Summary of Study ST000638

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000457. The data can be accessed directly via it's Project DOI: 10.21228/M8060Z This work is supported by NIH grant, U2C- DK119886.


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Study IDST000638
Study TitleAmino Acid Metabolites of Dietary Salt Effects on Blood Pressure in Rat Urine and Kidney Tissue (part XI)
Study SummaryWe propose to analyze kidney tissue extract and urine samples from SS and SS.Fh1+ transgenic rats in addition to the analysis of urine samples from the DASH2 trial. The analysis of the rat samples will be highly valuable for several reasons. First, it will to take the findings in human subjects back to animal models and prepare us for further mechanistic studies. We hypothesize at least some of the effects of dietary salt intake on metabolite profiles in human will be recapitulated or altered in the SS rat. If this is confirmed, we will have a highly informative animal model ready for mechanistic studies in which we can investigate the functional contribution of specific metabolites to hypertension and the mechanisms involved. Second, the rat study will allow us to take advantage of a new and unique transgenic SS.Fh1+ model that we recently developed that overexpresses fumarase (Fh1) on the genetic background of the SS rat. Fumarase is a TCA cycle enzyme previously implicated in salt-induced hypertension in SS rats.
Mayo Clinic
Last NameLiang
First NameMingyu
AddressMedical College of Wisconsin 8701 Watertown Plank Road Milwaukee, WI 53226
Submit Date2017-06-23
Analysis Type DetailLC-MS
Release Date2019-07-17
Release Version1
Mingyu Liang Mingyu Liang application/zip

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Project ID:PR000457
Project DOI:doi: 10.21228/M8060Z
Project Title:Metabolomic Mechanisms of Dietary Salt Effects on Blood Pressure
Project Summary:Enhanced sensitivity of blood pressure to salt intake is observed in approximately 50% of hypertensive patients, reaching 75% in African American hypertensive patients. We recently discovered a novel role of abnormal cellular intermediary metabolism in hypertension in the Dahl salt-sensitive (SS) rat, the most commonly used polygenic, hereditary model of human salt-sensitive hypertension. We propose to test the hypothesis that blood pressure sensitivity to dietary salt intake in human is associated with metabolite changes in the urine. Leveraging the expertise and resources at the Mayo Clinic Metabolomics Resources Core, we propose to perform targeted LC/MS analysis and NMR spectra generation in urine samples obtained from a subset of subjects from the Dietary Approaches to Stop Hypertension – Sodium (DASH2) clinical trial and kidney tissue extract and urine samples from SS rats and a newly generated transgenic rat that overexpresses fumarase (SS.Fh1+). The study will be the first to systematically characterize urinary metabolite profiles associated with blood pressure response to salt in humans. The study is anticipated to generate new insight into the mechanisms (particularly renal mechanisms) underlying salt-sensitive hypertension. Findings of the proposed study could lead to an expanded clinical study as well as mechanistic studies in animal models.
Institute:Mayo Clinic
Last Name:Liang
First Name:Mingyu
Address:Medical College of Wisconsin 8701 Watertown Plank Road Milwaukee, WI 53226


Subject ID:SU000661
Subject Type:Rat
Subject Species:Rattus norvegicus
Taxonomy ID:10116
Species Group:Mammal


Subject type: Rat; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id sample ID group
SA035975ms6104-17YCR1 kidney cortex Transgenic Het
SA035976ms6104-23YCR1 kidney cortex Transgenic Het
SA035977ms6104-21YCR1 kidney cortex Transgenic Het
SA035978ms6104-24YCR1 kidney cortex Transgenic Het
SA035979ms6104-18YCR1 kidney cortex Transgenic Negative
SA035980ms6104-22YCR1 kidney cortex Transgenic Negative
SA035981ms6104-19YCR1 kidney cortex Transgenic Negative
SA035982ms6104-20YCR1 kidney cortex Transgenic Negative
SA035983ms6104-16YCR1 outer medulla kidney Transgenic Het
SA035984ms6104-15YCR1 outer medulla kidney Transgenic Het
SA035985ms6104-13YCR1 outer medulla kidney Transgenic Het
SA035986ms6104-9YCR1 outer medulla kidney Transgenic Het
SA035987ms6104-10YCR1 outer medulla kidney Transgenic Negative
SA035988ms6104-11YCR1 outer medulla kidney Transgenic Negative
SA035989ms6104-12YCR1 outer medulla kidney Transgenic Negative
SA035990ms6104-14YCR1 outer medulla kidney Transgenic Negative
SA035991ms6104-1YCR1 urine Transgenic Het
SA035992ms6104-2YCR2 urine Transgenic Negative
SA035993ms6104-3YCR3 urine Transgenic Negative
SA035994ms6104-4YCR4 urine Transgenic Negative
SA035995ms6104-5YCR5 urine Transgenic Het
SA035996ms6104-6YCR6 urine Transgenic Negative
SA035997ms6104-7YCR7 urine Transgenic Het
SA035998ms6104-8YCR8 urine Transgenic Het
Showing results 1 to 24 of 24


Collection ID:CO000655
Collection Summary:Samples are from Transgenic fumerase rats and WT littermates, 13 weeks of age, placed on low salt or high salt dyets. We are interested in any differences in the TCA and AA intermediates.
Sample Type:Kidney


Treatment ID:TR000675
Treatment Summary:We will analyze kidney tissue extract and urine samples from SS rats and the newly generated SS.Fh1+ transgenic rats. The SS.Fh1+ rat was generated using a Sleeping Beauty transposon-mediated transgenic technique. Overexpression of fumarase has been confirmed in SS.Fh1+ rats. Preliminary study indicated that the development of salt-induced hypertension was altered in SS.Fh1+ rats compared to wild-type littermate SS rats. We will analyze kidney tissue extract and urine samples collected from rats maintained on a 0.4% NaCl diet or switched to a 4% NaCl diet for 7 days. The dietary protocol has been used in numerous studies to examine mechanisms underlying salt sensitivity including early responses (7 days) to salt in the SS model. In total, 64 rat samples (two rat strains, two salt levels, and 8 rats per condition) will be analyzed.

Sample Preparation:

Sampleprep ID:SP000668
Sampleprep Summary:Rat urine and kidney tissue extract (outer medulla and cortex) Amino Acid concentrations. Urine AA concentrations in uM and tissue in nmol/vial

Combined analysis:

Analysis ID AN000970
Analysis type MS
Chromatography type Reversed phase
Chromatography system Waters Acquity
Column Waters Acquity BEH C18 (150 x 2.1mm,1.7um)
MS instrument type Triple quadrupole
MS instrument name Thermo Quantum Ultra
Units urine uM and Tissue nnol/vial


Chromatography ID:CH000695
Instrument Name:Waters Acquity
Column Name:Waters Acquity BEH C18 (150 x 2.1mm,1.7um)
Chromatography Type:Reversed phase


MS ID:MS000865
Analysis ID:AN000970
Instrument Name:Thermo Quantum Ultra
Instrument Type:Triple quadrupole