{ "METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000943","ANALYSIS_ID":"AN001547","VERSION":"1","CREATED_ON":"March 7, 2018, 5:48 pm"}, "PROJECT":{"PROJECT_TITLE":"FLUTE: Functionalizing Lists of Unknown TB Entities","PROJECT_TYPE":"NIH U19","PROJECT_SUMMARY":"Functional studies of proteins of unknown function in Mycobacterium tuberculosis","INSTITUTE":"Texas A&M University","LAST_NAME":"Ioerger","FIRST_NAME":"Thomas","ADDRESS":"310 Bright Bldg, TAMUS 3112, College Station, TX 77843","EMAIL":"ioerger@cs.tamu.edu","PHONE":"9792291494","FUNDING_SOURCE":"NIH U19 AI107774"}, "STUDY":{"STUDY_TITLE":"Activity-Based Metabolic Profiling of M. tuberculosis H37Rv Rv3916c","STUDY_TYPE":"timecourse","STUDY_SUMMARY":"These experiments involve incubating various proteins of unknown function in the M. tuberculosis genome with whole-cell lysate and assaying changes in metabolite levels over time (e.g. 0, 15, 30min) to try to infer what reaction they might catalyze.","INSTITUTE":"Weill Cornell Medical College, NY","LAST_NAME":"Rhee","FIRST_NAME":"Kyu","ADDRESS":"Department of Microbiology and Immunology, Weill Cornell Medical College, 1300 York Avenue, New York, NY","EMAIL":"kyr9001@med.cornell.edu","PHONE":"979-229-1494"}, "SUBJECT":{"SUBJECT_TYPE":"Bacteria","SUBJECT_SPECIES":"Mycobacterium tuberculosis H37Rv","TAXONOMY_ID":"83332"}, "SUBJECT_SAMPLE_FACTORS":[ { "Subject ID":"Rv3916c", "Sample ID":"3916_A_0", "Factors":{"Time":"-","Protein":"Active"}, "Additional sample data":{"Replicate":"A"} }, { "Subject ID":"Rv3916c", "Sample ID":"3916_B_0", "Factors":{"Time":"-","Protein":"Active"}, "Additional sample data":{"Replicate":"B"} }, { "Subject ID":"Rv3916c", "Sample ID":"3916_C_0", "Factors":{"Time":"-","Protein":"Active"}, "Additional sample data":{"Replicate":"C"} }, { "Subject ID":"Rv3916c", "Sample ID":"3916_A_15", "Factors":{"Time":"15","Protein":"Active"}, "Additional sample data":{"Replicate":"A"} }, { "Subject ID":"Rv3916c", "Sample ID":"3916_B_15", "Factors":{"Time":"15","Protein":"Active"}, "Additional sample data":{"Replicate":"B"} }, { "Subject ID":"Rv3916c", "Sample ID":"3916_C_15", "Factors":{"Time":"15","Protein":"Active"}, "Additional sample data":{"Replicate":"C"} }, { "Subject ID":"Rv3916c", "Sample ID":"3916_A_30", "Factors":{"Time":"30","Protein":"Active"}, "Additional sample data":{"Replicate":"A"} }, { "Subject ID":"Rv3916c", "Sample ID":"3916_B_30", "Factors":{"Time":"30","Protein":"Active"}, "Additional sample data":{"Replicate":"B"} }, { "Subject ID":"Rv3916c", "Sample ID":"3916_C_30", "Factors":{"Time":"30","Protein":"Active"}, "Additional sample data":{"Replicate":"C"} }, { "Subject ID":"Rv3916c", "Sample ID":"Inactivated-3916_A_0", "Factors":{"Time":"-","Protein":"Inactivated"}, "Additional sample data":{"Replicate":"A"} }, { "Subject ID":"Rv3916c", "Sample ID":"Inactivated-3916_B_0", "Factors":{"Time":"-","Protein":"Inactivated"}, "Additional sample data":{"Replicate":"B"} }, { "Subject ID":"Rv3916c", "Sample ID":"Inactivated-3916_C_0", "Factors":{"Time":"-","Protein":"Inactivated"}, "Additional sample data":{"Replicate":"C"} }, { "Subject ID":"Rv3916c", "Sample ID":"Inactivated-3916_A_15", "Factors":{"Time":"15","Protein":"Inactivated"}, "Additional sample data":{"Replicate":"A"} }, { "Subject ID":"Rv3916c", "Sample ID":"Inactivated-3916_B_15", "Factors":{"Time":"15","Protein":"Inactivated"}, "Additional sample data":{"Replicate":"B"} }, { "Subject ID":"Rv3916c", "Sample ID":"Inactivated-3916_C_15", "Factors":{"Time":"15","Protein":"Inactivated"}, "Additional sample data":{"Replicate":"C"} }, { "Subject ID":"Rv3916c", "Sample ID":"Inactivated-3916_A_30", "Factors":{"Time":"30","Protein":"Inactivated"}, "Additional sample data":{"Replicate":"A"} }, { "Subject ID":"Rv3916c", "Sample ID":"Inactivated-3916_B_30", "Factors":{"Time":"30","Protein":"Inactivated"}, "Additional sample data":{"Replicate":"B"} }, { "Subject ID":"Rv3916c", "Sample ID":"Inactivated-3916_C_30", "Factors":{"Time":"30","Protein":"Inactivated"}, "Additional sample data":{"Replicate":"C"} } ], "COLLECTION":{"COLLECTION_SUMMARY":"Rv3916c from M. tuberculosis H37Rv was expressed in E. coli cells and purified.","SAMPLE_TYPE":"Cultured cells"}, "TREATMENT":{"TREATMENT_SUMMARY":"Protein was incubated with whole-cell lysate in either Active form in Inactive (heat-inactivated)."}, "SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Protein was incubated with whole-cell lysate in either Active form or Inactive (heat-inactivated)."}, "CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"Aqueous normal phase diamond hydride","CHROMATOGRAPHY_TYPE":"Normal phase","INSTRUMENT_NAME":"Agilent 1290","COLUMN_NAME":"Microsolv Diamond hydride (150mm, 4um)"}, "ANALYSIS":{"ANALYSIS_TYPE":"MS"}, "MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"Agilent 6220 TOF","INSTRUMENT_TYPE":"TOF","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_RESULTS_FILE":"ST000943_AN001547_Results.txt UNITS:peak counts"} }