Summary of Study ST000637

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000457. The data can be accessed directly via it's Project DOI: 10.21228/M8060Z This work is supported by NIH grant, U2C- DK119886.


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Study IDST000637
Study TitleTCA Cycle Metabolites of Dietary Salt Effects on Blood Pressure in Rat Urine and Kidney Tissue (part X)
Study SummaryWe propose to analyze kidney tissue extract and urine samples from SS and SS.Fh1+ transgenic rats in addition to the analysis of urine samples from the DASH2 trial. The analysis of the rat samples will be highly valuable for several reasons. First, it will to take the findings in human subjects back to animal models and prepare us for further mechanistic studies. We hypothesize at least some of the effects of dietary salt intake on metabolite profiles in human will be recapitulated or altered in the SS rat. If this is confirmed, we will have a highly informative animal model ready for mechanistic studies in which we can investigate the functional contribution of specific metabolites to hypertension and the mechanisms involved. Second, the rat study will allow us to take advantage of a new and unique transgenic SS.Fh1+ model that we recently developed that overexpresses fumarase (Fh1) on the genetic background of the SS rat. Fumarase is a TCA cycle enzyme previously implicated in salt-induced hypertension in SS rats.
Mayo Clinic
Last NameLiang
First NameMingyu
AddressMedical College of Wisconsin 8701 Watertown Plank Road Milwaukee, WI 53226
Submit Date2017-06-23
Analysis Type DetailGC-MS
Release Date2019-07-17
Release Version1
Mingyu Liang Mingyu Liang application/zip

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Project ID:PR000457
Project DOI:doi: 10.21228/M8060Z
Project Title:Metabolomic Mechanisms of Dietary Salt Effects on Blood Pressure
Project Summary:Enhanced sensitivity of blood pressure to salt intake is observed in approximately 50% of hypertensive patients, reaching 75% in African American hypertensive patients. We recently discovered a novel role of abnormal cellular intermediary metabolism in hypertension in the Dahl salt-sensitive (SS) rat, the most commonly used polygenic, hereditary model of human salt-sensitive hypertension. We propose to test the hypothesis that blood pressure sensitivity to dietary salt intake in human is associated with metabolite changes in the urine. Leveraging the expertise and resources at the Mayo Clinic Metabolomics Resources Core, we propose to perform targeted LC/MS analysis and NMR spectra generation in urine samples obtained from a subset of subjects from the Dietary Approaches to Stop Hypertension – Sodium (DASH2) clinical trial and kidney tissue extract and urine samples from SS rats and a newly generated transgenic rat that overexpresses fumarase (SS.Fh1+). The study will be the first to systematically characterize urinary metabolite profiles associated with blood pressure response to salt in humans. The study is anticipated to generate new insight into the mechanisms (particularly renal mechanisms) underlying salt-sensitive hypertension. Findings of the proposed study could lead to an expanded clinical study as well as mechanistic studies in animal models.
Institute:Mayo Clinic
Last Name:Liang
First Name:Mingyu
Address:Medical College of Wisconsin 8701 Watertown Plank Road Milwaukee, WI 53226


Subject ID:SU000660
Subject Type:Rat
Subject Species:Rattus norvegicus
Taxonomy ID:10116
Species Group:Mammal


Subject type: Rat; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id sample source group
SA035951ms6103-17YCR1 kidney cortex Transgenic Het
SA035952ms6103-23YCR1 kidney cortex Transgenic Het
SA035953ms6103-21YCR1 kidney cortex Transgenic Het
SA035954ms6103-24YCR1 kidney cortex Transgenic Het
SA035955ms6103-18YCR1 kidney cortex Transgenic Negative
SA035956ms6103-22YCR1 kidney cortex Transgenic Negative
SA035957ms6103-19YCR1 kidney cortex Transgenic Negative
SA035958ms6103-20YCR1 kidney cortex Transgenic Negative
SA035959ms6103-16YCR1 outer medulla kidney Transgenic Het
SA035960ms6103-15YCR1 outer medulla kidney Transgenic Het
SA035961ms6103-13YCR1 outer medulla kidney Transgenic Het
SA035962ms6103-9YCR1 outer medulla kidney Transgenic Het
SA035963ms6103-10YCR1 outer medulla kidney Transgenic Negative
SA035964ms6103-11YCR1 outer medulla kidney Transgenic Negative
SA035965ms6103-12YCR1 outer medulla kidney Transgenic Negative
SA035966ms6103-14YCR1 outer medulla kidney Transgenic Negative
SA035967ms6103-1YCR1 urine Transgenic Het
SA035968ms6103-2YCR2 urine Transgenic Negative
SA035969ms6103-3YCR3 urine Transgenic Negative
SA035970ms6103-4YCR4 urine Transgenic Negative
SA035971ms6103-5YCR5 urine Transgenic Het
SA035972ms6103-6YCR6 urine Transgenic Negative
SA035973ms6103-7YCR7 urine Transgenic Het
SA035974ms6103-8YCR8 urine Transgenic Het
Showing results 1 to 24 of 24


Collection ID:CO000654
Collection Summary:The objective of the study is to identify Fumarase in hypertension. Samples are from Transgenic fumerase rats and WT littermates, 13 weeks of age
Sample Type:Kidney


Treatment ID:TR000674
Treatment Summary:We will analyze kidney tissue extract and urine samples from SS rats and the newly generated SS.Fh1+ transgenic rats. The SS.Fh1+ rat was generated using a Sleeping Beauty transposon-mediated transgenic technique. Overexpression of fumarase has been confirmed in SS.Fh1+ rats. Preliminary study indicated that the development of salt-induced hypertension was altered in SS.Fh1+ rats compared to wild-type littermate SS rats. We will analyze kidney tissue extract and urine samples collected from rats maintained on a 0.4% NaCl diet or switched to a 4% NaCl diet for 7 days. The dietary protocol has been used in numerous studies to examine mechanisms underlying salt sensitivity including early responses (7 days) to salt in the SS model. In total, 64 rat samples (two rat strains, two salt levels, and 8 rats per condition) will be analyzed.

Sample Preparation:

Sampleprep ID:SP000667
Sampleprep Summary:Rat urine and kidney tissue extract (outer medulla and cortex) TCA concentrations. Urine TCA concentrations in uM and tissue in nmol/vial

Combined analysis:

Analysis ID AN000969
Analysis type MS
Chromatography type GC
Chromatography system Agilent 7890B
Column Agilent HP5-MS (30m × 0.25mm, 0.25 um)
MS Type EI
MS instrument type Single quadrupole
MS instrument name Agilent 5975C
Units urine uM and Tissue nnol/vial


Chromatography ID:CH000694
Instrument Name:Agilent 7890B
Column Name:Agilent HP5-MS (30m × 0.25mm, 0.25 um)
Chromatography Type:GC


MS ID:MS000864
Analysis ID:AN000969
Instrument Name:Agilent 5975C
Instrument Type:Single quadrupole
MS Type:EI