Summary of Study ST001414

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000970. The data can be accessed directly via it's Project DOI: 10.21228/M89Q4F This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001414
Study TitlePseudomonas aeruginosa cystic fibrosis clinical isolates volatile metabolomics
Study TypeUntargeted metabolomics
Study SummaryUntargeted metabolomics analysis of in vitro headspace volatiles from 81 Pseudomonas aeruginosa bacterial isolates from individuals with cystic fibrosis. Headspace volatiles were collected using solid-phase microextraction (SPME) (in triplicate) and comprehensive two-dimensional gas chromatography and time-of-flight mass spectrometry (GCxGC-TOFMS). 15 replicates of un-inoculated media were prepared and analyzed in parallel, for a total of 258 samples.
Institute
Arizona State University
DepartmentSchool of Life Sciences
LaboratoryBean Laboratory
Last NameDavis
First NameTrenton
Address427 E Tyler Mall, LSE 317, Tempe, AZ, 85287
EmailTrenton.J.Davis@asu.edu
Phone5738235718
Submit Date2020-05-31
Raw Data AvailableYes
Raw Data File Type(s)smp
Analysis Type DetailGC-MS
Release Date2020-09-22
Release Version1
Trenton Davis Trenton Davis
https://dx.doi.org/10.21228/M89Q4F
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Treatment:

Treatment ID:TR001503
Treatment Summary:Liquid cultures of lysogeny broth (Lennox) were inoculated with P. aeruginosa from pure glycerol stocks and incubated aerobically at 37 degrees Celsius for 24 hours in triplicate. Cultures were centrifuged through a 0.2 ┬Ám filter to remove cells, and filtrates were transferred to GC headspace vials.
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