Summary of Study ST000257

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000209. The data can be accessed directly via it's Project DOI: 10.21228/M8CP4Q This work is supported by NIH grant, U2C- DK119886.

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Study IDST000257
Study TitleNIH WCMC Pilot & Feasibility Project: “Metabolite changes associated with weight loss”
Study TypeFeeding study
Study SummaryTargeted metabolomic analyses of oxylipins, endocannabinoids, and ceramides were performed on 18 mouse liver, adipose, hypothalamus, plasma, and muscle samples collected from mice euthanized at 18 weeks following consumption of three diet regimens that induced lean, obese, and weight loss phenotypes. Samples were analyzed by UPLC-MS/MS using a Waters Acquity UPLC and detected on an API 4000 QTrap (AB Sciex, Framingham, MA, USA) by multiple reaction monitoring (MRM) after negative mode electrospray ionization.
Institute
University of California, Davis
DepartmentNutrition
LaboratoryNewman Lab
Last NameNewman
First NameJohn
Address430 W. Health Sciences Dr., Davis, CA 95616
Emailjohn.newman@ars.usda.gov
Phone+1-530-752-1009
Submit Date2015-01-16
Num Groups3
Total Subjects18
Raw Data AvailableYes
Raw Data File Type(s)mzML
Uploaded File Size128 MB
Analysis Type DetailLC-MS
Release Date2015-09-24
Release Version1
John Newman John Newman
https://dx.doi.org/10.21228/M8CP4Q
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Subject:

Subject ID:SU000277
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Age Or Age Range:30 weeks
Gender:Female
Animal Housing:Singly housed
Animal Feed:High fat diet (D12492, Research Diets, New Brunswick, NJ), Low fat diet (D12450, Research Diets, New Brunswick, NJ)
Species Group:Mammal
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