Summary of Study ST000291
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000233. The data can be accessed directly via it's Project DOI: 10.21228/M8J590 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST000291 |
Study Title | LC-MS Based Approaches to Investigate Metabolomic Differences in the Urine of Young Women after Drinking Cranberry Juice or Apple Juice |
Study Type | drug dosage |
Study Summary | Eighteen healthy female college students between 21-29 years old with a normal BMI of 18.5-25 were recruited. Each subject was provided with a list of foods that contained significant amount of procyanidins, such as cranberries, apples, grapes, blueberries, chocolate and plums. They were advised to avoid these foods during the 1-6th day and the rest of the study. On the morning of the 7th day, a first-morning baseline urine sample and blood sample were collected from all human subjects after overnight fasting. Participants were then randomly allocated into two groups (n=9) to consume cranberry juice or apple juice. Six bottles (250 ml/bottle) of juice were given to participants to drink in the morning and evening of the 7th, 8th, and 9th day. On the morning of 10th day, all subjects returned to the clinical unit to provide a first-morning urine sample after overnight fasting. The blood sample was also collected from participants 30 min later after they drank another bottle of juice in the morning. After two-weeks of wash out period, participants switched to the alternative regimen and repeated the protocol. One human subject was dropped off this study because she missed part of her appointments. Another two human subjects were removed from urine metabolomics analyses because they failed to provide required urine samples after juice drinking.The present study aimed to investigate overall metabolic changes caused by procyanidins concentrates from cranberries and apples using a global LCMS based metabolomics approach. All plasma and urine samples were stored at -80oC until analysis. |
Institute | University of Florida |
Department | SECIM |
Laboratory | Gu |
Last Name | Liu |
First Name | Haiyan |
Address | -- |
haiyan66@ufl.edu | |
Phone | 352-392-1991x210 |
Submit Date | 2014-11-17 |
Num Groups | 3 |
Total Subjects | 45 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzXML |
Analysis Type Detail | LC-MS |
Release Date | 2016-12-22 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
Project ID: | PR000233 |
Project DOI: | doi: 10.21228/M8J590 |
Project Title: | LC-MS Based Approaches to Investigate Metabolomic Differences in the Urine and Plasma of Young Women after Drinking Cranberry Juice or Apple Juice |
Project Summary: | The present study aimed to investigate overall metabolic changes caused by cranberry juice or apple juice consumption using a global LC-MS based metabolomics approach. |
Institute: | University of Florida |
Department: | Food Science and Nutrition |
Laboratory: | Gu |
Last Name: | Liu |
First Name: | Haiyan |
Address: | -- |
Email: | haiyan66@ufl.edu |
Phone: | 352-392-1991x210 |
Subject:
Subject ID: | SU000311 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Subject Comments: | Gu_subjects_human urine.txt |
Species Group: | Mammals |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
mb_sample_id | local_sample_id | Treatment |
---|---|---|
SA013217 | b15 | Baseline urine |
SA013218 | b13 | Baseline urine |
SA013219 | b16 | Baseline urine |
SA013220 | b17 | Baseline urine |
SA013221 | b1 | Baseline urine |
SA013222 | b2 | Baseline urine |
SA013223 | b12 | Baseline urine |
SA013224 | b14 | Baseline urine |
SA013225 | b7 | Baseline urine |
SA013226 | b11 | Baseline urine |
SA013227 | b4 | Baseline urine |
SA013228 | b8 | Baseline urine |
SA013229 | b6 | Baseline urine |
SA013230 | b9 | Baseline urine |
SA013231 | b10 | Baseline urine |
SA013232 | a8 | Urine after drinking apple juice |
SA013233 | a9 | Urine after drinking apple juice |
SA013234 | a7 | Urine after drinking apple juice |
SA013235 | a6 | Urine after drinking apple juice |
SA013236 | a10 | Urine after drinking apple juice |
SA013237 | a1 | Urine after drinking apple juice |
SA013238 | a4 | Urine after drinking apple juice |
SA013239 | a2 | Urine after drinking apple juice |
SA013240 | a16 | Urine after drinking apple juice |
SA013241 | a17 | Urine after drinking apple juice |
SA013242 | a15 | Urine after drinking apple juice |
SA013243 | a14 | Urine after drinking apple juice |
SA013244 | a12 | Urine after drinking apple juice |
SA013245 | a13 | Urine after drinking apple juice |
SA013246 | a11 | Urine after drinking apple juice |
SA013247 | c10 | Urine after drinking cranberry juice |
SA013248 | c7 | Urine after drinking cranberry juice |
SA013249 | c8 | Urine after drinking cranberry juice |
SA013250 | c6 | Urine after drinking cranberry juice |
SA013251 | c4 | Urine after drinking cranberry juice |
SA013252 | c1 | Urine after drinking cranberry juice |
SA013253 | c2 | Urine after drinking cranberry juice |
SA013254 | c9 | Urine after drinking cranberry juice |
SA013255 | c11 | Urine after drinking cranberry juice |
SA013256 | c15 | Urine after drinking cranberry juice |
SA013257 | c16 | Urine after drinking cranberry juice |
SA013258 | c14 | Urine after drinking cranberry juice |
SA013259 | c13 | Urine after drinking cranberry juice |
SA013260 | c12 | Urine after drinking cranberry juice |
SA013261 | c17 | Urine after drinking cranberry juice |
Showing results 1 to 45 of 45 |
Collection:
Collection ID: | CO000305 |
Collection Summary: | n/a |
Collection Protocol Filename: | Gu_collection_human urine.txt |
Sample Type: | Urine |
Treatment:
Treatment ID: | TR000325 |
Treatment Summary: | n/a |
Treatment Protocol Filename: | Gu_treatment_human urine.txt |
Sample Preparation:
Sampleprep ID: | SP000319 |
Sampleprep Summary: | n/a |
Sampleprep Protocol Filename: | Metabolomics_LCMSProtocol_urine.pdf |
Combined analysis:
Analysis ID | AN000464 | AN000465 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Scientific-Dionex Ultimate 3000 | Thermo Scientific-Dionex Ultimate 3000 |
Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | Peak area | Peak area |
Chromatography:
Chromatography ID: | CH000330 |
Methods Filename: | Metabolomics_LCMSProtocol_urine.pdf |
Instrument Name: | Thermo Scientific-Dionex Ultimate 3000 |
Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
Flow Rate: | 0.350mL/min-0.600mL/min |
Internal Standard: | Appendix A - Internal Standard Prep GLCMS.pdf |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000404 |
Analysis ID: | AN000464 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | POSITIVE |
Analysis Protocol File: | Metabolomics_LCMSProtocol_urine.pdf |
MS ID: | MS000405 |
Analysis ID: | AN000465 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | NEGATIVE |
Analysis Protocol File: | Metabolomics_LCMSProtocol_urine.pdf |