Summary of Study ST001781

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001113. The data can be accessed directly via it's Project DOI: 10.21228/M8TX2D This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001781
Study TitleIdentifying Candidate Differential Metabolites in lethal chlorpromazine poisoning Relative to non-drug related deaths (part III)
Study Typeoriginal study
Study Summarycandidate differential metabolites in lethal chlorpromazine poisoning Relative to non-drug related deaths (cervical dislocation, drowning, mechanical asphyxia and acute hemorrhagic shock).
Institute
Hebei medical university
Last NameBai
First NameRui
AddressNo.9 Tiyu North Street, Chang'an District
Email15822925144@163.com
Phone18522889554
Submit Date2021-04-04
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2025-03-30
Release Version1
Rui Bai Rui Bai
https://dx.doi.org/10.21228/M8TX2D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001113
Project DOI:doi: 10.21228/M8TX2D
Project Title:Examination of Candidate Differential Metabolites for Lethal Chlorpromazine Poisoning Using a LC-MS-based Metabolomics Approach in Mice
Project Type:Original Research
Project Summary:With the innovation of metabolite detection technology and the application of chemometrics in biomarker identification, metabolomics has been widely used to identify endogenous differential metabolites after drug poisoning. In this study, ultra-performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS) was combined with advanced chemometric approaches to identify differential metabolites that are associated with lethal chlorpromazine (CPZ) poisoning and associated perturbed metabolic pathways. Differential metabolite specificity and stability were assessed by comparing the CPZ samples to other antipsychotics, such as perphenazine (PER), clozapine (CLO) and olanzapine (OLA), that are associated with a high fatality frequency and by comparing them to non-drug related deaths (NDRDs) associated with hypoxia. The results implicated three candidate differential metabolites (acetyl-L-carnitine, propionyl-L-carnitine and succinic acid) with a high and relatively stable sensitivity (85.7–100%) following predictive analysis. Additionally, no differential metabolites were identified when comparing CPZ to PRE, OLA and CLO, but all of the drugs showed a similar pharmacodynamic receptor profile. Overall, this study provides a methodological and theoretical basis for biomarker identification in lethal CPZ poisoning (LCP) cases.
Institute:new
Last Name:Bai
First Name:rui
Address:Hebei Province, Shijiazhuang 050018, P.R. China
Email:15822925144@163.com
Phone:18522889554

Subject:

Subject ID:SU001858
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Age Or Age Range:7-8 weeks
Gender:Male and female
Species Group:Mammals

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Phenotype 1 Phenotype 2
SA165099F-T-5Cervical dislocation Non-drug related death
SA165100F-T-4Cervical dislocation Non-drug related death
SA165101F-T-2Cervical dislocation Non-drug related death
SA165102F-T-6Cervical dislocation Non-drug related death
SA165103F-T-3Cervical dislocation Non-drug related death
SA165104M-T-3Cervical dislocation Non-drug related death
SA165105M-T-6Cervical dislocation Non-drug related death
SA165106M-T-5Cervical dislocation Non-drug related death
SA165107M-T-4Cervical dislocation Non-drug related death
SA165108F-T-1Cervical dislocation Non-drug related death
SA165109M-T-2Cervical dislocation Non-drug related death
SA165110M-T-1Cervical dislocation Non-drug related death
SA165111M-N-2Drowning Non-drug related death
SA165112F-N-1Drowning Non-drug related death
SA165113M-N-3Drowning Non-drug related death
SA165114M-N-4Drowning Non-drug related death
SA165115M-N-5Drowning Non-drug related death
SA165116M-N-1Drowning Non-drug related death
SA165117F-N-6Drowning Non-drug related death
SA165118F-N-2Drowning Non-drug related death
SA165119F-N-3Drowning Non-drug related death
SA165120F-N-4Drowning Non-drug related death
SA165121F-N-5Drowning Non-drug related death
SA165122M-N-6Drowning Non-drug related death
SA165123M-X-3Hemorrhagic shock Non-drug related death
SA165124F-X-3Hemorrhagic shock Non-drug related death
SA165125F-X-4Hemorrhagic shock Non-drug related death
SA165126F-X-5Hemorrhagic shock Non-drug related death
SA165127F-X-6Hemorrhagic shock Non-drug related death
SA165128F-X-2Hemorrhagic shock Non-drug related death
SA165129F-X-1Hemorrhagic shock Non-drug related death
SA165130M-X-4Hemorrhagic shock Non-drug related death
SA165131M-X-5Hemorrhagic shock Non-drug related death
SA165132M-X-6Hemorrhagic shock Non-drug related death
SA165133M-X-2Hemorrhagic shock Non-drug related death
SA165134M-X-1Hemorrhagic shock Non-drug related death
SA165135M-S-5Lethal chlorpromazine poisoning Lethal chlorpromazine poisoning
SA165136F-S-1Lethal chlorpromazine poisoning Lethal chlorpromazine poisoning
SA165137M-S-4Lethal chlorpromazine poisoning Lethal chlorpromazine poisoning
SA165138M-S-2Lethal chlorpromazine poisoning Lethal chlorpromazine poisoning
SA165139M-S-3Lethal chlorpromazine poisoning Lethal chlorpromazine poisoning
SA165140F-S-2Lethal chlorpromazine poisoning Lethal chlorpromazine poisoning
SA165141M-S-6Lethal chlorpromazine poisoning Lethal chlorpromazine poisoning
SA165142F-S-3Lethal chlorpromazine poisoning Lethal chlorpromazine poisoning
SA165143M-S-1Lethal chlorpromazine poisoning Lethal chlorpromazine poisoning
SA165144F-S-5Lethal chlorpromazine poisoning Lethal chlorpromazine poisoning
SA165145F-S-6Lethal chlorpromazine poisoning Lethal chlorpromazine poisoning
SA165146F-S-4Lethal chlorpromazine poisoning Lethal chlorpromazine poisoning
SA165147M-Z-3Mechanical asphyxia Non-drug related death
SA165148M-Z-4Mechanical asphyxia Non-drug related death
SA165149M-Z-6Mechanical asphyxia Non-drug related death
SA165150M-Z-2Mechanical asphyxia Non-drug related death
SA165151M-Z-5Mechanical asphyxia Non-drug related death
SA165152F-Z-1Mechanical asphyxia Non-drug related death
SA165153F-Z-3Mechanical asphyxia Non-drug related death
SA165154F-Z-2Mechanical asphyxia Non-drug related death
SA165155F-Z-4Mechanical asphyxia Non-drug related death
SA165156F-Z-5Mechanical asphyxia Non-drug related death
SA165157F-Z-6Mechanical asphyxia Non-drug related death
SA165158M-Z-1Mechanical asphyxia Non-drug related death
Showing results 1 to 60 of 60

Collection:

Collection ID:CO001851
Collection Summary:plasma were isolated and then flash-frozen in liquid N2
Collection Protocol Filename:process_protocol.pdf
Sample Type:Blood (plasma)

Treatment:

Treatment ID:TR001871
Treatment Summary:Lethal poisoning animals were given chlorpromzine through gavage.Non-drug related death animal models according to previous study.Abdominal aortic blood was obtained after death.
Treatment Protocol Filename:process_protocol.pdf
Treatment:drug
Treatment Compound:chlorpromazine
Treatment Route:gavage
Treatment Dose:150mg/100g
Treatment Dosevolume:300ul
Treatment Doseduration:once
Treatment Vehicle:Gavage needle
Animal Acclimation Duration:one week
Animal Fasting:one night before experiment
Animal Endp Clinical Signs:stop breathing

Sample Preparation:

Sampleprep ID:SP001864
Sampleprep Summary:100 μL of plasma or 70 μL of whole blood were added to tubes with ice-cold methanol (Vsample: Vextraction = 1:3). Each sample was then vortexed for 30 s, sonicated for 10 min in an ice-water bath, and incubated for 20 min at −20°C to allow protein precipitation. The mixtures were then centrifuged at 12,000 g for 10 min at 4°C.
Processing Storage Conditions:On ice
Extract Storage:On ice

Combined analysis:

Analysis ID AN002892
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Dionex Ultimate 3000
Column Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive Orbitrap
Ion Mode UNSPECIFIED
Units peak area

Chromatography:

Chromatography ID:CH002144
Chromatography Summary:Separation was performed using a reverse-phase (C18) column (HSS T3 column; 2.1 mm × 100 mm, 1.8 μm; Waters), with a gradient elution of solution A (0.1% formic acid in water) and solution B (acetonitrile) of 0.3 mL/min and an injection volume of 5 μL. The elution gradient was set as follows: 0 min, 98% A; 1 min, 98% A; 12 min, 2% A; 16 min, 2% A; 16.1 min, 98% A; and 20 min, 98% A.
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um)
Column Temperature:40
Flow Rate:300ul/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile
Analytical Time:20min
Chromatography Type:Reversed phase

MS:

MS ID:MS002685
Analysis ID:AN002892
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:compound discovery 3.1
Ion Mode:UNSPECIFIED
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