Summary of Study ST002270

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001452. The data can be accessed directly via it's Project DOI: 10.21228/M81Q5B This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002270
Study TitleXenopus tropicalis glycolysis and PPP inhibition
Study SummaryStage 41 tadpoles were injected with 4 nmol of the glycolysis inhibitor, 2-deoxyglucose (2DG), or a tracer control to evaluate consequences of inhibition on metabolites 24 hours after treatment began. Similarly, tadpoles were incubated in DMSO control or 1 of 2 G6PD inhibitors (Dehydroepiandrosterone and g6pdi), to similarly assess the consequences of inhibiting the pentose phosphate pathway. We find that inhibition of glucose metabolism with 2DG results in a decrease in downstream glycolytic intermediates, confirming a reduction in activity of this pathway. G6PD inhibition was not as clear as changes were less consistent across treatments and downstream metabolites did not behave in a coordinated way, though impacts on other metabolic processes by these inhibitors may be fruitful for exploring how they perturb metabolism in the tadpoles.
University of Washington
Last NamePatel
First NameJeet
Address1705 NE Pacific St., HSB J-Wing, J405, Seattle, Washington, 98195, USA
Submit Date2022-08-03
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2022-10-25
Release Version1
Jeet Patel Jeet Patel application/zip

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Treatment ID:TR002368
Treatment Summary:Dihydroepiandrosterone (BioVision, 2172) was resuspended to a 1M stock in DMSO and g6pdi (Cayman Chemical Co., 31484) to a 10mM stock in DMSO. Tadples were reared with 0.1% DMSO, 25µM DHEA or 10µM g6pdi diluted in 1/9x MR until collection after a 24 treatment. For 2DG injections, wild type tadpoles at stage 41 were anesthetized with MS-222 and moved from culture dish to a thickly coasted agarose dish in one drop of media. Excess media was removed. Using a microinjector, a pulled needle containing vMO and labeled dextran tracer was inserted into the ventral tail vein and 2×2 nL of 1M 2DG (Sigma, D8375) were injected. Controls for 2DG experiments were injected with equal volumes of tracer. Embryos were returned to fresh media and screened for tracer fluorescence in the bloodstream. Injected animals were kept in 1/9x MR until collection after a 24 treatment.