Summary of Study ST002370

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001523. The data can be accessed directly via it's Project DOI: 10.21228/M8TQ5G This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002370
Study TitleThe impact of acute Colony Stimulating Factor 1 treatment on serum and liver metabolites in fed and fasted mice – SERUM
Study TypeDrug treatment
Study SummaryThe aim of the study was to investigate the impact of expanding tissue macrophage populations on systemic metabolism. Groups of male mice were treated with 4 x daily injections of Colony Stimulating Factor (1), followed by normal feeding or a 24 h fast from day 6 to day 7. Serum and liver were collected for GC-MS metabolomic analysis on a Shimadzu TQ8050.
Institute
Mater Research-The University of Queensland
Last NameIrvine
First NameKatharine
Address37 Kent St, Brisbane, Queensland, 4102, Australia
Emailkatharine.irvine@uq.edu.au
Phone+61734437655
Submit Date2022-11-28
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailGC-MS
Release Date2023-08-01
Release Version1
Katharine Irvine Katharine Irvine
https://dx.doi.org/10.21228/M8TQ5G
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001523
Project DOI:doi: 10.21228/M8TQ5G
Project Title:The impact of acute Colony Stimulating Factor 1 treatment on serum and liver metabolites in fed and fasted mice
Project Type:Drug treatment
Project Summary:Groups of male mice were treated with 4 x daily injections of Colony Stimulating Factor (1), followed by normal feeding or a 24 h fast from day 6 to day 7. Serum and liver were collected for GC-MS metabolomic analysis on a Shimadzu TQ8050NX.
Institute:University of Queensland
Department:Mater Research-UQ
Laboratory:Irvine
Last Name:Irvine
First Name:Katharine
Address:Translational Research Institute, 37 Kent St, Woolloongabba, 4102, QLD, Australia
Email:katharine.irvine@uq.edu.au
Phone:+61734437655

Subject:

Subject ID:SU002459
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammals

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA237298319Fasted/CSF1-Fc
SA237299322Fasted/CSF1-Fc
SA237300318Fasted/CSF1-Fc
SA237301321Fasted/CSF1-Fc
SA237302314Fasted/CSF1-Fc
SA237303316Fasted/Saline
SA237304311Fasted/Saline
SA237305320Fasted/Saline
SA237306312Fasted/Saline
SA237307serumPBQC_2Pooled Biological Quality Control
SA237308serumPBQC_1Pooled Biological Quality Control
SA237309serumPBQC_3Pooled Biological Quality Control
SA237310serumPBQC_6Pooled Biological Quality Control
SA237311serumPBQC_5Pooled Biological Quality Control
SA237312335Unfasted/CSF1-Fc
SA237313327Unfasted/CSF1-Fc
SA237314326Unfasted/CSF1-Fc
SA237315334Unfasted/CSF1-Fc
SA237316330Unfasted/CSF1-Fc
SA237317324Unfasted/Saline
SA237318333Unfasted/Saline
SA237319328Unfasted/Saline
SA237320332Unfasted/Saline
Showing results 1 to 23 of 23

Collection:

Collection ID:CO002452
Collection Summary:Groups of male mice were treated with 4 x daily injections of Colony Stimulating Factor (1), followed by normal feeding or a 24 h fast from day 6 to day 7. Serum was obtained from blood obtained from cardiac puncture under isofluorane anaesthesia.
Sample Type:serum

Treatment:

Treatment ID:TR002471
Treatment Summary:Groups of male mice were treated with 4 x daily injections of Colony Stimulating Factor (1), followed by normal feeding or a 24 h fast from day 6 to day 7.

Sample Preparation:

Sampleprep ID:SP002465
Sampleprep Summary:Metabolites were extracted from 50 µl serum using 150 µl methanol and 50 µl chloroform, including 13C-sorbitol and 13C,15N-valine internal standards, and 30 µl of clarified sera was prepared for GC-MS analysis. Liver metabolites were extracted from 20-40 mg liver tissue (sampled from the same region of the left lateral lobe) in 600 µl methanol:MilliQ water (3:1), including 13C-sorbitol and 13C,15N-valine internal standards. The tissue was cryomilled at 4°C. 110 µl chloroform was added to 440 µg homogenate which was transferred to a new tube and the sample thermomixed for 20 minutes then centrifuged at 13,000 rpm for 15 minutes. 30 µl supernatant was dried in inserts for GC-MS analysis. Dried samples for targeted analysis were derivatised online using the Shimadzu AOC6000 autosampler robot. Derivatisation was achieved by the addition of 25 µL methoxyamine hydrochloride (30 mg/mL in pyridine, Merck) followed by shaking at 37°C for 2h. Samples were then derivatised with 25 µL of N,O-bis (trimethylsilyl)trifluoroacetamide with trimethylchlorosilane (BSTFA with 1% TMCS, Thermo Scientific) for 1h at 37°C. The sample was allowed to equilibrate at room temperature for 1 h before 1 µL was injected onto the GC column using a hot needle technique. Split (1:10) injections were performed for each sample.

Chromatography:

Chromatography ID:CH002863
Instrument Name:Shimadzu TQ8050NX
Column Name:Agilent DB5-MS (30m)
Chromatography Type:None (Direct infusion)

Analysis:

Analysis ID:AN003865
Analysis Type:MS
Chromatography ID:CH002863
Num Factors:5
Num Metabolites:192
Units:area
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