Summary of Study ST000532
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000391. The data can be accessed directly via it's Project DOI: 10.21228/M8HG71 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000532 |
| Study Title | CHEAR Plasma Reference Material Plasma GC-MS |
| Study Type | Metabolomics |
| Study Summary | CHEAR Reference Material Plasma was provided by Emory University. The material was prepared and analyzed using the GC-TOF MS workflow of the Eastern Regional Metabolomics Resource Core (protocols available in metabolomics workbench). Seven samples were injected of the sample reference material prepared in replicate. |
| Institute | RTI International |
| Department | Discovery-Sciences-Technology (DST) |
| Laboratory | RTI CHEAR Analytical Hub - Untargeted Analysis Resource Core |
| Last Name | Fennell |
| First Name | Timothy |
| Address | 3040 E Cornwallis Road, Research Triangle Park, NC 27709 |
| fennell@rti.org | |
| Phone | 919-485-2781 |
| Submit Date | 2016-12-30 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | cdf |
| Chear Study | Yes |
| Analysis Type Detail | GC-MS |
| Release Date | 2024-12-31 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000391 |
| Project DOI: | doi: 10.21228/M8HG71 |
| Project Title: | CHEAR Plasma Reference Material ProficiencyTest GC-MS |
| Project Type: | CHEAR-UT-1 |
| Project Summary: | CHEAR Reference Material Plasma was provided by Emory University. The material was prepared and analyzed using the GC-TOF MS workflow of the Eastern Regional Metabolomics Resource Core (protocols available in metabolomics workbench). Seven samples were injected of the sample reference material prepared in replicate. |
| Institute: | RTI International |
| Department: | Discovery-Science-Technology |
| Laboratory: | RTI CHEAR Analytical Hub - Untargeted Analysis Resource Core (UARC) |
| Last Name: | Fennell |
| First Name: | Timothy |
| Address: | 3040 E Cornwallis Road, Research Triangle Park, NC 27709 |
| Email: | fennell@rti.org |
| Phone: | 919-485-2781 |
Subject:
| Subject ID: | SU000554 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Mammals |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | SAMPLE TYPE |
|---|---|---|
| SA027902 | CHE000054 0207a | Replicate |
| SA027903 | CHE000054 0208a | Replicate |
| SA027904 | CHE000054 0206a | Replicate |
| SA027905 | CHE000054 0205a | Replicate |
| SA027906 | CHE000054 0203a | Replicate |
| SA027907 | CHE000054 0204a | Replicate |
| SA027908 | CHE000054 0202a | Replicate |
| Showing results 1 to 7 of 7 |
Collection:
| Collection ID: | CO000548 |
| Collection Summary: | CHEAR Reference Material Plasma was provided by Emory University. |
| Sample Type: | Blood |
Treatment:
| Treatment ID: | TR000568 |
| Treatment Summary: | No treatment. |
Sample Preparation:
| Sampleprep ID: | SP000561 |
| Sampleprep Summary: | liquots of 30µL of plasma were mixed with 1000µL of (3:3:2) ACN:IPA:H2O by vortex mixer then centrifuged. Supernatant recovered. Archived 450µL of supernatant. Continued prep of remaining 450µL supernatant. Evaporated to dryness w/speed vac. Samples resuspended in 450µL of 1:1 ACN:H2O. Evaporated to dryness w/speed vac. Formation of methoximes by adding 10µL of 40mg/mL MeOx in pyridine to each sample. Placed onto Thermomixer for 90 min at 30⁰C. Then FAME retention index markers and MSTFA added to each sample for derivatization step. Samples returned to Thermomixer for 45 min at 70⁰C. |
Combined analysis:
| Analysis ID | AN000811 |
|---|---|
| Chromatography ID | CH000584 |
| MS ID | MS000718 |
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Leco Pegasus 4D GC |
| Column | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| MS Type | EI |
| MS instrument type | GC x GC-TOF |
| MS instrument name | Leco Pegasus 4D GCxGC TOF |
| Ion Mode | POSITIVE |
| Units | intensity |
Chromatography:
| Chromatography ID: | CH000584 |
| Instrument Name: | Leco Pegasus 4D GC |
| Column Name: | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| Column Pressure: | 10.4 psi |
| Flow Gradient: | Constant Flow |
| Flow Rate: | 1mL/min |
| Injection Temperature: | 250 degrees C |
| Retention Index: | FAME retention index markers C8-C30 |
| Sample Injection: | 0.5uL |
| Analytical Time: | 20 minutes |
| Transferline Temperature: | 280 degrees C |
| Chromatography Type: | GC |
MS:
| MS ID: | MS000718 |
| Analysis ID: | AN000811 |
| Instrument Name: | Leco Pegasus 4D GCxGC TOF |
| Instrument Type: | GC x GC-TOF |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
