Summary of Study ST003818
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002389. The data can be accessed directly via it's Project DOI: 10.21228/M8TR66 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003818 |
| Study Title | Irisin Attenuates Postmenopausal Osteoporosis by Upregulating Phosphatidylcholine Metabolism via the Wnt/β-catenin Pathway |
| Study Summary | Irisin can ameliorate postmenopausal osteoporosis (PMOP) in ovariectomized (OVX) mice by promoting osteogenesis, inhibiting adipogenesis, and regulating the bone-fat balance. Similarly, irisin enhances the osteogenic differentiation of human bone marrow mesenchymal stem cells (BMSCs) in vitro while reducing their adipogenic differentiation. Metabolomic analysis of mouse plasma revealed that the therapeutic effects of irisin are associated with pathways such as glycerophospholipid metabolism, wherein the metabolite phosphatidylcholine (PC) plays a significant role. Irisin can upregulate the metabolism of PC in OVX mice, thereby improving OP. We further investigated the metabolic role of PC and its mechanisms. By administering PC to BMSCs, we observed its effects on BMSCs and the underlying mechanisms. It was found that PC activates the canonical Wnt/β-catenin signaling pathway, promoting the osteogenic differentiation of BMSCs while inhibiting their adipogenic differentiation. |
| Institute | Huizhou Central People's Hospital |
| Department | Department of Traumatology and Orthopaedic Surgery, Orthopaedic Institute |
| Last Name | Zhang |
| First Name | Zhiwen |
| Address | No. 41, Eling North Road, Huicheng District, Huizhou City, Guangdong Province, Guangdong Province, Huizhou City, Guangdong Province, 516001, China |
| 13794539802@163.com | |
| Phone | 13794539802 |
| Submit Date | 2025-03-20 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2025-04-21 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR002389 |
| Project DOI: | doi: 10.21228/M8TR66 |
| Project Title: | Irisin Attenuates Postmenopausal Osteoporosis by Upregulating Phosphatidylcholine Metabolism via the Wnt/β-catenin Pathway |
| Project Summary: | Irisin can ameliorate postmenopausal osteoporosis (PMOP) in ovariectomized (OVX) mice by promoting osteogenesis, inhibiting adipogenesis, and regulating the bone-fat balance. Similarly, irisin enhances the osteogenic differentiation of human bone marrow mesenchymal stem cells (BMSCs) in vitro while reducing their adipogenic differentiation. Metabolomic analysis of mouse plasma revealed that the therapeutic effects of irisin are associated with pathways such as glycerophospholipid metabolism, wherein the metabolite phosphatidylcholine (PC) plays a significant role. Irisin can upregulate the metabolism of PC in OVX mice, thereby improving OP. We further investigated the metabolic role of PC and its mechanisms. By administering PC to BMSCs, we observed its effects on BMSCs and the underlying mechanisms. It was found that PC activates the canonical Wnt/β-catenin signaling pathway, promoting the osteogenic differentiation of BMSCs while inhibiting their adipogenic differentiation. |
| Institute: | Huizhou Central People's Hospital |
| Department: | Department of Traumatology and Orthopaedic Surgery, Orthopaedic Institute |
| Last Name: | Zhang |
| First Name: | Zhiwen |
| Address: | No. 41, Eling North Road, Huicheng District, Huizhou City, Guangdong Province, Guangdong Province, Huizhou City, Guangdong Province, 516001, China |
| Email: | 13794539802@163.com |
| Phone: | 13794539802 |
Subject:
| Subject ID: | SU003952 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Sample source | Treatment |
|---|---|---|---|
| SA418810 | OVX_2 | plasma | OVX + normal saline |
| SA418811 | OVX_6 | plasma | OVX + normal saline |
| SA418812 | OVX_5 | plasma | OVX + normal saline |
| SA418813 | OVX_4 | plasma | OVX + normal saline |
| SA418814 | OVX_3 | plasma | OVX + normal saline |
| SA418815 | OVX_1 | plasma | OVX + normal saline |
| SA418816 | r_irisin_2 | plasma | OVX + r-irisin |
| SA418817 | r_irisin_6 | plasma | OVX + r-irisin |
| SA418818 | r_irisin_5 | plasma | OVX + r-irisin |
| SA418819 | r_irisin_4 | plasma | OVX + r-irisin |
| SA418820 | r_irisin_3 | plasma | OVX + r-irisin |
| SA418821 | r_irisin_1 | plasma | OVX + r-irisin |
| SA418822 | Sham_1 | plasma | Sham + normal saline |
| SA418823 | Sham_2 | plasma | Sham + normal saline |
| SA418824 | Sham_3 | plasma | Sham + normal saline |
| SA418825 | Sham_4 | plasma | Sham + normal saline |
| SA418826 | Sham_5 | plasma | Sham + normal saline |
| SA418827 | Sham_6 | plasma | Sham + normal saline |
| SA418804 | P_QC1_240929092428 | QC | Quality Control |
| SA418805 | P_QC2_240929121903 | QC | Quality Control |
| SA418806 | P_QC3 | QC | Quality Control |
| SA418807 | N_QC1_240929152935 | QC | Quality Control |
| SA418808 | N_QC2_240929182422 | QC | Quality Control |
| SA418809 | N_QC3 | QC | Quality Control |
| Showing results 1 to 24 of 24 |
Collection:
| Collection ID: | CO003945 |
| Collection Summary: | Prepare appropriate blood collection utensils in advance, such as syringes, blood collection tubes (containing anticoagulants, commonly heparin sodium or EDTA-K2, etc.), centrifuges, etc., and ensure that the utensils are clean and sterile. Suitable for collecting a small amount of blood. Fix the mouse, soak or wipe the tail with warm water to dilate the blood vessels, and then cut a small opening at the tip of the tail with scissors or a blade to let the blood flow out and collect it with a blood collection tube. Multiple blood collections are possible, but the amount of blood collected each time should not be too much. |
| Sample Type: | Blood (plasma) |
Treatment:
| Treatment ID: | TR003961 |
| Treatment Summary: | In an environment with temperature control, 4 to 5 mice are housed in each cage, and the light/dark cycle is set at 12 hours. After a two-week adaptation period, 18 mice with a body weight ranging from 19.4 to 22.5 grams are selected and randomly divided into three groups: the OVX + r-irisin group, the OVX + normal saline group, and the Sham + normal saline group, with 6 mice in each group. Starting from the third day after the operation, a vaginal cell smear is taken from each animal once a day for 7 consecutive days. A successful bilateral ovariectomy is confirmed by screening the vaginal cell smear, which shows the absence of keratosis. OVX + r-irisin group: Ovariectomy is performed. One week after the operation, the mice start to receive intraperitoneal injections of 100 μg/kg irisin (MCE, HY-P70665, 100 μg) twice a week for 5 consecutive weeks. OVX + normal saline group: Ovariectomy is carried out. One week after the operation, the mice begin to receive intraperitoneal injections of an equal volume of normal saline twice a week for 5 consecutive weeks. Sham + normal saline group: A sham operation is performed. One week after the operation, the mice start to receive intraperitoneal injections of an equal volume of normal saline twice a week for 5 consecutive weeks. Five weeks after the treatment, the animals are euthanized. |
Sample Preparation:
| Sampleprep ID: | SP003958 |
| Sampleprep Summary: | Immediately invert the blood collection tube gently after collecting the blood to fully mix the blood with the anticoagulant and prevent blood clotting. Then, put the blood collection tube into a centrifuge and centrifuge it at a speed of 3,000 - 4,000 revolutions per minute for 10 - 15 minutes. After centrifugation, the blood will be stratified, and the light yellow transparent liquid in the upper layer is the plasma. Carefully transfer the plasma to a clean centrifuge tube or other container with a pipette or straw, avoiding sucking the blood cells in the lower layer and the buffy coat in the middle. |
Combined analysis:
| Analysis ID | AN006277 | AN006278 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Waters Acquity | Waters Acquity |
| Column | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | Triple TOF | Triple TOF |
| MS instrument name | ABI Sciex 5600 TripleTOF | ABI Sciex 5600 TripleTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Relative abundance | Relative abundance |
Chromatography:
| Chromatography ID: | CH004761 |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| Column Temperature: | 40℃ |
| Flow Gradient: | 0 - 5 min: A 95%→70%, B 5%→30%, flow rate 0.2 mL/min → 0.5 mL/min; 5 - 15 min: A 70%→20%, B 30%→80%, flow rate 0.5 mL/min; 15 - 17 min: A 20%→40%, B 80%→60%, flow rate 0.5 mL/min → 0.2 mL/min |
| Flow Rate: | 0.2-0.5 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS005979 |
| Analysis ID: | AN006277 |
| Instrument Name: | ABI Sciex 5600 TripleTOF |
| Instrument Type: | Triple TOF |
| MS Type: | ESI |
| MS Comments: | The raw data obtained from the LC-MS/MS analysis of fecal metabolites were first processed to remove background noise and correct any baseline shifts. Data files were converted into a compatible format mzML using ProteoWizard software. The resulting data were then analyzed using PeakView to identify peaks corresponding to metabolites based on their retention times and m/z values. Peak intensity values were normalized using internal standards to correct for potential variability in sample preparation and instrumental response. |
| Ion Mode: | POSITIVE |
| MS ID: | MS005980 |
| Analysis ID: | AN006278 |
| Instrument Name: | ABI Sciex 5600 TripleTOF |
| Instrument Type: | Triple TOF |
| MS Type: | ESI |
| MS Comments: | The raw data obtained from the LC-MS/MS analysis of fecal metabolites were first processed to remove background noise and correct any baseline shifts. Data files were converted into a compatible format mzML using ProteoWizard software. The resulting data were then analyzed using PeakView to identify peaks corresponding to metabolites based on their retention times and m/z values. Peak intensity values were normalized using internal standards to correct for potential variability in sample preparation and instrumental response. |
| Ion Mode: | NEGATIVE |
