Summary of Study ST003090

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001919. The data can be accessed directly via it's Project DOI: 10.21228/M8P13K This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST003090
Study Title	Analysis of lipid profiles of N2A-Pz1-KO cells expressing tdTOMATO-vector (mock), TMEM120A, or TMEM120B
Study Type	Lipidomic
Study Summary	N2A-Pz1-KO cells expressing tdTOMATO-vector (mock), TMEM120A, or TMEM120B were used to detect differences in levels of lipids. TMEM120A is a protein without a clear function that we previously identified to inhibit mechanically activated PIEZO2 channels. TMEM120A has structural similarity a lipid modifying enzyme (ELOVL7), and has been proposed to be necessary for adipocyte maturation & triglyceride production. We performed LC-MS/MS to determine whether TMEM120A can modify the lipid content of cells where it is expressed and utilize that data to assess whether those lipids can inhibit PIEZO2 channels. This study revealed that TMEM120A expressing cells do have larger levels of phosphatidic acid and lysophosphatidic acid, and subsequent functional studies revealed that these lipids do inhibit PIEZO2.
Institute	Rutgers University
Department	Pharmacology, Physiology and Neuroscience; Metabolomics Shared Resource
Laboratory	Tibor Rohacs; Xiaoyang Su
Last Name	Rohacs
First Name	Tibor
Address	183 South Orange Ave, Newark, NJ, 07103
Email	rohacsti@njms.rutgers.edu
Phone	973-972-4464
Submit Date	2024-02-01
Num Groups	3
Total Subjects	9
Raw Data Available	Yes
Raw Data File Type(s)	mzXML
Analysis Type Detail	LC-MS
Release Date	2024-07-16
Release Version	1

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Treatment:

Treatment ID:	TR003214
Treatment Summary:	N2A-Pz1-KO cells were transfected using Polyethylenimine Max with 1500 ng of the vectors for the expression of tdTOMATO (mock), TMEM120A, or TMEM120B. 48 hours after transfection, the cells were treated with trypsin for 3 minutes at 37°C for collection, and counted via hemocytometer. One million cells per group were centrifuged to remove media and trypsin, and then washed with ice-cold PBS before the lipid extraction was performed.

Treatment ID:

TR003214

Treatment Summary:

N2A-Pz1-KO cells were transfected using Polyethylenimine Max with 1500 ng of the vectors for the expression of tdTOMATO (mock), TMEM120A, or TMEM120B. 48 hours after transfection, the cells were treated with trypsin for 3 minutes at 37°C for collection, and counted via hemocytometer. One million cells per group were centrifuged to remove media and trypsin, and then washed with ice-cold PBS before the lipid extraction was performed.