Summary of Study ST002158

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001371. The data can be accessed directly via it's Project DOI: 10.21228/M8H691 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002158
Study TitleUntargeted serum metabolomic profiling for early detection of Schistosoma mekongi infection in mouse model
Study SummaryMekong schistosomiasis is a parasitic disease caused by blood flukes in the Lao People’s Democratic Republic and in Cambodia. The standard method for diagnosis of schistosomiasis is detection of parasite eggs from patient samples. However, this method is not sufficient to detect asymptomatic patients, low egg numbers, or early infection. Therefore, diagnostic methods with higher sensitivity at the early stage of the disease are needed to fill this gap. The aim of this study was to identify potential biomarkers of early schistosomiasis using an untargeted metabolomics approach. Serum of uninfected and S. mekongi-infected mice was collected at 2, 4, and 8 weeks post-infection. Samples were extracted for metabolites and analyzed with a liquid chromatography-tandem mass spectrometer. Metabolites were annotated with the MS-DIAL platform and analyzed with Metaboanalyst bioinformatic tools. Multivariate analysis distinguished between metabolites from the different experimental conditions. Biomarker screening was performed using three methods: correlation coefficient analysis; feature important detection with a random forest algorithm; and receiver operating characteristic (ROC) curve analysis. Three compounds were identified as potential biomarkers at the early stage of the disease: heptadecanoyl ethanolamide; picrotin; and theophylline. The levels of these three compounds changed significantly during early-stage infection, and therefore these molecules may be promising schistosomiasis markers. These findings may help to improve early diagnosis of schistosomiasis, thus reducing the burden on patients and limiting spread of the disease in endemic areas.
Institute
Princess Srisavangavadhana College of Medicine, Chulabhorn Royal Academy
Last NameChienwichai
First NamePeerut
Address906, Kamphaeng Phet 6 Rd., Lak Si, Bangkok, 10210, Thailand
Emailpeerut.chi@cra.ac.th
Phone+6681687460
Submit Date2022-03-31
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2022-06-01
Release Version1
Peerut Chienwichai Peerut Chienwichai
https://dx.doi.org/10.21228/M8H691
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR001371
Project DOI:doi: 10.21228/M8H691
Project Title:Metabolomic profiles of S. mekongi-infected mouse serum at 0, 2, 4, 8 weeks (Positive mode)
Project Summary:Serum of uninfected and S. mekongi-infected mice was collected at 2, 4, and 8 weeks post-infection. Samples were extracted for metabolites and analyzed with a liquid chromatography-tandem mass spectrometer.
Institute:Princess Srisavangavadhana College of Medicine, Chulabhorn Royal Academy
Last Name:Chienwichai
First Name:Peerut
Address:906, Kamphaeng Phet 6 Rd., Lak Si, Bangkok, 10210, Thailand
Email:peerut.chi@cra.ac.th
Phone:+6681687460

Subject:

Subject ID:SU002244
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Experimental factor
SA2069762-Week post-infection 12 weeks after infection
SA2069772-Week post-infection 32 weeks after infection
SA2069782-Week post-infection 52 weeks after infection
SA2069792-Week post-infection 22 weeks after infection
SA2069802-Week post-infection 42 weeks after infection
SA2069814-Week post-infection 34 weeks after infection
SA2069824-Week post-infection 54 weeks after infection
SA2069834-Week post-infection 44 weeks after infection
SA2069844-Week post-infection 24 weeks after infection
SA2069854-Week post-infection 14 weeks after infection
SA2069868-Week post-infection 38 weeks after infection
SA2069878-Week post-infection 48 weeks after infection
SA2069888-Week post-infection 58 weeks after infection
SA2069898-Week post-infection 28 weeks after infection
SA2069908-Week post-infection 18 weeks after infection
SA206991Control 2No infection
SA206992Control 5No infection
SA206993Control 4No infection
SA206994Control 3No infection
SA206995Control 1No infection
Showing results 1 to 20 of 20

Collection:

Collection ID:CO002237
Collection Summary:5 Mice were infected with S. mekongi and serum samples were collected at 0, 2, 4, and 8 weeks after infection. Metabolite profiling was performed with mass spectrometer.
Sample Type:Blood (serum)

Treatment:

Treatment ID:TR002256
Treatment Summary:Blood was collected at 0, 2,4, and 8 weeks after infection

Sample Preparation:

Sampleprep ID:SP002250
Sampleprep Summary:20 μL serum was mixed with 80 μL cold methanol and vortexed for 1 minute. This mixture was then incubated at 4°C for 20 minutes and centrifuged at 12,000 rpm for 10 minutes. Next, the supernatant was collected and dried with a speed vacuum (Tomy Digital Biology, Tokyo, Japan). Samples were stored at −80°C until further analysis

Combined analysis:

Analysis ID AN003533 AN003534
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Agilent 1260 Agilent 1260
Column Waters Acquity BEH C8 (100 x 2.1mm, 1.7um) Waters Acquity BEH C8 (100 x 2.1mm, 1.7um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name ABI Sciex 5600+ TripleTOF ABI Sciex 5600+ TripleTOF
Ion Mode POSITIVE NEGATIVE
Units m/z m/z

Chromatography:

Chromatography ID:CH002610
Instrument Name:Agilent 1260
Column Name:Waters Acquity BEH C8 (100 x 2.1mm, 1.7um)
Chromatography Type:Reversed phase

MS:

MS ID:MS003291
Analysis ID:AN003533
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:Information-dependent acquisition mode composed of a TOF-MS scan and 10 dependent product ion scans were used in the high sensitivity mode with dynamic background subtraction. The mass range of the TOF-MS scan was m/z 100–1,000 and the product ion scan was set to m/z 50−1,000. Equal aliquots of each metabolite sample were pooled to form the quality control (QC) samples. The QC samples were injected before, during, and after sample analysis to assess the system performance.
Ion Mode:POSITIVE
  
MS ID:MS003292
Analysis ID:AN003534
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:Information-dependent acquisition mode composed of a TOF-MS scan and 10 dependent product ion scans were used in the high sensitivity mode with dynamic background subtraction. The mass range of the TOF-MS scan was m/z 100–1,000 and the product ion scan was set to m/z 50−1,000. Equal aliquots of each metabolite sample were pooled to form the quality control (QC) samples. The QC samples were injected before, during, and after sample analysis to assess the system performance.
Ion Mode:NEGATIVE
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