Summary of Study ST001960

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001247. The data can be accessed directly via it's Project DOI: 10.21228/M8HT3Z This work is supported by NIH grant, U2C- DK119886.

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Study IDST001960
Study TitleLipidomics of brown adipocytes treated with d9-choline
Study Typetargeted LC-QTOF/MS lipidomic profiling
Study SummaryBrown adipose tissue (BAT) was initially characterised as a thermogenic organ, and recent studies have suggested it plays a crucial role in maintaining systemic metabolic health. In this project, we demonstrated that alteration of BAT function contributes to development of heart failure through disorientation in choline metabolism. To analyze the detail effect of choline accumulation on brown adipocytes, we conducted the LC-QTOF/MS analysis using cultured brown adipocytes treated with d9-choline. In brown adipocytes treated with d9-choline, we found increase of phosphatidylcholine and lysophosphatidylcholine, suggesting that choline was metabolized in healthy brown adipocytes.
Institute
Juntendo University
DepartmentDepartment of Cardiovascular Biology and Medicine
Last NameYoshida
First NameYohko
Address2-1-1, Hongo, Bunkyo-ku, Tokyo, Tokyo, 1138421, Japan
Emailyohko105@yahoo.co.jp
Phone+81-3-3813-3111
Submit Date2021-10-14
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2022-11-01
Release Version1
Yohko Yoshida Yohko Yoshida
https://dx.doi.org/10.21228/M8HT3Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001247
Project DOI:doi: 10.21228/M8HT3Z
Project Title:Lipidomics of brown adipocytes treated with d9-choline
Project Type:targeted LC-QTOF/MS lipidomic profiling
Project Summary:Brown adipose tissue (BAT) was initially characterised as a thermogenic organ, and recent studies have suggested it plays a crucial role in maintaining systemic metabolic health. In this project, we demonstrated that alteration of BAT function contributes to development of heart failure through disorientation in choline metabolism. To analyze the detail effect of choline accumulation on brown adipocytes, we conducted the LC-QTOF/MS analysis using cultured brown adipocytes treated with d9-choline. In brown adipocytes treated with d9-choline, we found increase of phosphatidylcholine and lysophosphatidylcholine, suggesting that choline was metabolized in healthy brown adipocytes.
Institute:Juntendo University
Department:Department of Cardiovascular Biology and Medicine
Last Name:Yoshida
First Name:Yohko
Address:2-1-1, Hongo, Bunkyo-ku, Tokyo, Tokyo, 1138421, Japan
Email:yohko105@yahoo.co.jp
Phone:+81-3-3813-3111

Subject:

Subject ID:SU002040
Subject Type:Cultured cells
Subject Species:Mus musculus
Taxonomy ID:10090
Gender:Not applicable

Factors:

Subject type: Cultured cells; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Sample detail
SA1846041H-3cultured brown adipocytes treated with 1mM of d9-choline for 1hr
SA1846051H-4cultured brown adipocytes treated with 1mM of d9-choline for 1hr
SA1846061H-2cultured brown adipocytes treated with 1mM of d9-choline for 1hr
SA1846071H-1cultured brown adipocytes treated with 1mM of d9-choline for 1hr
SA18460824H-1cultured brown adipocytes treated with 1mM of d9-choline for 24hrs
SA18460924H-2cultured brown adipocytes treated with 1mM of d9-choline for 24hrs
SA18461024H-4cultured brown adipocytes treated with 1mM of d9-choline for 24hrs
SA18461124H-3cultured brown adipocytes treated with 1mM of d9-choline for 24hrs
SA1846126H-4cultured brown adipocytes treated with 1mM of d9-choline for 6hrs
SA1846136H-1cultured brown adipocytes treated with 1mM of d9-choline for 6hrs
SA1846146H-3cultured brown adipocytes treated with 1mM of d9-choline for 6hrs
SA1846156H-2cultured brown adipocytes treated with 1mM of d9-choline for 6hrs
SA184616Cont-2cultured brown adipocytes treated without d9-choline
SA184617Cont-3cultured brown adipocytes treated without d9-choline
SA184618Cont-1cultured brown adipocytes treated without d9-choline
SA184619Cont-4cultured brown adipocytes treated without d9-choline
Showing results 1 to 16 of 16

Collection:

Collection ID:CO002033
Collection Summary:Cells were harvested at the indicated time points and washed three times with ice cold 5% mannitol.
Sample Type:Cultured cells

Treatment:

Treatment ID:TR002052
Treatment Summary:Fully differentiated cultured brown adipocytes were treated with or without 1mM of d9-choline for 1, 6 or 24 hours.

Sample Preparation:

Sampleprep ID:SP002046
Sampleprep Summary:Lipids were extracted using a methanol–chloroform–water extraction method. The methanol used contained 25 μM each of methionine sulfone and D-camphor-10-sulphonic acid and 10 μM each of 1,2-dimyristoyl(d54)-sn-glycero-3-phosphocholine and 1,2-dihexanoyl(d22)-sn-glycero-3-phosphocholine.

Combined analysis:

Analysis ID AN003195
Analysis type MS
Chromatography type None (Direct infusion)
Chromatography system none
Column none
MS Type ESI
MS instrument type QTOF
MS instrument name Agilent 6530 QTOF
Ion Mode POSITIVE
Units fmol/cell

Chromatography:

Chromatography ID:CH002363
Instrument Name:none
Column Name:none
Chromatography Type:None (Direct infusion)

MS:

MS ID:MS002973
Analysis ID:AN003195
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:Mass spectra were acquired at the rate of 1.5 cycles /sec over an mass range of m/z100–1,700. The reference masses of reference standards were 121.050873(Purine) and 922.009798(HP-0921) for positive ion mode, and 112.985587(TFA) and 1033.988109(HP-0921+TFA) for negative ion mode. Raw data from LC–TOFMS were processed using Master Hands ver. 2.17.0.8 software for the quantification of lipids.
Ion Mode:POSITIVE
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