Summary of Study ST001739

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001113. The data can be accessed directly via it's Project DOI: 10.21228/M8TX2D This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001739
Study TitleDifferential Metabolites and Disturbed Metabolic Pathways Associated with chlorpromazine Poisoning
Study SummaryPerturbed pathways and differential metabolites analysis for lethal chlorpromzaine poisoning relative to control and non-lethal chlorpromazine poisoning group.
Institute
Hebei medical university
Last NameBai
First NameRui
AddressNo.9 Tiyu North Street, Chang'an District
Email15822925144@163.com
Phone18522889554
Submit Date2021-03-29
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2025-03-30
Release Version1
Rui Bai Rui Bai
https://dx.doi.org/10.21228/M8TX2D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001113
Project DOI:doi: 10.21228/M8TX2D
Project Title:Examination of Candidate Differential Metabolites for Lethal Chlorpromazine Poisoning Using a LC-MS-based Metabolomics Approach in Mice
Project Type:Original Research
Project Summary:With the innovation of metabolite detection technology and the application of chemometrics in biomarker identification, metabolomics has been widely used to identify endogenous differential metabolites after drug poisoning. In this study, ultra-performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS) was combined with advanced chemometric approaches to identify differential metabolites that are associated with lethal chlorpromazine (CPZ) poisoning and associated perturbed metabolic pathways. Differential metabolite specificity and stability were assessed by comparing the CPZ samples to other antipsychotics, such as perphenazine (PER), clozapine (CLO) and olanzapine (OLA), that are associated with a high fatality frequency and by comparing them to non-drug related deaths (NDRDs) associated with hypoxia. The results implicated three candidate differential metabolites (acetyl-L-carnitine, propionyl-L-carnitine and succinic acid) with a high and relatively stable sensitivity (85.7–100%) following predictive analysis. Additionally, no differential metabolites were identified when comparing CPZ to PRE, OLA and CLO, but all of the drugs showed a similar pharmacodynamic receptor profile. Overall, this study provides a methodological and theoretical basis for biomarker identification in lethal CPZ poisoning (LCP) cases.
Institute:new
Last Name:Bai
First Name:rui
Address:Hebei Province, Shijiazhuang 050018, P.R. China
Email:15822925144@163.com
Phone:18522889554

Subject:

Subject ID:SU001816
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Age Or Age Range:7-8 weeks
Gender:Male and female
Species Group:Mammals

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Phenotype
SA162944MT2Control
SA162945MT3Control
SA162946MT4Control
SA162947MT1Control
SA162948FT5Control
SA162949FT1Control
SA162950FT3Control
SA162951FT4Control
SA162952MT5Control
SA162953FT2Control
SA162954MCS1Lethal chlorpromazine poisoning
SA162955FCS5Lethal chlorpromazine poisoning
SA162956FCS4Lethal chlorpromazine poisoning
SA162957FCS3Lethal chlorpromazine poisoning
SA162958MCS2Lethal chlorpromazine poisoning
SA162959FCS1Lethal chlorpromazine poisoning
SA162960MCS5Lethal chlorpromazine poisoning
SA162961MCS4Lethal chlorpromazine poisoning
SA162962MCS3Lethal chlorpromazine poisoning
SA162963FCS2Lethal chlorpromazine poisoning
SA162964MCWS1Non-lethal chlorpromazine poisoning
SA162965FCWS3Non-lethal chlorpromazine poisoning
SA162966FCWS2Non-lethal chlorpromazine poisoning
SA162967FCWS1Non-lethal chlorpromazine poisoning
SA162968FCWS4Non-lethal chlorpromazine poisoning
SA162969FCWS5Non-lethal chlorpromazine poisoning
SA162970MCWS4Non-lethal chlorpromazine poisoning
SA162971MCWS3Non-lethal chlorpromazine poisoning
SA162972MCWS2Non-lethal chlorpromazine poisoning
SA162973MCWS5Non-lethal chlorpromazine poisoning
Showing results 1 to 30 of 30

Collection:

Collection ID:CO001809
Collection Summary:plasma were isolated and then flash-frozen in liquid N2
Collection Protocol Filename:process_protocol.pdf
Sample Type:Blood (plasma)

Treatment:

Treatment ID:TR001829
Treatment Summary:Lethal poisoning and non-lethal poisoning animals were given same dose of chlorpromzine through gavage.Abdominal aortic blood was obtained after death or poisoning.
Treatment Protocol Filename:process_protocol.pdf
Treatment:drug
Treatment Compound:chlorpromazine
Treatment Route:gavage
Treatment Dose:150mg/100g
Treatment Dosevolume:300ul
Treatment Doseduration:once
Treatment Vehicle:Gavage needle
Animal Acclimation Duration:one week
Animal Fasting:one night before experiment
Animal Endp Clinical Signs:stop breathing

Sample Preparation:

Sampleprep ID:SP001822
Sampleprep Summary:100 μL of plasma or 70 μL of whole blood were added to tubes with ice-cold methanol (Vsample: Vextraction = 1:3). Each sample was then vortexed for 30 s, sonicated for 10 min in an ice-water bath, and incubated for 20 min at −20°C to allow protein precipitation. The mixtures were then centrifuged at 12,000 g for 10 min at 4°C.
Processing Storage Conditions:On ice
Extract Storage:On ice

Combined analysis:

Analysis ID AN002832
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Dionex Ultimate 3000
Column Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive Orbitrap
Ion Mode UNSPECIFIED
Units peak area

Chromatography:

Chromatography ID:CH002095
Chromatography Summary:Separation was performed using a reverse-phase (C18) column (HSS T3 column; 2.1 mm × 100 mm, 1.8 μm; Waters), with a gradient elution of solution A (0.1% formic acid in water) and solution B (acetonitrile) of 0.3 mL/min and an injection volume of 5 μL. The elution gradient was set as follows: 0 min, 98% A; 1 min, 98% A; 12 min, 2% A; 16 min, 2% A; 16.1 min, 98% A; and 20 min, 98% A.
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um)
Column Temperature:40
Flow Rate:300ul/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile
Analytical Time:20min
Chromatography Type:Reversed phase

MS:

MS ID:MS002625
Analysis ID:AN002832
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:compound discovery 3.1
Ion Mode:UNSPECIFIED
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