECT_SAMPLE_FACTORS           	-	HF plasma 8	Treatment:HFD | Tissue Type:Plasma	
SUBJECT_SAMPLE_FACTORS           	-	LF epi 1	Treatment:LFD | Tissue Type:Adipose	
SUBJECT_SAMPLE_FACTORS           	-	LF epi 2	Treatment:LFD | Tissue Type:Adipose	
SUBJECT_SAMPLE_FACTORS           	-	LF epi 3	Treatment:LFD | Tissue Type:Adipose	
SUBJECT_SAMPLE_FACTORS           	-	LF epi 6	Treatment:LFD | Tissue Type:Adipose	
SUBJECT_SAMPLE_FACTORS           	-	LF epi 7	Treatment:LFD | Tissue Type:Adipose	
SUBJECT_SAMPLE_FACTORS           	-	LF epi 8	Treatment:LFD | Tissue Type:Adipose	
SUBJECT_SAMPLE_FACTORS           	-	LF Hypo 1	Treatment:LFD | Tissue Type:Hypothalmus	
SUBJECT_SAMPLE_FACTORS           	-	LF Hypo 2	Treatment:LFD | Tissue Type:Hypothalmus	
SUBJECT_SAMPLE_FACTORS           	-	LF Hypo 3	Treatment:LFD | Tissue Type:Hypothalmus	
SUBJECT_SAMPLE_FACTORS           	-	LF Hypo 6	Treatment:LFD | Tissue Type:Hypothalmus	
SUBJECT_SAMPLE_FACTORS           	-	LF Hypo 7	Treatment:LFD | Tissue Type:Hypothalmus	
SUBJECT_SAMPLE_FACTORS           	-	LF Hypo 8	Treatment:LFD | Tissue Type:Hypothalmus	
SUBJECT_SAMPLE_FACTORS           	-	LF liv 1	Treatment:LFD | Tissue Type:Liver	
SUBJECT_SAMPLE_FACTORS           	-	LF liv 2	Treatment:LFD | Tissue Type:Liver	
SUBJECT_SAMPLE_FACTORS           	-	LF liv 3	Treatment:LFD | Tissue Type:Liver	
SUBJECT_SAMPLE_FACTORS           	-	LF liv 6	Treatment:LFD | Tissue Type:Liver	
SUBJECT_SAMPLE_FACTORS           	-	LF liv 7	Treatment:LFD | Tissue Type:Liver	
SUBJECT_SAMPLE_FACTORS           	-	LF liv 8	Treatment:LFD | Tissue Type:Liver	
SUBJECT_SAMPLE_FACTORS           	-	LF mus 1	Treatment:LFD | Tissue Type:Muscle	
SUBJECT_SAMPLE_FACTORS           	-	LF mus 2	Treatment:LFD | Tissue Type:Muscle	
SUBJECT_SAMPLE_FACTORS           	-	LF mus 3	Treatment:LFD | Tissue Type:Muscle	
SUBJECT_SAMPLE_FACTORS           	-	LF mus 6	Treatment:LFD | Tissue Type:Muscle	
SUBJECT_SAMPLE_FACTORS           	-	LF mus 7	Treatment:LFD | Tissue Type:Muscle	
SUBJECT_SAMPLE_FACTORS           	-	LF mus 8	Treatment:LFD | Tissue Type:Muscle	
SUBJECT_SAMPLE_FACTORS           	-	LF plasma 1	Treatment:LFD | Tissue Type:Plasma	
SUBJECT_SAMPLE_FACTORS           	-	LF plasma 2	Treatment:LFD | Tissue Type:Plasma	
SUBJECT_SAMPLE_FACTORS           	-	LF plasma 3	Treatment:LFD | Tissue Type:Plasma	
SUBJECT_SAMPLE_FACTORS           	-	LF plasma 6	Treatment:LFD | Tissue Type:Plasma	
SUBJECT_SAMPLE_FACTORS           	-	LF plasma 7	Treatment:LFD | Tissue Type:Plasma	
SUBJECT_SAMPLE_FACTORS           	-	LF plasma 8	Treatment:LFD | Tissue Type:Plasma	
SUBJECT_SAMPLE_FACTORS           	-	SW epi 2	Treatment:SW | Tissue Type:Adipose	
SUBJECT_SAMPLE_FACTORS           	-	SW epi 3	Treatment:SW | Tissue Type:Adipose	
SUBJECT_SAMPLE_FACTORS           	-	SW epi 4	Treatment:SW | Tissue Type:Adipose	
SUBJECT_SAMPLE_FACTORS           	-	SW epi 5	Treatment:SW | Tissue Type:Adipose	
SUBJECT_SAMPLE_FACTORS           	-	SW epi 7	Treatment:SW | Tissue Type:Adipose	
SUBJECT_SAMPLE_FACTORS           	-	SW epi 8	Treatment:SW | Tissue Type:Adipose	
SUBJECT_SAMPLE_FACTORS           	-	SW Hypo 2	Treatment:SW | Tissue Type:Hypothalmus	
SUBJECT_SAMPLE_FACTORS           	-	SW Hypo 3	Treatment:SW | Tissue Type:Hypothalmus	
SUBJECT_SAMPLE_FACTORS           	-	SW Hypo 4	Treatment:SW | Tissue Type:Hypothalmus	
SUBJECT_SAMPLE_FACTORS           	-	SW Hypo 5	Treatment:SW | Tissue Type:Hypothalmus	
SUBJECT_SAMPLE_FACTORS           	-	SW Hypo 7	Treatment:SW | Tissue Type:Hypothalmus	
SUBJECT_SAMPLE_FACTORS           	-	SW Hypo 8	Treatment:SW | Tissue Type:Hypothalmus	
SUBJECT_SAMPLE_FACTORS           	-	SW liv 2	Treatment:SW | Tissue Type:Liver	
SUBJECT_SAMPLE_FACTORS           	-	SW liv 3	Treatment:SW | Tissue Type:Liver	
SUBJECT_SAMPLE_FACTORS           	-	SW liv 4	Treatment:SW | Tissue Type:Liver	
SUBJECT_SAMPLE_FACTORS           	-	SW liv 5	Treatment:SW | Tissue Type:Liver	
SUBJECT_SAMPLE_FACTORS           	-	SW liv 7	Treatment:SW | Tissue Type:Liver	
SUBJECT_SAMPLE_FACTORS           	-	SW liv 8	Treatment:SW | Tissue Type:Liver	
SUBJECT_SAMPLE_FACTORS           	-	SW mus 2	Treatment:SW | Tissue Type:Muscle	
SUBJECT_SAMPLE_FACTORS           	-	SW mus 3	Treatment:SW | Tissue Type:Muscle	
SUBJECT_SAMPLE_FACTORS           	-	SW mus 4	Treatment:SW | Tissue Type:Muscle	
SUBJECT_SAMPLE_FACTORS           	-	SW mus 5	Treatment:SW | Tissue Type:Muscle	
SUBJECT_SAMPLE_FACTORS           	-	SW mus 7	Treatment:SW | Tissue Type:Muscle	
SUBJECT_SAMPLE_FACTORS           	-	SW mus 8	Treatment:SW | Tissue Type:Muscle	
SUBJECT_SAMPLE_FACTORS           	-	SW plasma 2	Treatment:SW | Tissue Type:Plasma	
SUBJECT_SAMPLE_FACTORS           	-	SW plasma 3	Treatment:SW | Tissue Type:Plasma	
SUBJECT_SAMPLE_FACTORS           	-	SW plasma 4	Treatment:SW | Tissue Type:Plasma	
SUBJECT_SAMPLE_FACTORS           	-	SW plasma 5	Treatment:SW | Tissue Type:Plasma	
SUBJECT_SAMPLE_FACTORS           	-	SW plasma 7	Treatment:SW | Tissue Type:Plasma	
SUBJECT_SAMPLE_FACTORS           	-	SW plasma 8	Treatment:SW | Tissue Type:Plasma	
#COLLECTION
CO:COLLECTION_SUMMARY            	-
CO:COLLECTION_PROTOCOL_FILENAME  	NewmanLab-Mediators-Protocol-PT.pdf
CO:SAMPLE_TYPE                   	Liver, adipose, hypothalamus, muscle, and plasma
CO:COLLECTION_METHOD             	Dissection
CO:STORAGE_CONDITIONS            	- 80°C
CO:STORAGE_VIALS                 	Eppendorf tubes
#TREATMENT
TR:TREATMENT_SUMMARY             	Mice were fed 18 weeks on either a low fat diet (LF), high fat diet (HF), or
TR:TREATMENT_SUMMARY             	of both (SW).
TR:TREATMENT_PROTOCOL_FILENAME   	NewmanLab-Mediators-Protocol-PT.pdf
TR:TREATMENT                     	Intervention
TR:TREATMENT_DOSEDURATION        	18 weeks
TR:TREATMENT_VEHICLE             	animal feed
TR:ANIMAL_ENDP_EUTHANASIA        	30 weeks
TR:ANIMAL_ENDP_TISSUE_COLL_LIST  	Liver, adipose, hypothalami, muscle, and plasma
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Tissues were pulverized on dry ice, enriched with deuterated surrogates,
SP:SAMPLEPREP_SUMMARY            	(BHT and EDTA), and extracted with MeOH:Water:IPA, transferred to an Ostro
SP:SAMPLEPREP_SUMMARY            	and the extract was eluted by vacuum. Plasma was added directly to the plate,
SP:SAMPLEPREP_SUMMARY            	with the surrogates, antioxidants, and IPA and the extract eluted through. The
SP:SAMPLEPREP_SUMMARY            	were dried, reconstituted in 100?L 50:50 methanol:acetonitrile with internal
SP:SAMPLEPREP_SUMMARY            	1?phenyl?3?hexanoic acid urea and 1?cyclohexyl?3-dodecanoic acid urea
SP:SAMPLEPREP_SUMMARY            	St. Louis, MO) and filtered at 0.1 µm.
SP:SAMPLEPREP_PROTOCOL_FILENAME  	NewmanLab-Mediators-Protocol-PT.pdf
SP:EXTRACTION_METHOD             	Ostro plate
SP:EXTRACT_CLEANUP               	Ostro plate
SP:EXTRACT_STORAGE               	- 20 °C
SP:SAMPLE_RESUSPENSION           	100 µL
SP:SAMPLE_SPIKING                	See sample prep protocol file
SP:ORGAN                         	Liver, adipose, hypothalamus, muscle, and plasma
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	Ceramide analysis
CH:CHROMATOGRAPHY_TYPE           	-
CH:INSTRUMENT_NAME               	Waters Acquity
CH:COLUMN_NAME                   	-
CH:METHODS_FILENAME              	NewmanLab-Mediators-Protocol-PT.pdf
CH:COLUMN_TEMPERATURE            	60 C
CH:FLOW_GRADIENT                 	See protocol/methods file
CH:FLOW_RATE                     	0.25
CH:INTERNAL_STANDARD             	See protocol/methods file
CH:RETENTION_TIME                	See protocol/methods file
CH:SAMPLE_INJECTION              	5 L
CH:SOLVENT_A                     	5 mm NH4COO 0.2% formic acid
CH:SOLVENT_B                     	5 mm NH4COO 0.2% formic acid in MeOH
CH:ANALYTICAL_TIME               	15 min
CH:WEAK_WASH_SOLVENT_NAME        	20% methanol, 10% isopropanol
CH:WEAK_WASH_VOLUME              	600 L
CH:STRONG_WASH_SOLVENT_NAME      	50:50 Acetonitrile:Methanol
CH:STRONG_WASH_VOLUME            	600 L
CH:SAMPLE_LOOP_SIZE              	17 L
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
AN:LABORATORY_NAME               	Newman
AN:SOFTWARE_VERSION              	AB Sciex MultiQuant version 3.0.1
AN:OPERATOR_NAME                 	Michael La Frano
AN:DETECTOR_TYPE                 	API 4000 QTrap (AB Sciex, Framingham, MA, USA)
AN:DATA_FORMAT                   	.mzML
#MS
MS:INSTRUMENT_NAME               	ABI Sciex API 4000 QTrap
MS:INSTRUMENT_TYPE               	Triple quadrupole
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:CAPILLARY_TEMPERATURE         	See protocol file
MS:CAPILLARY_VOLTAGE             	Positive
MS:MS_COMMENTS                   	Ceramide analysis
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS         	nM
MS_METABOLITE_DATA_START
Samples	HF epi 1	HF epi 2	HF epi 3	HF epi 4	HF epi 7	HF epi 8	HF Hypo 1	HF Hypo 2	HF Hypo 3	HF Hypo 4	HF Hypo 7	HF Hypo 8	HF liv 1	HF liv 2	HF liv 3	HF liv 4	HF liv 7	HF liv 8	HF mus 1	HF mus 2	HF mus 3	HF mus 4	HF mus 7	HF mus 8	HF plasma 1	HF plasma 2	HF plasma 3	HF plasma 4	HF plasma 7	HF plasma 8	LF epi 1	LF epi 2	LF epi 3	LF epi 6	LF epi 7	LF epi 8	LF Hypo 1	LF Hypo 2	LF Hypo 3	LF Hypo 6	LF Hypo 7	LF Hypo 8	LF liv 1	LF liv 2	LF liv 3	LF liv 6	LF liv 7	LF liv 8	LF mus 1	LF mus 2	LF mus 3	LF mus 6	LF mus 7	LF mus 8	LF plasma 1	LF plasma 2	LF plasma 3	LF plasma 6	LF plasma 7	LF plasma 8	SW epi 2	SW epi 3	SW epi 4	SW epi 5	SW epi 7	SW epi 8	SW Hypo 2	SW Hypo 3	SW Hypo 4	SW Hypo 5	SW Hypo 7	SW Hypo 8	SW liv 2	SW liv 3	SW liv 4	SW liv 5	SW liv 7	SW liv 8	SW mus 2	SW mus 3	SW mus 4	SW mus 5	SW mus 7	SW mus 8	SW plasma 2	SW plasma 3	SW plasma 4	SW plasma 5	SW plasma 7	SW plasma 8
Factors	Treatment:HFD | Tissue Type:Adipose	Treatment:HFD | Tissue Type:Adipose	Treatment:HFD | Tissue Type:Adipose	Treatment:HFD | Tissue Type:Adipose	Treatment:HFD | Tissue Type:Adipose	Treatment:HFD | Tissue Type:Adipose	Treatment:HFD | Tissue Type:Hypothalmus	Treatment:HFD | Tissue Type:Hypothalmus	Treatment:HFD | Tissue Type:Hypothalmus	Treatment:HFD | Tissue Type:Hypothalmus	Treatment:HFD | Tissue Type:Hypothalmus	Treatment:HFD | Tissue Type:Hypothalmus	Treatment:HFD | Tissue Type:Liver	Treatment:HFD | Tissue Type:Liver	Treatment:HFD | Tissue Type:Liver	Treatment:HFD | Tissue Type:Liver	Treatment:HFD | Tissue Type:Liver	Treatment:HFD | Tissue Type:Liver	Treatment:HFD | Tissue Type:Muscle	Treatment:HFD | Tissue Type:Muscle	Treatment:HFD | Tissue Type:Muscle	Treatment:HFD | Tissue Type:Muscle	Treatment:HFD | Tissue Type:Muscle	Treatment:HFD | Tissue Type:Muscle	Treatment:HFD | Tissue Type:Plasma	Treatment:HFD | Tissue Type:Plasma	Treatment:HFD | Tissue Type:Plasma	Treatment:HFD | Tissue Type:Plasma	Treatment:HFD | Tissue Type:Plasma	Treatment:HFD | Tissue Type:Plasma	Treatment:LFD | Tissue Type:Adipose	Treatment:LFD | Tissue Type:Adipose	Treatment:LFD | Tissue Type:Adipose	Treatment:LFD | Tissue Type:Adipose	Treatment:LFD | Tissue Type:Adipose	Treatment:LFD | Tissue Type:Adipose	Treatment:LFD | Tissue Type:Hypothalmus	Treatment:LFD | Tissue Type:Hypothalmus	Treatment:LFD | Tissue Type:Hypothalmus	Treatment:LFD | Tissue Type:Hypothalmus	Treatment:LFD | Tissue Type:Hypothalmus	Treatment:LFD | Tissue Type:Hypothalmus	Treatment:LFD | Tissue Type:Liver	Treatment:LFD | Tissue Type:Liver	Treatment:LFD | Tissue Type:Liver	Treatment:LFD | Tissue Type:Liver	Treatment:LFD | Tissue Type:Liver	Treatment:LFD | Tissue Type:Liver	Treatment:LFD | Tissue Type:Muscle	Treatment:LFD | Tissue Type:Muscle	Treatment:LFD | Tissue Type:Muscle	Treatment:LFD | Tissue Type:Muscle	Treatment:LFD | Tissue Type:Muscle	Treatment:LFD | Tissue Type:Muscle	Treatment:LFD | Tissue Type:Plasma	Treatment:LFD | Tissue Type:Plasma	Treatment:LFD | Tissue Type:Plasma	Treatment:LFD | Tissue Type:Plasma	Treatment:LFD | Tissue Type:Plasma	Treatment:LFD | Tissue Type:Plasma	Treatment:SW | Tissue Type:Adipose	Treatment:SW | Tissue Type:Adipose	Treatment:SW | Tissue Type:Adipose	Treatment:SW | Tissue Type:Adipose	Treatment:SW | Tissue Type:Adipose	Treatment:SW | Tissue Type:Adipose	Treatment:SW | Tissue Type:Hypothalmus	Treatment:SW | Tissue Type:Hypothalmus	Treatment:SW | Tissue Type:Hypothalmus	Treatment:SW | Tissue Type:Hypothalmus	Treatment:SW | Tissue Type:Hypothalmus	Treatment:SW | Tissue Type:Hypothalmus	Treatment:SW | Tissue Type:Liver	Treatment:SW | Tissue Type:Liver	Treatment:SW | Tissue Type:Liver	Treatment:SW | Tissue Type:Liver	Treatment:SW | Tissue Type:Liver	Treatment:SW | Tissue Type:Liver	Treatment:SW | Tissue Type:Muscle	Treatment:SW | Tissue Type:Muscle	Treatment:SW | Tissue Type:Muscle	Treatment:SW | Tissue Type:Muscle	Treatment:SW | Tissue Type:Muscle	Treatment:SW | Tissue Type:Muscle	Treatment:SW | Tissue Type:Plasma	Treatment:SW | Tissue Type:Plasma	Treatment:SW | Tissue Type:Plasma	Treatment:SW | Tissue Type:Plasma	Treatment:SW | Tissue Type:Plasma	Treatment:SW | Tissue Type:Plasma
18:0 Sphinganine-1P																									818	841	663	573	688	1160																									879	962	732	1030	842	870																									1130	586	822	760	1040	730
18:1 Sphingosine	474	623	410	336	558	380							3490	3170	3520	4800	4530	5260	332	377	349	415	416	449.5000	33.4000	27.3000	36.4000	19.3000	30.4000	62.2000	151	184	290	196	242.5000	319							5800	5930	5510	6550	5440	6450	308	403	306.5000	532	364	354	53.4000	54.8000	21.7000	88.6000	35.6000	33.9000	3180	424	1130	494	541	1170							6210	7360	4950	8540	9370	8220	360	400	462	406	417	439	42.6000	43.7000	90.4000	20.3000	37.6000	47.2000
18:1 Sphingosine-1P													143	139	162	204	165	88.1000							968	808	811	736	817	1390													347	404	128	164	174	299							1180	1190	920	1310	1180	1060													262	250	497	283	317	231							1570	910	1130	1010	1170	1050
C14 Ceramide	79.6000	83.3000	71.2000	108	62.1000	73.7000	1700	2840	1530	3850	1960	1490	190	160	213	163	187	265	18.7000	19.5000	16.6000	25.8000	31.8000	34.9000	26.2000	10.7000	11	10.5000	7.6900	8.1200	85	98.3000	111	102	96.6500	108	3110	4280	2560	3040	2740	2850	233	269	294	383	336	330	21.8000	33.9000	34.9000	36.2000	30.6000	29.3000	9.3900	8.3800	7.7300	9.8300	8.0500	8.7000	190	82.5000	118	97.8000	89.8000	129	1060	2240	3100	3040	3010	1730	343	337	249	313	334	318	22.9000	17.2000	24.1000	19.1000	27.9000	36.9500	4.3000	3.3900	6.6300	6.7800	6.2500	8.1400
C16 Ceramide	4820	6510	2970	3935	9300	3270	63700	92700	62900	107000	69300	49300	10500	9600	12700	9720	11300	14700	556	598	540	783	827.5000	774.5000	1100	523	512	412	352	406	2050	2990	4680	3310	3405	3050	105000	197000	73700	117000	71200	92700	17400	14600	14700	16800	16200	15500	470	609	707.5000	942	601	588	375	351	355	365	331	328	13000	2950	6550	2980	2490	7160	44500	61400	76100	103000	118000	67800	16900	17500	16100	17200	18000	15000	653	692	1070	665	801	784.5000	241	306	322	253	305	489
C18:1 Ceramide	849	802	544	611.5000	1840	628	457000	534000	269000	611000	510000	213000	98.6000	138	137	84.6000	136	193	996	819	1010	882	929.5000	949.5000	8.3300	4.7300	5	3.7700	2.8900	5.3800	174	219	351	220	241	230.5000	616000	961000	403000	661000	471000	591000	151	122	127	120	102	99.1000	523	754	768	762	646	598	3.0600	3.2600	2.3500	3.0500	3.1900	3.2100	436	257	343	307	262	343	177000	371000	469000	510000	655000	476000	154	126	106	180	138	134	975	712	1090	783	639	969.5000	2.6600	2.7000	4.1500	3.3000	3.2700	5.3600
C18 Ceramide	2510	1790	1850	2740	3860	1920	1890000	1800000	2280000	1350000	1770000	1490000	1190	1400	1360	1060	1710	2070	6820	7510	7730	11000	11645	10795	113	48.3000	41.2000	28.1000	23.4000	40.3000	588	674	717	699	783.5000	873	1920000	3720000	1090000	2360000	1450000	2050000	1340	1600	1860	1680	1480	1630	6360	8510	11215	9360	7760	7490	28	34.8000	30.8000	31.3000	29.3000	26.9000	1860	663	939	1070	973	1230	1450000	1100000	1300000	1780000	3110000	2330000	1960	2050	1430	2970	1880	1670	12000	6270	12700	9350	9010	14500	23.8000	39.3000	46.6000	28	32.9000	69.5000
C18 dihydroceramide	87.1000	63.8000	60	70.3000	111	107	15400	12100	21400	7150	13400	14800	10.2000	14.2000	13.8000	8.0800	12	16.1000	29.6000	35.6000	52.3000	89.1000	81.5000	123	32.5000	20.1000	11	21.6000	11.8000	11.4000	21.4000	42.6000	23.4000	20.5000	76.8000	38.3500	12100	25700	6090	15200	9490	15300	52.9000	15.4000	63.5000	30.2000	16	15.8000	43.7000	32.2000	96.2500	225	55.3000	47.8000	3.4400	4.2700	6.0700	4.9600	3.5000	3.5500	75	25.3000	43.3000	29.9000	31.3000	43.3000	13500	6520	7060	12400	23800	17100	25.1000	35.1000	4.2000	57.6000	11	16.4000	61.5000	56.2000	138	79.4000	67.7000	59.5500	3.1500	4.1200	3.4400	2.1900	3.7800	5.4800
C20 Ceramide	738	1170	688	781	1040	670	109000	123000	132000	84400	115000	106000	5050	6550	6650	5380	6250	5350	68.9000	113	124	92.8000	108.0500	73.5500	406	110	75.6000	39.5000	38.2000	105	620	550	543	574	552.5000	513	125000	224000	76400	140000	93100	141000	1720	2730	2310	2390	3210	3020	100	91.3000	73.6000	157	118	125	43.2000	50.4000	50.2000	46.3000	47.3000	42.8000	1290	479	595	303	479	743	62300	74200	84000	112000	172000	141000	3110	3130	6080	2800	4020	3340	109	98.3000	185	92.7000	117	90.1000	54	67.4000	49.5000	44.1000	52.9000	68
C24 Ceramide	284	409	302	463.5000	1690	290	12800	14100	16000	11500	10600	10600	7380	7920	9460	13500	7620	7830	72.3000	142	151	297	317	109	760	61.2000	12.5000	79.6000	46.6000	94.3000	473	349	301	375	324	276.5000	16200	27700	8530	18800	11500	13700	7720	9790	8700	9880	11500	12000	264	110	154.6000	350	208	223	71.5000	61.6000	83.9000	50.5000	94	36.2000	5480	347	652	374	309	1090	7780	8220	6700	14400	17400	16000	8980	11400	15700	9890	11900	12200	235	169	500	315	385	331	142	179	28.7000	45.9000	78.2000	37.9000
C24 dihydroceramide	11.3000	14.8000	10.8000	7.8300	15.7000	11.4000							427	423	417	439	257	282	2.4500	3.8000	2.4700	7.8000	6.3950	4.5850	79.3000			8.8000			14.3000	10.6000	11.8000	12.7000	6.9000	5.5150							358	413	669	514	480	756	4.3100	7	5.9700	5.9500	3.7600	3.8600							83.5000	10.5000	17.3000	10.6000	7.6400	16.8000							294	605	680	468	352	540	3.9300	4.3200	7.9600	2.1700	6.6300	6.4400
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	moverz_quant	ri	ri_type	pubchem_id	inchi_key	kegg_id	other_id	other_id_type
18:0 Sphinganine-1P				44317142				UCDavis_Newman_ID
18:1 Sphingosine				5353955				UCDavis_Newman_ID
18:1 Sphingosine-1P				5353955				UCDavis_Newman_ID
C14 Ceramide				5282310				UCDavis_Newman_ID
C16 Ceramide				5283564				UCDavis_Newman_ID
C18:1 Ceramide				5283563				UCDavis_Newman_ID
C18 Ceramide				13846207				UCDavis_Newman_ID
C18 dihydroceramide				5283573				UCDavis_Newman_ID
C20 Ceramide				5283566				UCDavis_Newman_ID
C24 Ceramide				5283571				UCDavis_Newman_ID
C24 dihydroceramide				5283577				UCDavis_Newman_ID
METABOLITES_END
#END