Summary of Study ST002166

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001378. The data can be accessed directly via it's Project DOI: 10.21228/M8M12K This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002166
Study TitleUntargeted metabolomics profiling of mice urine after 5 Gy and 7.5 Gy total body radiation exposure at 24 hours - NMR Study.
Study TypeUntargeted metabolic profiling
Study SummaryComprehensive metabolomics profiling of mice urine after 5 Gy and 7.5 Gy total body radiation exposure at 24 hours
Institute of Nuclear Medicine and Allied Sciences
Last NameRana
First NamePoonam
AddressDRDO, Lucknow Road, Timarpur, Delhi, Delhi, 110054, India
Submit Date2021-11-29
Num Groups3
Total Subjects21
Num Males21
Analysis Type DetailNMR
Release Date2022-05-31
Release Version1
Poonam Rana Poonam Rana application/zip

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Project ID:PR001378
Project DOI:doi: 10.21228/M8M12K
Project Title:Untargeted metabolomics following radiation exposure
Project Type:Untargeted metabolic profiling
Project Summary:Comprehensive metabolomics profiling of mice urine after 5 Gy and 7.5 Gy total body radiation exposure at 24 hours using LC-MS and NMR.
Institute:Institute of Nuclear Medicine and Allied Sciences
Department:Metabolomics facility
Laboratory:Metabolomics facility
Last Name:Rana
First Name:Poonam
Address:DRDO, Lucknow Road, Timarpur, Delhi, Delhi, 110054, India
Funding Source:INM 313, UGC-JRF


Subject ID:SU002252
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090


Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA207601R_5 Gy 35Gy Irradiated
SA207602R_5 Gy 25Gy Irradiated
SA207603R_5 Gy 55Gy Irradiated
SA207604R_5 Gy 65Gy Irradiated
SA207605R_5 Gy 75Gy Irradiated
SA207606R_5 Gy 15Gy Irradiated
SA207607R_5 Gy 45Gy Irradiated
SA207608R_7.5 Gy 37.5Gy Irradiated
SA207609R_7.5 Gy 47.5Gy Irradiated
SA207610R_7.5 Gy 67.5Gy Irradiated
SA207611R_7.5 Gy 77.5Gy Irradiated
SA207612R_7.5 Gy 27.5Gy Irradiated
SA207613R_7.5 Gy 17.5Gy Irradiated
SA207614R_Ctrl 6Control
SA207615R_Ctrl 2Control
SA207616R_Ctrl 1Control
SA207617R_Ctrl 5Control
SA207618R_Ctrl 3Control
SA207619R_Ctrl 4Control
SA207620R_Ctrl 7Control
Showing results 1 to 20 of 20


Collection ID:CO002245
Collection Summary:Animals were acclimatized for 48 hrs in polypropylene cages. Urine samples were collected at 24 hrs post radiation exposure.
Sample Type:Urine
Storage Conditions:-80℃
Collection Tube Temp:4℃


Treatment ID:TR002264
Treatment Summary:Random categorization was done into three groups: control, 5 Gy (sub-lethal) and 7.5 Gy (lethal) irradiated (seven animals in each group). Mice were kept for 12 hrs light and dark cycle with room temperature (19–23°C) and humidity (45–65%) conditions properly maintained. Mice were exposed to total-body gamma radiation (field size; 35x35 cm) of 5 Gy and 7.5 Gy through Tele 60Co unit γ- irradiation facility (Bhabhatron II, Panacea Medical, Bangalore, India) with dose rate of 1 Gy/min. Control mice were sham irradiated.
Treatment:Radiation exposure
Treatment Dose:5 Gy and 7.5 Gy Gamma-60Cobalt irradiation

Sample Preparation:

Sampleprep ID:SP002258
Sampleprep Summary:150 µl of urine was mixed with 375 µl of deuterated phosphate buffer solution (pH 7.2- 7.4) containing 0.5 mM TSP prepared in D2O. The solution was then transferred to 5 mm NMR tube for data acquisition


Analysis ID:AN003549
Analysis Type:NMR
Results File:ST002166_AN003549_Results.txt


NMR ID:NM000239
Analysis ID:AN003549
Instrument Name:Bruker
Instrument Type:FT-NMR
NMR Experiment Type:1D-1H
Spectrometer Frequency:600MHz