Summary of Study ST003327

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002069. The data can be accessed directly via it's Project DOI: 10.21228/M85R82 This work is supported by NIH grant, U2C- DK119886.

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Study IDST003327
Study TitleControl Of Major Bleeding After Trauma (COMBAT) Clinical Research Trial Metabolomics
Study SummaryDenver Health has conducted the Control of Major Bleeding After Trauma (COMBAT) clinical research study, which aimed to improve trauma care for all patients (https://www.denverhealth.org/services/trauma/for-professionals-2/research-publications/combattrial). The study began in April 2014 and concluded in March of 2017. In collaboration with the University of Colorado Anschutz Medical Campus, longitudinal plasma samples collected from the COMBAT trial were analyzed by MS-based metabolomics and proteomics (data available on ProteomeXchange).
Institute
University of Colorado Anschutz Medical Campus
DepartmentBiochemistry and Molecular Genetics
LaboratoryThe Hansen and D'Alessandro Labs
Last NameLaCroix
First NameIan
AddressRC1-S L18-9403G E 17th Ave. Aurora, CO 80045
Emailian.lacroix@cuanschutz.edu
Phone(303) 724-0096
Submit Date2024-07-01
Total Subjects118
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2024-08-08
Release Version1
Ian LaCroix Ian LaCroix
https://dx.doi.org/10.21228/M85R82
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR002069
Project DOI:doi: 10.21228/M85R82
Project Title:Control Of Major Bleeding After Trauma (COMBAT) Clinical Research Study Metabolomics
Project Summary:Denver Health has conducted the Control of Major Bleeding After Trauma (COMBAT) clinical research study, which aimed to improve trauma care for all patients (https://www.denverhealth.org/services/trauma/for-professionals-2/research-publications/combattrial). The study began in April 2014 and concluded in March of 2017. In collaboration with the University of Colorado Anschutz Medical Campus, longitudinal plasma samples collected from the COMBAT trial were analyzed by MS-based metabolomics and proteomics (data available on ProteomeXchange).
Institute:University of Colorado Anschutz Medical Campus
Department:Biochemistry and Molecular Genetics
Laboratory:The Hansen and D'Alessandro Labs
Last Name:LaCroix
First Name:Ian
Address:RC1-S L18-9403G E 17th Ave. Aurora, CO 80045
Email:ian.lacroix@cuanschutz.edu
Phone:(303) 724-0096
Publications:doi:10.1001/jamasurg.2019.5085, DOI: 10.1016/S0140-6736(18)31553-8, DOI: 10.1097/SHK.0000000000000376

Subject:

Subject ID:SU003448
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Mammals

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Time_Point Randomization
SA361635C-086_01ED Control
SA361636C-070_01ED Control
SA361637C-071_01ED Control
SA361638C-076_01ED Control
SA361639C-078_01ED Control
SA361640C-079_01ED Control
SA361641C-084_01ED Control
SA361642C-013_01ED Control
SA361643C-065_01ED Control
SA361644C-012_01ED Control
SA361645C-090_01ED Control
SA361646C-091_01ED Control
SA361647C-099_01ED Control
SA361648C-102_01ED Control
SA361649C-105_01ED Control
SA361650C-066_01ED Control
SA361651C-019_01ED Control
SA361652C-113_01ED Control
SA361653C-023_01ED Control
SA361654C-032_01ED Control
SA361655C-034_01ED Control
SA361656C-035_01ED Control
SA361657C-036_01ED Control
SA361658C-037_01ED Control
SA361659C-038_01ED Control
SA361660C-043_01ED Control
SA361661C-060_01ED Control
SA361662C-045_01ED Control
SA361663C-049_01ED Control
SA361664C-051_01ED Control
SA361665C-055_01ED Control
SA361666C-020_01ED Control
SA361667C-057_01ED Control
SA361668C-009_01ED Control
SA361669C-015_01ED Control
SA361670C-004_01ED Control
SA361671C-130_01ED Control
SA361672C-137_01ED Control
SA361673C-128_01ED Control
SA361674C-138_01ED Control
SA361675C-006_01ED Control
SA361676C-134_01ED Control
SA361677C-140_01ED Control
SA361678C-005_01ED Control
SA361679C-132_01ED Control
SA361680C-142_01ED Control
SA361681C-114_01ED Experimental
SA361682C-074_01ED Experimental
SA361683C-136_01ED Experimental
SA361684C-061_01ED Experimental
SA361685C-063_01ED Experimental
SA361686C-077_01ED Experimental
SA361687C-018_01ED Experimental
SA361688C-002_01ED Experimental
SA361689C-073_01ED Experimental
SA361690C-064_01ED Experimental
SA361691C-059_01ED Experimental
SA361692C-133_01ED Experimental
SA361693C-067_01ED Experimental
SA361694C-008_01ED Experimental
SA361695C-056_01ED Experimental
SA361696C-058_01ED Experimental
SA361697C-041_01ED Experimental
SA361698C-030_01ED Experimental
SA361699C-031_01ED Experimental
SA361700C-144_01ED Experimental
SA361701C-033_01ED Experimental
SA361702C-143_01ED Experimental
SA361703C-026_01ED Experimental
SA361704C-141_01ED Experimental
SA361705C-042_01ED Experimental
SA361706C-080_01ED Experimental
SA361707C-044_01ED Experimental
SA361708C-046_01ED Experimental
SA361709C-022_01ED Experimental
SA361710C-047_01ED Experimental
SA361711C-048_01ED Experimental
SA361712C-021_01ED Experimental
SA361713C-052_01ED Experimental
SA361714C-054_01ED Experimental
SA361715C-016_01ED Experimental
SA361716C-029_01ED Experimental
SA361717C-094_01ED Experimental
SA361718C-083_01ED Experimental
SA361719C-093_01ED Experimental
SA361720C-011_01ED Experimental
SA361721C-098_01ED Experimental
SA361722C-126_01ED Experimental
SA361723C-124_01ED Experimental
SA361724C-089_01ED Experimental
SA361725C-104_01ED Experimental
SA361726C-121_01ED Experimental
SA361727C-085_01ED Experimental
SA361728C-109_01ED Experimental
SA361729C-106_01ED Experimental
SA361730C-081_01ED Experimental
SA361731C-014_01ED Experimental
SA361732C-120_01ED Experimental
SA361733C-110_01ED Experimental
SA361734C-111_01ED Experimental
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Collection:

Collection ID:CO003441
Collection Summary:The field blood sample was drawn immediately on the scene of injury before the administration of any therapy. Immediately on hospital arrival, collection of the next set of blood samples occurs, followed by samples at 2, 4, 6, 12, 24, 48, and 72 h and days 5 and 7 after injury. (DOI: 10.1097/SHK.0000000000000376)
Sample Type:Blood (plasma)
Storage Conditions:-80℃
Collection Vials:anticoagulated tubes containing sodium citrate and lithium heparin

Treatment:

Treatment ID:TR003457
Treatment Summary:The main goal of the COMBAT trial was to evaluate the effectiveness of initiating resuscitation with plasma compared to the standard practice of using normal saline. Patients were randomly assigned to either treatment during their ambulance ride by opening a preloaded, sealed cooler. This cooler contained either two units of frozen plasma (FP24, approximately 250 mL each), which were immediately thawed and administered, or a dummy load, which instructed paramedics to provide normal saline as per the current standard of care. (As written in DOI: 10.1097/SHK.0000000000000376)

Sample Preparation:

Sampleprep ID:SP003455
Sampleprep Summary:Frozen plasma aliquots (10 μL) were extracted 1:25 in ice cold extraction solution (methanol:acetonitrile:water 5:3:2 v/v/v). Samples were vortexed for 30 min at 4℃, prior to centrifugation for 10 min at 15,000g at 4℃.
Sampleprep Protocol Comments:as described in DOI: 10.3389/fphys.2020.593841 , DOI: 10.3324/haematol.2019.229930

Combined analysis:

Analysis ID AN005450 AN005451
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Vanquish Thermo Vanquish
Column Phenomenex Kinetex C18 (150 x 2.1mm,1.7um) Phenomenex Kinetex C18 (150 x 2.1mm,1.7um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode NEGATIVE POSITIVE
Units area under curve area under curve

Chromatography:

Chromatography ID:CH004137
Chromatography Summary:Ion Mode: NEGATIVE
Instrument Name:Thermo Vanquish
Column Name:Phenomenex Kinetex C18 (150 x 2.1mm,1.7um)
Column Temperature:45
Flow Gradient:Details are available upon reasonable request.
Flow Rate:0.3mL/min
Solvent A:Details are available upon reasonable request.
Solvent B:Details are available upon reasonable request.
Chromatography Type:Reversed phase
  
Chromatography ID:CH004138
Chromatography Summary:Ion Mode: Positive
Instrument Name:Thermo Vanquish
Column Name:Phenomenex Kinetex C18 (150 x 2.1mm,1.7um)
Column Temperature:45
Flow Gradient:Details are available upon reasonable request.
Flow Rate:0.3mL/min
Solvent A:Details are available upon reasonable request.
Solvent B:Details are available upon reasonable request.
Chromatography Type:Reversed phase

MS:

MS ID:MS005176
Analysis ID:AN005450
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Analyses were performed using a Vanquish UHPLC coupled online to a Q Exactive mass spectrometer (ThermoFisher). Samples were analyzed using a 1 min and 5 minute gradient-based method, spectra were searched in Maven, and data are presented as units of relative intensity.
Ion Mode:NEGATIVE
  
MS ID:MS005177
Analysis ID:AN005451
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Analyses were performed using a Vanquish UHPLC coupled online to a Q Exactive mass spectrometer (ThermoFisher). Samples were analyzed using a 1 min and 5 minute gradient-based method, spectra were searched in Maven, and data are presented as units of relative intensity.
Ion Mode:POSITIVE
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