Summary of Study ST000462

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000355. The data can be accessed directly via it's Project DOI: 10.21228/M85C88 This work is supported by NIH grant, U2C- DK119886.


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Study IDST000462
Study TitleCNS and peripheral metabolomics of calorie restriction in a mouse model of Alzheimer’s disease (part I)
Study SummaryThis pilot metabolomic study will evaluate brain specimens from an established mouse model of AD, the tq2576 mouse model of cerebral amyloid overexpression (APP), in comparison to their non-transgenic (NTG) littermates. These animals were either on a CR or ad libitum (AL) diet, and specimens were collected at two time points (5 and 15 months of age). Tissue from this cohorts of mice have already undergone microbiome analysis, and await coordinated brain and peripheral tissue assessments. Future analysis will include metabolomics, RNA-seq, and microarray data to assess the gut-brain microbiome system in neurodegenerative disorders.
University of North Carolina
LaboratorySumner Lab
Last NameSumner
First NameSusan
AddressEastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081
Submit Date2016-09-07
Raw Data AvailableYes
Raw Data File Type(s)1r
Analysis Type DetailNMR
Release Date2017-10-03
Release Version1
Susan Sumner Susan Sumner application/zip

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Project ID:PR000355
Project DOI:doi: 10.21228/M85C88
Project Title:CNS and peripheral metabolomics of calorie restriction in a mouse model of Alzheimer’s disease
Project Summary:Alzheimer’s disease (AD) is a devastating neurodegenerative disorder that robs people of their memory and cognitive function. Currently, no successful treatment or preventative measure exists for AD. Calorie restriction (CR) is a dietary regimen posited to suppress genetic programs of aging and reduce AD-related pathology. CR is known to enhance longevity and mitigate aging phenotypes in multiple model species. Mechanisms underlying the benefits of CR remain unknown, particularly in areas of the brain selectively vulnerable to age-related AD pathology such as the hippocampus, a region crucial for learning and memory. Moreover, AD pathology can be influenced by changes in diet, metabolism, and immunity, indicating that factors distant from the brain may play a role in pathogenesis. The intestinal microbiota, composed of trillions of microbial cells, influences host metabolism, immunity, and cognitive function, and is posited to be linked mechanistically to AD pathobiology, but a specific role remains to be adequately tested. We hypothesize that mechanisms underlying the benefits of CR are cell-type and organ specific, involving the gut-brain microbiome throughout the lifespan, this requiring subregional analysis in the brain as well as coordinated assessments of key peripheral targets including the liver, fecal pellets , and plasma. Thus, CR is proposed to be a viable treatment option that may ameliorate the development of AD-related pathology, and importantly, reveal mechanisms that attenuate age-related expression changes in vulnerable cells.
Institute:New York University
Department: Langone Medical Center
Last Name:Ginsberg
First Name:Stephen
Address:140 Old Orangeburg Road, Orangeburg, NY 10962


Subject ID:SU000483
Subject Type:Murine
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal


Subject type: Murine; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Age Genotype Gender Diet
SA023268B_1315 APP F AL
SA023269B_1115 APP F AL
SA023270B_1215 APP F AL
SA023271B_1415 APP F AL
SA023272B_1715 APP F CR
SA023273B_1815 APP F CR
SA023274B_1615 APP F CR
SA023275B_1515 APP F CR
SA023276B_4715 APP M AL
SA023277B_5015 APP M AL
SA023278B_4815 APP M AL
SA023279B_4915 APP M AL
SA023280B_5315 APP M CR
SA023281B_5215 APP M CR
SA023282B_5115 APP M CR
SA023283B_5415 APP M CR
SA023284B_3015 NTG F AL
SA023285B_3115 NTG F AL
SA023286B_3215 NTG F AL
SA023287B_2915 NTG F AL
SA023288B_3515 NTG F CR
SA023289B_3315 NTG F CR
SA023290B_3415 NTG F CR
SA023291B_3615 NTG F CR
SA023292B_6615 NTG M AL
SA023293B_6715 NTG M AL
SA023294B_6815 NTG M AL
SA023295B_6515 NTG M AL
SA023296B_7215 NTG M CR
SA023297B_7115 NTG M CR
SA023298B_6915 NTG M CR
SA023299B_7015 NTG M CR
SA023300B_55 APP F AL
SA023301B_45 APP F AL
SA023302B_25 APP F AL
SA023303B_15 APP F AL
SA023304B_35 APP F AL
SA023305B_85 APP F CR
SA023306B_105 APP F CR
SA023307B_65 APP F CR
SA023308B_75 APP F CR
SA023309B_95 APP F CR
SA023310B_415 APP M AL
SA023311B_375 APP M AL
SA023312B_385 APP M AL
SA023313B_395 APP M AL
SA023314B_405 APP M AL
SA023315B_465 APP M CR
SA023316B_435 APP M CR
SA023317B_425 APP M CR
SA023318B_445 APP M CR
SA023319B_455 APP M CR
SA023320B_205 NTG F AL
SA023321B_225 NTG F AL
SA023322B_235 NTG F AL
SA023323B_195 NTG F AL
SA023324B_215 NTG F AL
SA023325B_275 NTG F CR
SA023326B_285 NTG F CR
SA023327B_265 NTG F CR
SA023328B_255 NTG F CR
SA023329B_245 NTG F CR
SA023330B_555 NTG M AL
SA023331B_585 NTG M AL
SA023332B_575 NTG M AL
SA023333B_565 NTG M AL
SA023334B_595 NTG M AL
SA023335B_635 NTG M CR
SA023336B_605 NTG M CR
SA023337B_625 NTG M CR
SA023338B_645 NTG M CR
SA023339B_615 NTG M CR
SA023340BP_1Pool Pool Pool Pool
SA023341BP_2Pool Pool Pool Pool
SA023342BP_6Pool Pool Pool Pool
SA023343BP_5Pool Pool Pool Pool
SA023344BP_4Pool Pool Pool Pool
SA023345BP_3Pool Pool Pool Pool
Showing results 1 to 78 of 78


Collection ID:CO000477
Collection Summary:Brain (without hippocampus and entorhinal cortex)
Sample Type:Brain


Treatment ID:TR000497
Treatment Summary:Calorie restricted (CR) and ad libitum (AL) diets

Sample Preparation:

Sampleprep ID:SP000490
Sampleprep Summary:Brain tissue samples were homogenized in 50:50 Acetonitrile:Water , and the final concentration of all of the extracts was 1 mL/mg. Of the 71 samples, 48 had sufficient volume for study samples and for an analytical quality control (QC) total pool sample. This total pool was generated by transferring a 50 µL of each sample and aliquoting into six replicates, which were processed identically to the study samples. A 250 µL aliquot of each sample homogenate was lyophilized and then reconstituted with 250 µL of Phosphate buffer with Chenomx ISTD and 6mM Imidazole. Samples were vortexed and centrifuged before transferring 225 µL of each sample into pre-labeled 3mm NMR tubes for data acquisition on a 700 MHz spectrometer.


Analysis ID:AN000723
Analysis Type:NMR
Num Factors:17


NMR ID:NM000080
Analysis ID:AN000723
Instrument Name:Bruker Avance III
Instrument Type:FT-NMR
NMR Experiment Type:1D 1H
Spectrometer Frequency:700 MHz