Summary of Study ST003730

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002316. The data can be accessed directly via it's Project DOI: 10.21228/M88838 This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003730
Study TitleVirus infection of honey bee queens alters lipid profiles and indirectly suppresses a retinue pheromone component via reducing ovary mass
Study SummaryIn this study, we investigated the effects of viral infection on honey bee queens by conducting controlled laboratory infections and performing lipidomic profiling. Our analysis revealed that virus infections specifically reduce the queen retinue pheromone (QRP) component methyl oleate, a key signal in colony communication. Additionally, lipidomics data suggest that viral infection leads to a decrease in triacylglycerol abundances, potentially impacting queen energy metabolism and pheromone production.
Institute
University of British Columbia
DepartmentBiochemistry and Molecular Biology, Life Sciences Institute
LaboratoryFoster Lab
Last NameAlcazar Magana
First NameArmando
Address2350 Health Sciences Mall
Emailarmando.alcazarmagana@ubc.ca
Phone5416097172
Submit Date2025-02-13
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2025-03-05
Release Version1
Armando Alcazar Magana Armando Alcazar Magana
https://dx.doi.org/10.21228/M88838
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR002316
Project DOI:doi: 10.21228/M88838
Project Title:Virus infection of honey bee queens
Project Summary:In this study, we investigated the impact of infection on honey bee queens by conducting controlled laboratory infections. We then analyzed their lipid profiles using head extracts.
Institute:University of British Columbia
Department:Biochemistry and Molecular Biology, Life Sciences Institute
Last Name:Alcazar Magana
First Name:Armando
Address:2350 Health Sciences Mall, Vancouver, BC, V6T1Z3, Canada
Email:armando.alcazarmagana@ubc.ca
Phone:5416097172

Subject:

Subject ID:SU003862
Subject Type:Insect
Subject Species:Apis mellifera
Taxonomy ID:7460

Factors:

Subject type: Insect; Subject species: Apis mellifera (Factor headings shown in green)

mb_sample_id local_sample_id Treatment Bee type
SA407412CQ24control Cage Trial
SA407413CQ20control Cage Trial
SA407414CQ28control Cage Trial
SA407415CQ17control Cage Trial
SA407416CQ5control Cage Trial
SA407417CQ13control Cage Trial
SA407418CQ9control Cage Trial
SA407419CQ6control Cage Trial
SA407420CQ3control Cage Trial
SA407374FQ14Control Field Trial
SA407375FQ31Control Field Trial
SA407376FQ28Control Field Trial
SA407377FQ27Control Field Trial
SA407378FQ22Control Field Trial
SA407379FQ21Control Field Trial
SA407380FQ19Control Field Trial
SA407381FQ18Control Field Trial
SA407382FQ1Control Field Trial
SA407383FQ11Control Field Trial
SA407384FQ9Control Field Trial
SA407385FQ4Control Field Trial
SA407386FQ5Control Field Trial
SA407387FQ32Control Field Trial
SA407388FQ10Control Field Trial
SA407389FQ17Infected Field Trial
SA407390FQ2Infected Field Trial
SA407391FQ3Infected Field Trial
SA407392FQ30Infected Field Trial
SA407393FQ29Infected Field Trial
SA407394FQ16Infected Field Trial
SA407395FQ13Infected Field Trial
SA407396FQ24Infected Field Trial
SA407397FQ23Infected Field Trial
SA407398FQ6Infected Field Trial
SA407399FQ7Infected Field Trial
SA407400FQ20Infected Field Trial
SA407401FQ8Infected Field Trial
SA407402FQ12Infected Field Trial
SA407421CQ15live Cage Trial
SA407422CQ14live Cage Trial
SA407423CQ11live Cage Trial
SA407424CQ22live Cage Trial
SA407425CQ23live Cage Trial
SA407426CQ25live Cage Trial
SA407427CQ4live Cage Trial
SA407428CQ1live Cage Trial
SA407429CQ8live Cage Trial
SA407403CQ18UV Cage Trial
SA407404CQ12UV Cage Trial
SA407405CQ19UV Cage Trial
SA407406CQ10UV Cage Trial
SA407407CQ29UV Cage Trial
SA407408CQ7UV Cage Trial
SA407409CQ27UV Cage Trial
SA407410CQ2UV Cage Trial
SA407411CQ16UV Cage Trial
Showing results 1 to 56 of 56

Collection:

Collection ID:CO003855
Collection Summary:The samples were derived entirely from cage trials and field according to standard queen rearing methods as described by Chapman A, et al., Scientific Reports. 2024;14(1):17285.
Sample Type:queen bee head

Treatment:

Treatment ID:TR003871
Treatment Summary:The samples were derived entirely from cage trials and field samples as described by Chapman A, et al., Scientific Reports. 2024;14(1):17285. Briefly, in the cage trial, young (< 1 month old) queens were microinjected with either saline (N=9, control group), a live virus inoculum (N-9, live group), or a UV-inactivated virus inoculum (N=9, UV group). The queens were observed for seven days after injection, then were euthanized, dissected, and samples were stored at -70 °C. For the field samples, the queens were blindly divided into two groups, with one receiving a saline injection (N=15, control) and the other receiving the same live virus inoculum described above (N=14, Infected)

Sample Preparation:

Sampleprep ID:SP003868
Sampleprep Summary:Two-phase extraction was conducted according to methods previously described at J. Lipid Res. 49, 1137–1146 (2008)
Processing Storage Conditions:-80℃
Extract Storage:4℃

Combined analysis:

Analysis ID AN006120 AN006121
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Vanquish Thermo Vanquish
Column Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Bruker Impact II Bruker Impact II
Ion Mode POSITIVE NEGATIVE
Units Relative Abundance Relative Abundance

Chromatography:

Chromatography ID:CH004648
Chromatography Summary:ESI+. Lipids were separated on an ACQUITY UPLC CSH C18 analytical column (130Å, 1.7 µm, 2.1 mm X 100 mm, Waters) with a multi-step elution gradient optimized to resolve polar lipids such as hydroxylated fatty acids analyzed.
Instrument Name:Thermo Vanquish
Column Name:Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um)
Column Temperature:65 °C
Flow Gradient:0 min (20% B), 0–2 min (20% B), 2–11 min (80% B), 11–11.5 min (99% B), 11.5–13.2 min (99% B), 13.2–14 min (5% B), and 14–18 min (5% B).
Flow Rate:0.4 ml/min
Solvent A:100% water; 10 mM ammonium formate; 0.1% formic acid
Solvent B:10% acetonitrile/90% isopropanol; 10 mM ammonium formate; 0.1% formic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS005826
Analysis ID:AN006120
Instrument Name:Bruker Impact II
Instrument Type:QTOF
MS Type:ESI
MS Comments:For positive ion mode, the mass spectrometer settings were as follows: capillary voltage of 4500 V, nebulizer gas pressure of 2.0 bar, dry gas flow rate of 9 L min-1, dry gas temperature of 220°C, mass scan range of 60-1300 m/z, and a total cycle time of 0.6 s. To obtain comprehensive structural information, collision energy of 20 V was ramped through each MS/MS scan from 100 to 250%.
Ion Mode:POSITIVE
  
MS ID:MS005827
Analysis ID:AN006121
Instrument Name:Bruker Impact II
Instrument Type:QTOF
MS Type:ESI
MS Comments:For negative ionization mode, the capillary voltage was set at -3500 V.
Ion Mode:NEGATIVE
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