#METABOLOMICS WORKBENCH mlafrano_20150924_9047701_mwtab.txt DATATRACK_ID:384 STUDY_ID:ST000257 ANALYSIS_ID:AN000407 PROJECT_ID:PR000209
VERSION             	1
CREATED_ON          	2016-09-17
#PROJECT
PR:PROJECT_TITLE                 	Metabolite changes associated with weight loss
PR:PROJECT_TYPE                  	Feeding study
PR:PROJECT_SUMMARY               	This study aims to identify changes in metabolites that occur in metabolic
PR:PROJECT_SUMMARY               	including liver, adipose (epididymal), hypothalamus, and muscle, as well as
PR:PROJECT_SUMMARY               	in the obese state that are long-lasting and not reversed by weight loss. Mice
PR:PROJECT_SUMMARY               	divided into three groups termed LFD (low fat diet), HFD (high fat diet), and
PR:PROJECT_SUMMARY               	(switch diet from HFD to LFD)(n = 6 per group). All mice were fed normal chow
PR:PROJECT_SUMMARY               	12 weeks of age. The LFD group were fed a low fat diet (10% fat) ad libitum for
PR:PROJECT_SUMMARY               	weeks. The HFD diet group were fed high fat diet (60% fat) ad libitum for 18
PR:PROJECT_SUMMARY               	The SW group were fed high fat diet for 9 weeks, then low fat diet for 9 weeks
PR:PROJECT_SUMMARY               	which their body weights returned to similar levels as the LFD group. Each
PR:PROJECT_SUMMARY               	was analyzed for metabolite and gene expression changes.
PR:INSTITUTE                     	University of California, San Diego
PR:DEPARTMENT                    	Department of Medicine
PR:LABORATORY                    	Osborn
PR:LAST_NAME                     	Osborn
PR:FIRST_NAME                    	Olivia
PR:ADDRESS                       	9500 Gilman Dr., La Jolla, CA 92093
PR:EMAIL                         	oosborn@ucsd.edu
PR:PHONE                         	+1-858-822-6645
PR:FUNDING_SOURCE                	National Institute of Health U24 DK092993
#STUDY
ST:STUDY_TITLE                   	NIH WCMC Pilot & Feasibility Project: “Metabolite changes associated with
ST:STUDY_TITLE                   	loss”
ST:STUDY_TYPE                    	Feeding study
ST:STUDY_SUMMARY                 	Targeted metabolomic analyses of oxylipins, endocannabinoids, and ceramides
ST:STUDY_SUMMARY                 	performed on 18 mouse liver, adipose, hypothalamus, plasma, and muscle samples
ST:STUDY_SUMMARY                 	from mice euthanized at 18 weeks following consumption of three diet regimens
ST:STUDY_SUMMARY                 	induced lean, obese, and weight loss phenotypes. Samples were analyzed by
ST:STUDY_SUMMARY                 	using a Waters Acquity UPLC and detected on an API 4000 QTrap (AB Sciex,
ST:STUDY_SUMMARY                 	MA, USA) by multiple reaction monitoring (MRM) after negative mode electrospray
ST:INSTITUTE                     	University of California, Davis
ST:DEPARTMENT                    	Nutrition
ST:LABORATORY                    	Newman Lab
ST:LAST_NAME                     	Newman
ST:FIRST_NAME                    	John
ST:ADDRESS                       	430 W. Health Sciences Dr., Davis, CA 95616
ST:EMAIL                         	john.newman@ars.usda.gov
ST:PHONE                         	+1-530-752-1009
ST:NUM_GROUPS                    	3
ST:TOTAL_SUBJECTS                	18
#SUBJECT
SU:SUBJECT_TYPE                  	Animal
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
SU:AGE_OR_AGE_RANGE              	30 weeks
SU:GENDER                        	Female
SU:ANIMAL_HOUSING                	Singly housed
SU:ANIMAL_FEED                   	High fat diet (D12492, Research Diets, New Brunswick, NJ), Low fat diet
SU:ANIMAL_FEED                   	Research Diets, New Brunswick, NJ)
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	-	HF epi 1	Treatment:HFD | Tissue Type:Adipose	
SUBJECT_SAMPLE_FACTORS           	-	HF epi 2	Treatment:HFD | Tissue Type:Adipose	
SUBJECT_SAMPLE_FACTORS           	-	HF epi 3	Treatment:HFD | Tissue Type:Adipose	
SUBJECT_SAMPLE_FACTORS           	-	HF epi 4	Treatment:HFD | Tissue Type:Adipose	
SUBJECT_SAMPLE_FACTORS           	-	HF epi 7	Treatment:HFD | Tissue Type:Adipose	
SUBJECT_SAMPLE_FACTORS           	-	HF epi 8	Treatment:HFD | Tissue Type:Adipose	
SUBJECT_SAMPLE_FACTORS           	-	HF Hypo 1	Treatment:HFD | Tissue Type:Hypothalmus	
SUBJECT_SAMPLE_FACTORS           	-	HF Hypo 2	Treatment:HFD | Tissue Type:Hypothalmus	
SUBJECT_SAMPLE_FACTORS           	-	HF Hypo 3	Treatment:HFD | Tissue Type:Hypothalmus	
SUBJECT_SAMPLE_FACTORS           	-	HF Hypo 4	Treatment:HFD | Tissue Type:Hypothalmus	
SUBJECT_SAMPLE_FACTORS           	-	HF Hypo 7	Treatment:HFD | Tissue Type:Hypothalmus	
SUBJECT_SAMPLE_FACTORS           	-	HF Hypo 8	Treatment:HFD | Tissue Type:Hypothalmus	
SUBJECT_SAMPLE_FACTORS           	-	HF liv 1	Treatment:HFD | Tissue Type:Liver	
SUBJECT_SAMPLE_FACTORS           	-	HF liv 2	Treatment:HFD | Tissue Type:Liver	
SUBJECT_SAMPLE_FACTORS           	-	HF liv 3	Treatment:HFD | Tissue Type:Liver	
SUBJECT_SAMPLE_FACTORS           	-	HF liv 4	Treatment:HFD | Tissue Type:Liver	
SUBJECT_SAMPLE_FACTORS           	-	HF liv 7	Treatment:HFD | Tissue Type:Liver	
SUBJECT_SAMPLE_FACTORS           	-	HF liv 8	Treatment:HFD | Tissue Type:Liver	
SUBJECT_SAMPLE_FACTORS           	-	HF mus 1	Treatment:HFD | Tissue Type:Muscle	
SUBJECT_SAMPLE_FACTORS           	-	HF mus 2	Treatment:HFD | Tissue Type:Muscle	
SUBJECT_SAMPLE_FACTORS           	-	HF mus 3	Treatment:HFD | Tissue Type:Muscle	
SUBJECT_SAMPLE_FACTORS           	-	HF mus 4	Treatment:HFD | Tissue Type:Muscle	
SUBJECT_SAMPLE_FACTORS           	-	HF mus 7	Treatment:HFD | Tissue Type:Muscle	
SUBJECT_SAMPLE_FACTORS           	-	HF mus 8	Treatment:HFD | Tissue Type:Muscle	
SUBJECT_SAMPLE_FACTORS           	-	HF plasma 1	Treatment:HFD | Tissue Type:Plasma	
SUBJECT_SAMPLE_FACTORS           	-	HF plasma 2	Treatment:HFD | Tissue Type:Plasma	
SUBJECT_SAMPLE_FACTORS           	-	HF plasma 3	Treatment:HFD | Tissue Type:Plasma	
SUBJECT_SAMPLE_FACTORS           	-	HF plasma 4	Treatment:HFD | Tissue Type:Plasma	
SUBJECT_SAMPLE_FACTORS           	-	HF plasma 7	Treatment:HFD | Tissue Type:Plasma	
SUBJECT_SAMPLE_FACTORS           	-	HF plasma 8	Treatment:HFD | Tissue Type:Plasma	
SUBJECT_SAMPLE_FACTORS           	-	LF epi 1	Treatment:LFD | Tissue Type:Adipose	
SUBJECT_SAMPLE_FACTORS           	-	LF epi 2	Treatment:LFD | Tissue Type:Adipose	
SUBJECT_SAMPLE_FACTORS           	-	LF epi 3	Treatment:LFD | Tissue Type:Adipose	
SUBJECT_SAMPLE_FACTORS           	-	LF epi 6	Treatment:LFD | Tissue Type:Adipose	
SUBJECT_SAMPLE_FACTORS           	-	LF epi 7	Treatment:LFD | Tissue Type:Adipose	
SUBJECT_SAMPLE_FACTORS           	-	LF epi 8	Treatment:LFD | Tissue Type:Adipose	
SUBJECT_SAMPLE_FACTORS           	-	LF Hypo 1	Treatment:LFD | Tissue Type:Hypothalmus	
SUBJECT_SAMPLE_FACTORS           	-	LF Hypo 2	Treatment:LFD | Tissue Type:Hypothalmus	
SUBJECT_SAMPLE_FACTORS           	-	LF Hypo 3	Treatment:LFD | Tissue Type:Hypothalmus	
SUBJECT_SAMPLE_FACTORS           	-	LF Hypo 6	Treatment:LFD | Tissue Type:Hypothalmus	
SUBJECT_SAMPLE_FACTORS           	-	LF Hypo 7	Treatment:LFD | Tissue Type:Hypothalmus	
SUBJECT_SAMPLE_FACTORS           	-	LF Hypo 8	Treatment:LFD | Tissue Type:Hypothalmus	
SUBJECT_SAMPLE_FACTORS           	-	LF liv 1	Treatment:LFD | Tissue Type:Liver	
SUBJECT_SAMPLE_FACTORS           	-	LF liv 2	Treatment:LFD | Tissue Type:Liver	
SUBJECT_SAMPLE_FACTORS           	-	LF liv 3	Treatment:LFD | Tissue Type:Liver	
SUBJECT_SAMPLE_FACTORS           	-	LF liv 6	Treatment:LFD | Tissue Type:Liver	
SUBJECT_SAMPLE_FACTORS           	-	LF liv 7	Treatment:LFD | Tissue Type:Liver	
SUBJECT_SAMPLE_FACTORS           	-	LF liv 8	Treatment:LFD | Tissue Type:Liver	
SUBJECT_SAMPLE_FACTORS           	-	LF mus 1	Treatment:LFD | Tissue Type:Muscle	
SUBJECT_SAMPLE_FACTORS           	-	LF mus 2	Treatment:LFD | Tissue Type:Muscle	
SUBJECT_SAMPLE_FACTORS           	-	LF mus 3	Treatment:LFD | Tissue Type:Muscle	
SUBJECT_SAMPLE_FACTORS           	-	LF mus 6	Treatment:LFD | Tissue Type:Muscle	
SUBJECT_SAMPLE_FACTORS           	-	LF mus 7	Treatment:LFD | Tissue Type:Muscle	
SUBJECT_SAMPLE_FACTORS           	-	LF mus 8	Treatment:LFD | Tissue Type:Muscle	
SUBJECT_SAMPLE_FACTORS           	-	LF plasma 1	Treatment:LFD | Tissue Type:Plasma	
SUBJECT_SAMPLE_FACTORS           	-	LF plasma 2	Treatment:LFD | Tissue Type:Plasma	
SUBJECT_SAMPLE_FACTORS           	-	LF plasma 3	Treatment:LFD | Tissue Type:Plasma	
SUBJECT_SAMPLE_FACTORS           	-	LF plasma 6	Treatment:LFD | Tissue Type:Plasma	
SUBJECT_SAMPLE_FACTORS           	-	LF plasma 7	Treatment:LFD | Tissue Type:Plasma	
SUBJECT_SAMPLE_FACTORS           	-	LF plasma 8	Treatment:LFD | Tissue Type:Plasma	
SUBJECT_SAMPLE_FACTORS           	-	SW epi 2	Treatment:SW | Tissue Type:Adipose	
SUBJECT_SAMPLE_FACTORS           	-	SW epi 3	Treatment:SW | Tissue Type:Adipose	
SUBJECT_SAMPLE_FACTORS           	-	SW epi 4	Treatment:SW | Tissue Type:Adipose	
SUBJECT_SAMPLE_FACTORS           	-	SW epi 5	Treatment:SW | Tissue Type:Adipose	
SUBJECT_SAMPLE_FACTORS           	-	SW epi 7	Treatment:SW | Tissue Type:Adipose	
SUBJECT_SAMPLE_FACTORS           	-	SW epi 8	Treatment:SW | Tissue Type:Adipose	
SUBJECT_SAMPLE_FACTORS           	-	SW Hypo 2	Treatment:SW | Tissue Type:Hypothalmus	
SUBJECT_SAMPLE_FACTORS           	-	SW Hypo 3	Treatment:SW | Tissue Type:Hypothalmus	
SUBJECT_SAMPLE_FACTORS           	-	SW Hypo 4	Treatment:SW | Tissue Type:Hypothalmus	
SUBJECT_SAMPLE_FACTORS           	-	SW Hypo 5	Treatment:SW | Tissue Type:Hypothalmus	
SUBJECT_SAMPLE_FACTORS           	-	SW Hypo 7	Treatment:SW | Tissue Type:Hypothalmus	
SUBJECT_SAMPLE_FACTORS           	-	SW Hypo 8	Treatment:SW | Tissue Type:Hypothalmus	
SUBJECT_SAMPLE_FACTORS           	-	SW liv 2	Treatment:SW | Tissue Type:Liver	
SUBJECT_SAMPLE_FACTORS           	-	SW liv 3	Treatment:SW | Tissue Type:Liver	
SUBJECT_SAMPLE_FACTORS           	-	SW liv 4	Treatment:SW | Tissue Type:Liver	
SUBJECT_SAMPLE_FACTORS           	-	SW liv 5	Treatment:SW | Tissue Type:Liver	
SUBJECT_SAMPLE_FACTORS           	-	SW liv 7	Treatment:SW | Tissue Type:Liver	
SUBJECT_SAMPLE_FACTORS           	-	SW liv 8	Treatment:SW | Tissue Type:Liver	
SUBJECT_SAMPLE_FACTORS           	-	SW mus 2	Treatment:SW | Tissue Type:Muscle	
SUBJECT_SAMPLE_FACTORS           	-	SW mus 3	Treatment:SW | Tissue Type:Muscle	
SUBJECT_SAMPLE_FACTORS           	-	SW mus 4	Treatment:SW | Tissue Type:Muscle	
SUBJECT_SAMPLE_FACTORS           	-	SW mus 5	Treatment:SW | Tissue Type:Muscle	
SUBJECT_SAMPLE_FACTORS           	-	SW mus 7	Treatment:SW | Tissue Type:Muscle	
SUBJECT_SAMPLE_FACTORS           	-	SW mus 8	Treatment:SW | Tissue Type:Muscle	
SUBJECT_SAMPLE_FACTORS           	-	SW plasma 2	Treatment:SW | Tissue Type:Plasma	
SUBJECT_SAMPLE_FACTORS           	-	SW plasma 3	Treatment:SW | Tissue Type:Plasma	
SUBJECT_SAMPLE_FACTORS           	-	SW plasma 4	Treatment:SW | Tissue Type:Plasma	
SUBJECT_SAMPLE_FACTORS           	-	SW plasma 5	Treatment:SW | Tissue Type:Plasma	
SUBJECT_SAMPLE_FACTORS           	-	SW plasma 7	Treatment:SW | Tissue Type:Plasma	
SUBJECT_SAMPLE_FACTORS           	-	SW plasma 8	Treatment:SW | Tissue Type:Plasma	
#COLLECTION
CO:COLLECTION_SUMMARY            	-
CO:COLLECTION_PROTOCOL_FILENAME  	NewmanLab-Mediators-Protocol-PT.pdf
CO:SAMPLE_TYPE                   	Liver, adipose, hypothalamus, muscle, and plasma
CO:COLLECTION_METHOD             	Dissection
CO:STORAGE_CONDITIONS            	- 80°C
CO:STORAGE_VIALS                 	Eppendorf tubes
#TREATMENT
TR:TREATMENT_SUMMARY             	Mice were fed 18 weeks on either a low fat diet (LF), high fat diet (HF), or
TR:TREATMENT_SUMMARY             	of both (SW).
TR:TREATMENT_PROTOCOL_FILENAME   	NewmanLab-Mediators-Protocol-PT.pdf
TR:TREATMENT                     	Intervention
TR:TREATMENT_DOSEDURATION        	18 weeks
TR:TREATMENT_VEHICLE             	animal feed
TR:ANIMAL_ENDP_EUTHANASIA        	30 weeks
TR:ANIMAL_ENDP_TISSUE_COLL_LIST  	Liver, adipose, hypothalami, muscle, and plasma
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Tissues were pulverized on dry ice, enriched with deuterated surrogates,
SP:SAMPLEPREP_SUMMARY            	(BHT and EDTA), and extracted with MeOH:Water:IPA, transferred to an Ostro
SP:SAMPLEPREP_SUMMARY            	and the extract was eluted by vacuum. Plasma was added directly to the plate,
SP:SAMPLEPREP_SUMMARY            	with the surrogates, antioxidants, and IPA and the extract eluted through. The
SP:SAMPLEPREP_SUMMARY            	were dried, reconstituted in 100?L 50:50 methanol:acetonitrile with internal
SP:SAMPLEPREP_SUMMARY            	1?phenyl?3?hexanoic acid urea and 1?cyclohexyl?3-dodecanoic acid urea
SP:SAMPLEPREP_SUMMARY            	St. Louis, MO) and filtered at 0.1 µm.
SP:SAMPLEPREP_PROTOCOL_FILENAME  	NewmanLab-Mediators-Protocol-PT.pdf
SP:EXTRACTION_METHOD             	Ostro plate
SP:EXTRACT_CLEANUP               	Ostro plate
SP:EXTRACT_STORAGE               	- 20 °C
SP:SAMPLE_RESUSPENSION           	100 µL
SP:SAMPLE_SPIKING                	See sample prep protocol file
SP:ORGAN                         	Liver, adipose, hypothalamus, muscle, and plasma
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	Endocannabinoid analysis
CH:CHROMATOGRAPHY_TYPE           	-
CH:INSTRUMENT_NAME               	Waters Acquity
CH:COLUMN_NAME                   	-
CH:METHODS_FILENAME              	NewmanLab-Mediators-Protocol-PT.pdf
CH:COLUMN_TEMPERATURE            	60 C
CH:FLOW_GRADIENT                 	See protocol/methods file
CH:FLOW_RATE                     	0.25
CH:INTERNAL_STANDARD             	See protocol/methods file
CH:RETENTION_TIME                	See protocol/methods file
CH:SAMPLE_INJECTION              	5 L
CH:SOLVENT_A                     	0.1% acetic acid
CH:SOLVENT_B                     	90% ACN / 10% IPA
CH:ANALYTICAL_TIME               	11 min
CH:WEAK_WASH_SOLVENT_NAME        	20% methanol, 10% isopropanol
CH:WEAK_WASH_VOLUME              	600 L
CH:STRONG_WASH_SOLVENT_NAME      	50:50 Acetonitrile:Methanol
CH:STRONG_WASH_VOLUME            	600 L
CH:SAMPLE_LOOP_SIZE              	17 L
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
AN:LABORATORY_NAME               	Newman
AN:SOFTWARE_VERSION              	AB Sciex MultiQuant version 3.0.1
AN:OPERATOR_NAME                 	Michael La Frano
AN:DETECTOR_TYPE                 	API 4000 QTrap (AB Sciex, Framingham, MA, USA)
AN:DATA_FORMAT                   	.mzML
#MS
MS:INSTRUMENT_NAME               	ABI Sciex API 4000 QTrap
MS:INSTRUMENT_TYPE               	Triple quadrupole
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:CAPILLARY_TEMPERATURE         	See protocol file
MS:CAPILLARY_VOLTAGE             	Positive
MS:MS_COMMENTS                   	Endocannabinoid analysis
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS         	nM
MS_METABOLITE_DATA_START
Samples	HF epi 1	HF epi 2	HF epi 3	HF epi 4	HF epi 7	HF epi 8	HF Hypo 1	HF Hypo 2	HF Hypo 3	HF Hypo 4	HF Hypo 7	HF Hypo 8	HF liv 1	HF liv 2	HF liv 3	HF liv 4	HF liv 7	HF liv 8	HF mus 1	HF mus 2	HF mus 3	HF mus 4	HF mus 7	HF mus 8	HF plasma 1	HF plasma 2	HF plasma 3	HF plasma 4	HF plasma 7	HF plasma 8	LF epi 1	LF epi 2	LF epi 3	LF epi 6	LF epi 7	LF epi 8	LF Hypo 1	LF Hypo 2	LF Hypo 3	LF Hypo 6	LF Hypo 7	LF Hypo 8	LF liv 1	LF liv 2	LF liv 3	LF liv 6	LF liv 7	LF liv 8	LF mus 1	LF mus 2	LF mus 3	LF mus 6	LF mus 7	LF mus 8	LF plasma 1	LF plasma 2	LF plasma 3	LF plasma 6	LF plasma 7	LF plasma 8	SW epi 2	SW epi 3	SW epi 4	SW epi 5	SW epi 7	SW epi 8	SW Hypo 2	SW Hypo 3	SW Hypo 4	SW Hypo 5	SW Hypo 7	SW Hypo 8	SW liv 2	SW liv 3	SW liv 4	SW liv 5	SW liv 7	SW liv 8	SW mus 2	SW mus 3	SW mus 4	SW mus 5	SW mus 7	SW mus 8	SW plasma 2	SW plasma 3	SW plasma 4	SW plasma 5	SW plasma 7	SW plasma 8
Factors	Treatment:HFD | Tissue Type:Adipose	Treatment:HFD | Tissue Type:Adipose	Treatment:HFD | Tissue Type:Adipose	Treatment:HFD | Tissue Type:Adipose	Treatment:HFD | Tissue Type:Adipose	Treatment:HFD | Tissue Type:Adipose	Treatment:HFD | Tissue Type:Hypothalmus	Treatment:HFD | Tissue Type:Hypothalmus	Treatment:HFD | Tissue Type:Hypothalmus	Treatment:HFD | Tissue Type:Hypothalmus	Treatment:HFD | Tissue Type:Hypothalmus	Treatment:HFD | Tissue Type:Hypothalmus	Treatment:HFD | Tissue Type:Liver	Treatment:HFD | Tissue Type:Liver	Treatment:HFD | Tissue Type:Liver	Treatment:HFD | Tissue Type:Liver	Treatment:HFD | Tissue Type:Liver	Treatment:HFD | Tissue Type:Liver	Treatment:HFD | Tissue Type:Muscle	Treatment:HFD | Tissue Type:Muscle	Treatment:HFD | Tissue Type:Muscle	Treatment:HFD | Tissue Type:Muscle	Treatment:HFD | Tissue Type:Muscle	Treatment:HFD | Tissue Type:Muscle	Treatment:HFD | Tissue Type:Plasma	Treatment:HFD | Tissue Type:Plasma	Treatment:HFD | Tissue Type:Plasma	Treatment:HFD | Tissue Type:Plasma	Treatment:HFD | Tissue Type:Plasma	Treatment:HFD | Tissue Type:Plasma	Treatment:LFD | Tissue Type:Adipose	Treatment:LFD | Tissue Type:Adipose	Treatment:LFD | Tissue Type:Adipose	Treatment:LFD | Tissue Type:Adipose	Treatment:LFD | Tissue Type:Adipose	Treatment:LFD | Tissue Type:Adipose	Treatment:LFD | Tissue Type:Hypothalmus	Treatment:LFD | Tissue Type:Hypothalmus	Treatment:LFD | Tissue Type:Hypothalmus	Treatment:LFD | Tissue Type:Hypothalmus	Treatment:LFD | Tissue Type:Hypothalmus	Treatment:LFD | Tissue Type:Hypothalmus	Treatment:LFD | Tissue Type:Liver	Treatment:LFD | Tissue Type:Liver	Treatment:LFD | Tissue Type:Liver	Treatment:LFD | Tissue Type:Liver	Treatment:LFD | Tissue Type:Liver	Treatment:LFD | Tissue Type:Liver	Treatment:LFD | Tissue Type:Muscle	Treatment:LFD | Tissue Type:Muscle	Treatment:LFD | Tissue Type:Muscle	Treatment:LFD | Tissue Type:Muscle	Treatment:LFD | Tissue Type:Muscle	Treatment:LFD | Tissue Type:Muscle	Treatment:LFD | Tissue Type:Plasma	Treatment:LFD | Tissue Type:Plasma	Treatment:LFD | Tissue Type:Plasma	Treatment:LFD | Tissue Type:Plasma	Treatment:LFD | Tissue Type:Plasma	Treatment:LFD | Tissue Type:Plasma	Treatment:SW | Tissue Type:Adipose	Treatment:SW | Tissue Type:Adipose	Treatment:SW | Tissue Type:Adipose	Treatment:SW | Tissue Type:Adipose	Treatment:SW | Tissue Type:Adipose	Treatment:SW | Tissue Type:Adipose	Treatment:SW | Tissue Type:Hypothalmus	Treatment:SW | Tissue Type:Hypothalmus	Treatment:SW | Tissue Type:Hypothalmus	Treatment:SW | Tissue Type:Hypothalmus	Treatment:SW | Tissue Type:Hypothalmus	Treatment:SW | Tissue Type:Hypothalmus	Treatment:SW | Tissue Type:Liver	Treatment:SW | Tissue Type:Liver	Treatment:SW | Tissue Type:Liver	Treatment:SW | Tissue Type:Liver	Treatment:SW | Tissue Type:Liver	Treatment:SW | Tissue Type:Liver	Treatment:SW | Tissue Type:Muscle	Treatment:SW | Tissue Type:Muscle	Treatment:SW | Tissue Type:Muscle	Treatment:SW | Tissue Type:Muscle	Treatment:SW | Tissue Type:Muscle	Treatment:SW | Tissue Type:Muscle	Treatment:SW | Tissue Type:Plasma	Treatment:SW | Tissue Type:Plasma	Treatment:SW | Tissue Type:Plasma	Treatment:SW | Tissue Type:Plasma	Treatment:SW | Tissue Type:Plasma	Treatment:SW | Tissue Type:Plasma
11(12)-EpETre EA																																																																																										
15-HETE EA																																																																																										
1-AG	66.1000	157	25.2000	59.2000	98.6000	46.5000	6650	2700	15000	3630	3940	5670	5370	1530	954	1590	2690	906	173	215	220	231	219.5000	217	115	90.9000	67.5000	198	127	92.3000	27.1000	23.1000	51.9000	20.5000	47.6500	51.3000	6040	11700	3700	4770	4040	5070	478	2300	2750	4030	5960	5310	303	234	244.5000	327	287	202	95.9000	61	69.7000	73.5000	81.5000	132	578	37.4000	162	54.2000	130	181	7580	4430	4230	3650	5090	7010	2800	4140	2770	3860	1610	2230	285	257	253	190	475	250.5000	128	101	86.4000	132	115	96.5000
1-LG	2150	1920	442	614	787	907	2610	1210	2740	1420	1050	2190	19000	4940	3000	8350	10500	3740	483	287	331	701	400	392.5000	3070	2960	1580	5540	5110	3790	190	175	375	203	456	539.5000	1290	2830	585	1090	654	1150	777	16500	4920	8380	29500	15500	558	354	459	751	421	289	1870	1270	1470	895	1880	3530	8450	826	1750	1200	973	2060	3330	881	1000	1540	1050	1510	9400	9590	6360	9630	4620	4950	936	773	1100	299	359	468	3820	1440	1080	3070	2340	1270
1-OG	7720	8390	1730	2830	4100	3010	50400	42400	59300	34400	33600	41700	28300	12300	15300	12800	23100	18900	1220	1290	919	1840	1201	1750	8820	4230	1750	7110	7460	4350	946	1110	1960	1140	2170	2080	42500	97400	29000	53000	39500	61700	15600	78000	41600	52900	123000	85500	1510	945	1614	2610	1330	940	8630	5510	5040	4550	6180	8040	47800	3850	6080	3980	3190	9700	37600	49000	40700	42800	41200	67300	65300	61800	37600	55800	46500	30700	2420	1920	3950	946	1370	1590	11400	8200	4240	7520	8520	5850
2-AG	116	544	43.7000	143.5000	276	215	31800	9200	79500	14400	13700	19500	25300	7600	3410	10700	12500	1840	649	759	812	661	978.5000	759.5000	225	135	194	332	155	221	58.7000	41	118	33.7000	118.5500	97.0500	25700	59100	15000	15700	15100	24300	1470	21000	12300	17300	34100	27900	855	942	676.5000	892	1060	880	118	116	113	78	159	322	1150	131	899	228	470	822	28000	17800	19000	14800	16400	34600	13900	23500	15700	16700	4990	11100	902	800	928	756	1490	894	206	95.6000	132	285	246	147
2-LG	5190	10100	1750	2350	2560	3700	14000	3880	13600	7550	4910	8950	200000	71300	36300	118000	102000	30900	1890	2100	1030	2730	1166	1445	12900	6790	5640	12400	9190	12300	891	725	1620	786	2030	1557.5000	5650	10600	2800	5010	1930	3920	10700	168000	49200	83200	268000	158000	1960	1190	1465	2700	1920	1300	2900	2330	2800	1760	3920	7740	20900	3650	8370	5730	5680	13500	13200	4410	5070	6800	2500	6540	94400	114000	79300	81800	42200	52400	3930	4150	4980	1370	2390	2510	8510	2570	2020	6920	5300	2500
2-OG	9780	19200	2420	5555	3530	5990	265000	167000	361000	155000	171000	229000	193000	63300	66500	85000	133000	54300	1990	2940	1770	3950	2595	2940	17000	7130	5210	10300	12900	10500	1720	1120	3750	1780	4120	3595	268000	523000	183000	216000	228000	314000	79300	511000	206000	237000	638000	322000	3400	2230	2560	5540	3230	2190	11600	8890	7860	7370	9960	17500	53400	5480	18700	10500	11200	30400	209000	282000	245000	226000	209000	464000	264000	281000	172000	335000	195000	167000	5050	6060	8060	1920	4090	4170	20200	12500	8250	15800	15500	11400
AEA	12.7000	13.4000	15.2000	25.8000	18.7000	13.8000	205	224	231	196	180	162	3.8900	4.9900	4.0800	4.4400	3.0600	5.7900	4.7800	6.5000	6.6600	7.8700	5.9600	6.7850	3.4300	3.3400	2.9000	4.3600	3.4200	3.3400	20.2000	20.6000	54.3000	14.5000	19.3500	20.8500	206	301	152	232	197	234	6.9600	4.5300	3.3300	2.4900	3.6300	4.4300	5.6800	4.8400	3.9250	8.7500	6.7400	7.3900	3.2200	3.0200	2.2100	2.9200	3.5100	3.4900	29.7000	21	20.4000	17.5000	16.6000	23.4000	202	153	161	264	366	270	3.6100	3.5900	5.9500	2.9000	6.0600	2.2800	5.4900	9.2500	8.1300	8.6600	5.3100	5.9000	3.1100	2.3700	2.3900	2.3200	2.8800	2.1700
aLEA	2.2300	2.3700	2.2700	3.8400	3.6800	2.2400							0.4720	0.4530	0.4700	0.5930	0.2390	0.4730	0.7420	0.9140	1.5800	1.2900	0.7440	0.6995							1.8800	1.8000	2.3300	1.9700	2.2300	2.1800							0.4570	1.3000	0.1350	0.1060	0.2080	0.6300	0.8750	0.6310	0.8410	1.5100	0.7570	0.9080							4.1200	1.7400	2.2700	1.5200	2.0700	2.4200							0.1310	0.2340	2.4600	0.2010	0.3270	0.2140	0.7420	1.5500	2.1400	1.9000	0.8230	0.7515						
DEA							58.6000	56.2000	84.4000	55.3000	49.1000	52.7000														0.7710	0.7380	1.0200		0.7960							49.7000	68.9000	57.7000	53	59.7000	75.5000													0.6280	0.6230	0.6920	0.7200	0.9420	1.0500							40.9000	53	66.3000	74.3000	80	83.3000													0.8370		0.7890	0.6800	0.8930	0.6660
DHEA	11.8000	9.3900	11.8000	16.1500	13.8000	10.8000	289	313	354	233	218	277	1.8500	2.7700	2.3400	3.2800	2.4900	2.8900	9.6800	12.1000	12.8000	13.1000	11.6500	13.2500	2.7400	2.7500	3.2400	4.0600	3.1000	3.6700	14.7000	13.4000	27.6000	13.1000	12.7500	13.7500	254	327	226	268	287	332	4.5100	3.0200	3.5100	2.4200	2.8700	2.3600	10.1000	13	11.5000	17.2000	14.3000	12.6000	3.0200	2.9300	2.2800	3.4200	3.2500	4.2800	31.6000	13.5000	14.9000	13.7000	12.5000	16.9000	309	189	233	318	400	366	2.7900	2.9000	4.2700	2.9700	4.9600	2.3400	13.6000	18.3000	15.6000	14.5000	11.9000	13.3500	2.9400	2.1400	2.6600	3.0200	3.4600	2.4100
Dihomo GLA EA	2.0300	2.0600	2.1100	3.1800	2.8000	2.4600		13.3000		11.9000			0.8640	0.5670	0.8370	0.8010	0.7040	1.1600	0.9190	1.6500	2.4900	1.7000	1.8200	1.6800							3.4100	4.0100	8.8400	2.4400	3.8550	3.7650	10.3000		10.2000		10.5000	17.7000	1.1700	1.9300	0.7120	0.5380	0.9870	1.3800	1.4800	1.0400	1.4300	3.2200	1.7000	1.8800							5.0100	2.6300	3.3100	2.7900	2.5100	4.1300		7.5300	12	13.9000			0.6640	0.9140	3.2600	0.8430	1.1700	0.7050	1.3500	2.3400	3.0400	3.0900	1.0100	1.5000						
LEA	164	184	166	216.5000	225	161	194	206	281	172	143	209	14.2000	16.9000	14.2000	19.2000	11	16.7000	37	47.9000	45.1000	71.5000	32.8500	36.0500	26.5000	21	18.3000	27.6000	26.1000	20.7000	95.9000	106	189	80.3000	99.9000	145.5000	93	149	77.2000	126	88.5000	116	13	9.5000	7.3800	6.6500	9.8900	11.3000	26.7000	25.3000	29.1500	52.9000	38.3000	30.6000	10.7000	8.7200	7.8400	10	12.2000	10.7000	233	120	132	120	123	139	152	62.7000	71.7000	120	141	120	7.5000	9.5300	14.7000	7.7600	12	7.2200	44.7000	75.2000	61.1000	45.6000	32.4000	32.2000	10.2000	8.7300	10.1000	11.2000	11.3000	10
NA-Gly	0.3580	0.9340	1.5700	0.5985	12.7000								34.7000	29.6000	29	22	19.3000	62	0.7230	1.0400	9.7600	1.4400	6.3500	2.8900							1.0900	3.5300	6.9600	0.3650	0.3570	0.2655							26.6000	21.9000	22.4000	8.0800	23.3000	19.6000	2.7200	2.2800	4.0600	12.5000	1.3700	4.5600							3.1900	2.9600	2.6000	2.3600	0.9460	4.0100							22.1000	13.7000	51.5000	16.1000	41.1000	11	3.5300	1.5300	30.3000	22.9000	1.4600	0.5560						
NO-Gly	6.1700	4.8800	6.0800	6.9750	26.5000	4.6800	50	55.3000	125	29.5000	34	41.8000	25.5000	26.5000	43.5000	23.1000	21.2000	105	4.7200	4.3300	21.5000	7.3200	8.0200	7.6000							3.1700	5.0800	15.5000	3.5700	3.0600	6.3050	36.1000	50	19.2000	48.5000	31	213	109	108	56.1000	40	42.4000	92.4000	7.3800	7.8600	8.5650	22.9000	7.2400	7.9500							19.8000	8.3100	7.0500	5.3900	3.0500	10.5000	58	38.9000	162	27.5000	214	50.7000	52.3000	41.5000	292	34.9000	108	26.5000	8.0800	8.7700	53.8000	64.3000	4.3400	7.3250						
OEA	246	270	291	404.5000	523	325	785	701	973	587	582	678	29.6000	21.6000	24.6000	22.3000	19.7000	22.1000	75.5000	84	118	193	151.5000	177.5000	58.5000	36.8000	34.4000	57.8000	80.5000	41.5000	171	216	363	185	275	379.5000	610	1020	555	811	665	778	39.4000	34.5000	32.8000	28.3000	32.3000	41.5000	73.6000	59	145	424	135	89.5000	24.5000	21.2000	20.2000	29.6000	33.8000	30	714	242	316	276	301	348	673	584	672	935	993	1200	33.8000	40.5000	41.4000	38.6000	39.6000	31.1000	108	191	155	193	95.4000	103.1000	28.8000	45.3000	27.8000	28.4000	30.7000	32.7000
PEA	170	192	204	286	302	265	1960	2080	3230	1040	1500	2120	109	51.6000	64.4000	43.3000	46.6000	47.2000	1180	149	832	362	682	3063.5000		24.4000	28.2000	38	62.1000	29.3000	132	272	353	172	347	511.5000	1310	2220	1100	1830	1560	2200	143	101	134	93.5000	150	120	197	157	734	1880	455	309	23.8000	20.3000	21.2000	20.6000	25.4000	36.1000	538	211	270	154	189	273	2060	1290	1180	1830	2330	3350	82.9000	111	99.5000	123	110	82.3000	1500	922	417	622	3040	1111.5000	25.9000	44.3000	27.5000	26.4000	26.8000	26.2000
PGD2 EA																																																																																										
PGE2 1G																																																																																										
PGE2 EA																																																																																										
PGF2a 1G																																																																																										
PGF2a EA																																																																																										
SEA	195	267	231	466	488	319	851	870	1520	503	680	896	133	89.1000	134	66.2000	63.4000	120	1540	168	1080	351	713	4595	70	31.9000	36.9000	50.3000	72	39.8000	130	229	540	82.8000	250.5000	230	589	1080	460	802	605	1150	116	70.9000	99	58	81	105	166	56.1000	592	2090	504	270	22.2000				21.1000	25.5000	359	172	189	140	134	229	888	498	705	775	1190	1980	90	82.5000	119	58.9000	82.4000	56.8000	1960	1200	597	961	4280	1329	22.4000		23.5000	22.1000		22.2000
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	moverz_quant	ri	ri_type	pubchem_id	inchi_key	kegg_id	other_id	other_id_type
11(12)-EpETre EA				16061183				UCDavis_Newman_ID
15-HETE EA								UCDavis_Newman_ID
1-AG				16019980				UCDavis_Newman_ID
1-LG				6436630				UCDavis_Newman_ID
1-OG				12178130				UCDavis_Newman_ID
2-AG				5282280				UCDavis_Newman_ID
2-LG				5365676				UCDavis_Newman_ID
2-OG				5319879				UCDavis_Newman_ID
AEA				5281969				UCDavis_Newman_ID
aLEA				5283449				UCDavis_Newman_ID
DEA				5282273				UCDavis_Newman_ID
DHEA				53245830				UCDavis_Newman_ID
Dihomo GLA EA				5282272				UCDavis_Newman_ID
LEA				5283446				UCDavis_Newman_ID
NA-Gly				5283389				UCDavis_Newman_ID
NO-Gly				6436908				UCDavis_Newman_ID
OEA				5283454				UCDavis_Newman_ID
PEA				4671				UCDavis_Newman_ID
PGD2 EA								UCDavis_Newman_ID
PGE2 1G				52193688				UCDavis_Newman_ID
PGE2 EA				35021785				UCDavis_Newman_ID
PGF2a 1G				24778485				UCDavis_Newman_ID
PGF2a EA				53481911				UCDavis_Newman_ID
SEA				27902				UCDavis_Newman_ID
METABOLITES_END
#END