{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002326","ANALYSIS_ID":"AN003795","VERSION":"1","CREATED_ON":"October 26, 2022, 6:28 pm"},

"PROJECT":{"PROJECT_TITLE":"Reducing the metabolic burden of rRNA synthesis promotes healthy longevity","PROJECT_SUMMARY":"Ribosome biogenesis is an anabolic process driven by RNA polymerase I (Pol I)-mediated synthesis of pre-ribosomal RNA (pre-rRNA). While Pol I activity was previously linked to longevity, the underlying mechanisms were not studied beyond effects on protein translation and downstream proteostasis. Here we used multi-omics and functional tests to show that curtailment of Pol I activity preserves mitochondrial function and lowers ATP expenditure, thereby promoting longevity in Caenorhabditis elegans. Reduced pre-rRNA synthesis also improved longevity and energy homeostasis in Drosophila melanogaster and human cells, respectively. Conversely, the enhancement of pre-rRNA synthesis boosted growth and neuromuscular performance of young nematodes at the cost of accelerated metabolic decline, mitochondrial stress and premature aging. Moreover, restriction of Pol I activity extended lifespan more potently than translational repression, and retained its geroprotective effects when initiated late in life, showcasing moderation of Pol I activity as an effective longevity treatment not limited by aging.","INSTITUTE":"University of Innsbruck","DEPARTMENT":"Michael Popp Institute","LAST_NAME":"Koeberle","FIRST_NAME":"Andreas","ADDRESS":"Mitterweg 24, Innsbruck, Tyrol, 6020, Austria","EMAIL":"andreas.koeberle@uibk.ac.at","PHONE":"+43 512 507 57903","FUNDING_SOURCE":"Leibniz Association, Thüringer Aufbaubank (2019 FGR 0082), Free State of Thuringia (RegenerAging—FSU-I-03/14), Carl-Zeiss-Stiftung (IMPULS), Phospholipid Research Center (AKO-2019-070/2-1)","CONTRIBUTORS":"Samim Sharifi, Finja Witt, André Gollowitzer, Oliver Werz, Holger Bierhoff, Maria Ermolaeva"},

"STUDY":{"STUDY_TITLE":"Analysis of age-dependent changes of triacylglycerol species in C. elegans depleted of tif-IA and ncl-1","STUDY_SUMMARY":"Analysis of triacylglycerol species in young (d2), middle age (d6) and old age (d12) C. elegans with tif-IA or ncl-1 knockdown.","INSTITUTE":"University of Innsbruck","LAST_NAME":"Koeberle","FIRST_NAME":"Andreas","ADDRESS":"Mitterweg 24, Innsbruck, Tyrol, 6020, Austria","EMAIL":"andreas.koeberle@uibk.ac.at","PHONE":"+43 512 507 57903"},

"SUBJECT":{"SUBJECT_TYPE":"Invertebrate","SUBJECT_SPECIES":"Caenorhabditis elegans","TAXONOMY_ID":"6239","AGE_OR_AGE_RANGE":"Young, middle aged and old"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva001",
"Factors":{"Treatment":"ctrl RNAi","Days of adulthood":"2"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva002",
"Factors":{"Treatment":"ctrl RNAi","Days of adulthood":"6"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva003",
"Factors":{"Treatment":"ctrl RNAi","Days of adulthood":"12"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva004",
"Factors":{"Treatment":"tif-IA RNAi","Days of adulthood":"2"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva005",
"Factors":{"Treatment":"tif-IA RNAi","Days of adulthood":"6"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva006",
"Factors":{"Treatment":"tif-IA RNAi","Days of adulthood":"12"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva007",
"Factors":{"Treatment":"ncl-1 RNAi","Days of adulthood":"2"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva008",
"Factors":{"Treatment":"ncl-1 RNAi","Days of adulthood":"6"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva009",
"Factors":{"Treatment":"ncl-1 RNAi","Days of adulthood":"12"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva010",
"Factors":{"Treatment":"ctrl RNAi","Days of adulthood":"2"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva011",
"Factors":{"Treatment":"ctrl RNAi","Days of adulthood":"6"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva012",
"Factors":{"Treatment":"ctrl RNAi","Days of adulthood":"12"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva013",
"Factors":{"Treatment":"tif-IA RNAi","Days of adulthood":"2"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva014",
"Factors":{"Treatment":"tif-IA RNAi","Days of adulthood":"6"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva015",
"Factors":{"Treatment":"tif-IA RNAi","Days of adulthood":"12"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva016",
"Factors":{"Treatment":"ncl-1 RNAi","Days of adulthood":"2"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva017",
"Factors":{"Treatment":"ncl-1 RNAi","Days of adulthood":"6"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva018",
"Factors":{"Treatment":"ncl-1 RNAi","Days of adulthood":"12"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva019",
"Factors":{"Treatment":"ctrl RNAi","Days of adulthood":"2"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva020",
"Factors":{"Treatment":"ctrl RNAi","Days of adulthood":"6"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva021",
"Factors":{"Treatment":"ctrl RNAi","Days of adulthood":"12"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva022",
"Factors":{"Treatment":"tif-IA RNAi","Days of adulthood":"2"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva023",
"Factors":{"Treatment":"tif-IA RNAi","Days of adulthood":"6"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva024",
"Factors":{"Treatment":"tif-IA RNAi","Days of adulthood":"12"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva025",
"Factors":{"Treatment":"ncl-1 RNAi","Days of adulthood":"2"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva026",
"Factors":{"Treatment":"ncl-1 RNAi","Days of adulthood":"6"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva027",
"Factors":{"Treatment":"ncl-1 RNAi","Days of adulthood":"12"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva028",
"Factors":{"Treatment":"ctrl RNAi","Days of adulthood":"2"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva029",
"Factors":{"Treatment":"ctrl RNAi","Days of adulthood":"6"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva030",
"Factors":{"Treatment":"ctrl RNAi","Days of adulthood":"12"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva031",
"Factors":{"Treatment":"tif-IA RNAi","Days of adulthood":"2"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva032",
"Factors":{"Treatment":"tif-IA RNAi","Days of adulthood":"6"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva033",
"Factors":{"Treatment":"tif-IA RNAi","Days of adulthood":"12"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva034",
"Factors":{"Treatment":"ncl-1 RNAi","Days of adulthood":"2"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva035",
"Factors":{"Treatment":"ncl-1 RNAi","Days of adulthood":"6"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva036",
"Factors":{"Treatment":"ncl-1 RNAi","Days of adulthood":"12"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva037",
"Factors":{"Treatment":"ctrl RNAi","Days of adulthood":"2"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva038",
"Factors":{"Treatment":"ctrl RNAi","Days of adulthood":"6"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva039",
"Factors":{"Treatment":"ctrl RNAi","Days of adulthood":"12"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva040",
"Factors":{"Treatment":"tif-IA RNAi","Days of adulthood":"2"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva041",
"Factors":{"Treatment":"tif-IA RNAi","Days of adulthood":"6"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva042",
"Factors":{"Treatment":"tif-IA RNAi","Days of adulthood":"12"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva043",
"Factors":{"Treatment":"ncl-1 RNAi","Days of adulthood":"2"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva044",
"Factors":{"Treatment":"ncl-1 RNAi","Days of adulthood":"6"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva045",
"Factors":{"Treatment":"ncl-1 RNAi","Days of adulthood":"12"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva001_TAGII",
"Factors":{"Treatment":"ctrl RNAi","Days of adulthood":"2"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva002_TAGII",
"Factors":{"Treatment":"ctrl RNAi","Days of adulthood":"6"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva003_TAGII",
"Factors":{"Treatment":"ctrl RNAi","Days of adulthood":"12"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva004_TAGII",
"Factors":{"Treatment":"tif-IA RNAi","Days of adulthood":"2"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva005_TAGII",
"Factors":{"Treatment":"tif-IA RNAi","Days of adulthood":"6"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva006_TAGII",
"Factors":{"Treatment":"tif-IA RNAi","Days of adulthood":"12"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva007_TAGII",
"Factors":{"Treatment":"ncl-1 RNAi","Days of adulthood":"2"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva008_TAGII",
"Factors":{"Treatment":"ncl-1 RNAi","Days of adulthood":"6"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva009_TAGII",
"Factors":{"Treatment":"ncl-1 RNAi","Days of adulthood":"12"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva010_TAGII",
"Factors":{"Treatment":"ctrl RNAi","Days of adulthood":"2"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva011_TAGII",
"Factors":{"Treatment":"ctrl RNAi","Days of adulthood":"6"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva012_TAGII",
"Factors":{"Treatment":"ctrl RNAi","Days of adulthood":"12"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva013_TAGII",
"Factors":{"Treatment":"tif-IA RNAi","Days of adulthood":"2"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva014_TAGII",
"Factors":{"Treatment":"tif-IA RNAi","Days of adulthood":"6"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva015_TAGII",
"Factors":{"Treatment":"tif-IA RNAi","Days of adulthood":"12"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva016_TAGII",
"Factors":{"Treatment":"ncl-1 RNAi","Days of adulthood":"2"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva017_TAGII",
"Factors":{"Treatment":"ncl-1 RNAi","Days of adulthood":"6"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva018_TAGII",
"Factors":{"Treatment":"ncl-1 RNAi","Days of adulthood":"12"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva019_TAGII",
"Factors":{"Treatment":"ctrl RNAi","Days of adulthood":"2"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva020_TAGII",
"Factors":{"Treatment":"ctrl RNAi","Days of adulthood":"6"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva021_TAGII",
"Factors":{"Treatment":"ctrl RNAi","Days of adulthood":"12"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva022_TAGII",
"Factors":{"Treatment":"tif-IA RNAi","Days of adulthood":"2"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva023_TAGII",
"Factors":{"Treatment":"tif-IA RNAi","Days of adulthood":"6"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva024_TAGII",
"Factors":{"Treatment":"tif-IA RNAi","Days of adulthood":"12"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva025_TAGII",
"Factors":{"Treatment":"ncl-1 RNAi","Days of adulthood":"2"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva026_TAGII",
"Factors":{"Treatment":"ncl-1 RNAi","Days of adulthood":"6"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva027_TAGII",
"Factors":{"Treatment":"ncl-1 RNAi","Days of adulthood":"12"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva028_TAGII",
"Factors":{"Treatment":"ctrl RNAi","Days of adulthood":"2"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva029_TAGII",
"Factors":{"Treatment":"ctrl RNAi","Days of adulthood":"6"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva030_TAGII",
"Factors":{"Treatment":"ctrl RNAi","Days of adulthood":"12"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva031_TAGII",
"Factors":{"Treatment":"tif-IA RNAi","Days of adulthood":"2"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva032_TAGII",
"Factors":{"Treatment":"tif-IA RNAi","Days of adulthood":"6"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva033_TAGII",
"Factors":{"Treatment":"tif-IA RNAi","Days of adulthood":"12"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva034_TAGII",
"Factors":{"Treatment":"ncl-1 RNAi","Days of adulthood":"2"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva035_TAGII",
"Factors":{"Treatment":"ncl-1 RNAi","Days of adulthood":"6"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva036_TAGII",
"Factors":{"Treatment":"ncl-1 RNAi","Days of adulthood":"12"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva037_TAGII",
"Factors":{"Treatment":"ctrl RNAi","Days of adulthood":"2"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva038_TAGII",
"Factors":{"Treatment":"ctrl RNAi","Days of adulthood":"6"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva039_TAGII",
"Factors":{"Treatment":"ctrl RNAi","Days of adulthood":"12"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva040_TAGII",
"Factors":{"Treatment":"tif-IA RNAi","Days of adulthood":"2"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva041_TAGII",
"Factors":{"Treatment":"tif-IA RNAi","Days of adulthood":"6"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva042_TAGII",
"Factors":{"Treatment":"tif-IA RNAi","Days of adulthood":"12"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva043_TAGII",
"Factors":{"Treatment":"ncl-1 RNAi","Days of adulthood":"2"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva044_TAGII",
"Factors":{"Treatment":"ncl-1 RNAi","Days of adulthood":"6"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
},
{
"Subject ID":"-",
"Sample ID":"191111_FW_1_project_Ermolaeva045_TAGII",
"Factors":{"Treatment":"ncl-1 RNAi","Days of adulthood":"12"},
"Additional sample data":{"RAW_FILE_NAME":"191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Worms were washed and pelleted, M9 media was removed with ~100µl remaining in the sample, and the sample was snap frozen.","SAMPLE_TYPE":"Worms"},

"TREATMENT":{"TREATMENT_SUMMARY":"Ncl-1 and tif-IA knockdown was achieved by feeding the worms for 2 generations with bacteria (from the Ahringer RNAi feeding library, Source BioScience Ltd.) producing the corresponding dsRNA. Worms were cultured under standard conditions as described in ‘Wormbook’, F1 was grown at 15°C and F2 at 20°C, and F2 animals were grown on RNAi until days 2, 6 or 12 of adulthood."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Triacylglycerols were extracted from C. elegans by successive addition of PBS pH 7.4, methanol, chloroform, and saline to a final ratio of 14:34:35:17. Evaporation of the organic layer yielded a lipid film that was dissolved in methanol and subjected to UPLC-MS/MS.","EXTRACT_STORAGE":"-20℃"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"Chromatographic separation of triacylglycerols was carried out on an Acquity BEH C8 column (1.7 μm, 2.1×100 mm, Waters, Milford, MA) using an Acquity UHPLC.","CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Waters Acquity H-Class","COLUMN_NAME":"Waters Acquity BEH C8 (100 x 2.1mm, 1.7um)"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"ABI Sciex 5500 QTrap","INSTRUMENT_TYPE":"QTRAP","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"Targeted MRM with pre-optimized settings and subsequent automated integration of selected signals using Analyst 1.6.2 or Analyst 1.6.3 (Sciex)."},

"MS_METABOLITE_DATA":{
"Units":"relative units",

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