Summary of Study ST003661

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002271. The data can be accessed directly via it's Project DOI: 10.21228/M82V6D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST003661
Study Title	Lipidomics facilitates the discovery of diagnostic biomarkers in patients with chronic total occlusion during the perioperative period
Study Summary	Chronic total occlusion (CTO) is a subtype of cardiovascular disease associated with high mortality and an increased risk of ventricular arrhythmia. This study aimed to investigate lipidomic changes in CTO patients undergoing percutaneous coronary intervention (PCI) using a tandem-lipidomic strategy. We first applied a global lipidomic approach to identify the serum lipidomes of CTO-PCI patients during the perioperative period, successfully separating and identifying over 1,500 lipids. Based on these results, a Multiple Reaction Monitoring (MRM) quantification method was developed and employed for targeted lipidomic analysis. Using a high-throughput MRM tandem liquid chromatography-mass spectrometry approach, 613 lipids were successfully quantified in CTO-PCI patients and control donors. PA 18:2/11:0 emerged as a potential biomarker for distinguishing CTO patients from those suspected of having the condition. Notably, patients with different prognostic outcomes exhibited significantly distinct serum lipidomes in both pre- and post-CTO-PCI samples. This finding suggests that lipidomic data hold significant potential not only for monitoring postoperative prognosis but also for predicting surgical outcomes
Institute	Zhongshan Hospital Fudan University
Last Name	Wang
First Name	Zhenxin
Address	136 Yi Xue Yuan Road
Email	wang.zhenxin@zs-hospital.sh.cn
Phone	+8618817976583
Submit Date	2024-12-04
Num Groups	4
Total Subjects	63
Raw Data Available	Yes
Raw Data File Type(s)	wiff
Analysis Type Detail	LC-MS
Release Date	2025-01-20
Release Version	1

Select appropriate tab below to view additional metadata details:

Sample Preparation:

Sampleprep ID:	SP003801
Sampleprep Summary:	Lipid extraction. The whole blood was kept quiescent at room temperature for 1 hr and centrifuged at 850 × g for 10 mins at 4℃ to separate the serum. A modified Bligh & Dyer method was u sed to extract lipids from the serum samples, as previously described 33, 34. In brief, 200 μL serum was subjected to a quick freeze-and-thaw five times using liquid nitrogen. 5 mL of a monophasic mixture of methanol: chloroform: formic acid (10:10:1) was then added. The mixture was shaken vigorously and incubated at -20℃ overnight. After 2.2 mL Hajra’s solution (0.2M H3PO4, 1M KCl) was added to each tube and mixed by vigorous shaking, the samples were centrifuged at 1,500 × g for 5 min. The lower CHCl3 phase was withdrawn to a new glass tube and the upper phase was re-extracted with 0.5 mL CHCl3. After the combination of CHCl3 phase, each sample was evaporated to 100 µL under nitrogen gas. A modified neutral lipid extraction method was used to extract lipids from the serum samples 35. An internal standard cocktail (Avanti Lipids Polar, Inc., USA) containing phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylserine (PS), phosphatidylglycerol (PG), phosphatidylinositol (PI), phosphatidic acid (PA), lysophosphatidylcholine (LPC), lysophosphatidylethanolamine (LPE), cholesterol ester (CE), triacylglycerol (TAG), diacylglycerol (DAG), sphingomyelin (SM) and ceramide (CER) was added to each sample at an amount of 10 μL per 200 μL of serum during the extraction. Concentration of each lipid class optimized for serum analysis.

Sampleprep ID:

SP003801

Sampleprep Summary:

Lipid extraction. The whole blood was kept quiescent at room temperature for 1 hr and centrifuged at 850 × g for 10 mins at 4℃ to separate the serum. A modified Bligh & Dyer method was u sed to extract lipids from the serum samples, as previously described 33, 34. In brief, 200 μL serum was subjected to a quick freeze-and-thaw five times using liquid nitrogen. 5 mL of a monophasic mixture of methanol: chloroform: formic acid (10:10:1) was then added. The mixture was shaken vigorously and incubated at -20℃ overnight. After 2.2 mL Hajra’s solution (0.2M H3PO4, 1M KCl) was added to each tube and mixed by vigorous shaking, the samples were centrifuged at 1,500 × g for 5 min. The lower CHCl3 phase was withdrawn to a new glass tube and the upper phase was re-extracted with 0.5 mL CHCl3. After the combination of CHCl3 phase, each sample was evaporated to 100 µL under nitrogen gas. A modified neutral lipid extraction method was used to extract lipids from the serum samples 35. An internal standard cocktail (Avanti Lipids Polar, Inc., USA) containing phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylserine (PS), phosphatidylglycerol (PG), phosphatidylinositol (PI), phosphatidic acid (PA), lysophosphatidylcholine (LPC), lysophosphatidylethanolamine (LPE), cholesterol ester (CE), triacylglycerol (TAG), diacylglycerol (DAG), sphingomyelin (SM) and ceramide (CER) was added to each sample at an amount of 10 μL per 200 μL of serum during the extraction. Concentration of each lipid class optimized for serum analysis.