Summary of Study ST003220

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002008. The data can be accessed directly via it's Project DOI: 10.21228/M8281N This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST003220
Study Title	Obesity, sex, and depot drive distinct lipid profiles in murine white adipose tissue
Study Summary	To investigate how the lipid composition of the adipose tissue changes with obesity, sex, and depot, we performed comprehensive untargeted lipidomics on the whole adipose tissue of the inguinal and perigonadal adipose depot from lean and obese, male and female mice. The screen allowed us to identify lipid signatures sufficient to differentiate different adipose tissue samples. Such that, increased levels of OxTG and CL, increase in DG and Cer, and increase in HexCer were all able to differentiate adipose tissue based on obesity, sex, or depot, respectively.
Institute	University of Utah
Last Name	Hilgendorf
First Name	Keren
Address	EEJMRB 5520
Email	keren.hilgendorf@biochem.utah.edu
Phone	(801) 587-1071
Submit Date	2023-10-25
Raw Data Available	Yes
Raw Data File Type(s)	mzML
Analysis Type Detail	LC-MS
Release Date	2024-06-12
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP003346
Sampleprep Summary:	Extraction of lipids was carried out using a biphasic solvent system of cold methanol (MeOH), methyl tert-butyl ether (MTBE), and water as described by Matyash et al. (J Lipid Res 49(5) (2008) 1137-1146) with some modifications. To begin, tissues were homogenized in PBS so that each samples final concentration was equal to 159.6 mg/mL. From each sample, 188 µL was aliquoted into a separate, labelled microcentrifuge tube (polypropylene 1.7 mL, VWR, USA), equal to 30 mg of sample. Tissue lipids were extracted in a solution of 750 µL MTBE and 225 µL MeOH with internal standards. Samples were sonicated for 2 minutes then rested on ice for 1 hr with occasional vortexing. Samples were then centrifuged at 15,000 x g for 10 minutes at 4 °C, and the upper phases were collected. Another aliquot of 1 mL MTBE/MeOH/water (10/3/2.5, v/v/v) was added to the bottom aqueous layer followed by a brief vortex. Samples were then centrifuged at 15,000 x g for 10 minutes at 4 °C, the upper phases were combined and evaporated to dryness under speedvac. After dry down, there was approximately 200 µL of undried material left over. This prompted re-extraction. To each sample was added 1 mL of MTBE/MeOH (7.5/1, v/v), followed by a brief vortex, centrifugation at 15,000 x g for 10 minutes at 4 °C, and collection of upper phases. Another addition of 1 mL MTBE/MeOH (7.5/1, v/v) was added to the bottom aqueous layer followed by a brief vortex, centrifugation at 15,000 x g for 10 minutes at 4 °C, and the combination of upper phases which were evaporated to dryness under speedvac. Lipid extracts were reconstituted in IPA/ACN/water (4:1:1, v/v/v). Concurrently, a process blank sample and pooled quality control (QC) samples were prepared.

Sampleprep ID:

SP003346

Sampleprep Summary:

Extraction of lipids was carried out using a biphasic solvent system of cold methanol (MeOH), methyl tert-butyl ether (MTBE), and water as described by Matyash et al. (J Lipid Res 49(5) (2008) 1137-1146) with some modifications. To begin, tissues were homogenized in PBS so that each samples final concentration was equal to 159.6 mg/mL. From each sample, 188 µL was aliquoted into a separate, labelled microcentrifuge tube (polypropylene 1.7 mL, VWR, USA), equal to 30 mg of sample. Tissue lipids were extracted in a solution of 750 µL MTBE and 225 µL MeOH with internal standards. Samples were sonicated for 2 minutes then rested on ice for 1 hr with occasional vortexing. Samples were then centrifuged at 15,000 x g for 10 minutes at 4 °C, and the upper phases were collected. Another aliquot of 1 mL MTBE/MeOH/water (10/3/2.5, v/v/v) was added to the bottom aqueous layer followed by a brief vortex. Samples were then centrifuged at 15,000 x g for 10 minutes at 4 °C, the upper phases were combined and evaporated to dryness under speedvac. After dry down, there was approximately 200 µL of undried material left over. This prompted re-extraction. To each sample was added 1 mL of MTBE/MeOH (7.5/1, v/v), followed by a brief vortex, centrifugation at 15,000 x g for 10 minutes at 4 °C, and collection of upper phases. Another addition of 1 mL MTBE/MeOH (7.5/1, v/v) was added to the bottom aqueous layer followed by a brief vortex, centrifugation at 15,000 x g for 10 minutes at 4 °C, and the combination of upper phases which were evaporated to dryness under speedvac. Lipid extracts were reconstituted in IPA/ACN/water (4:1:1, v/v/v). Concurrently, a process blank sample and pooled quality control (QC) samples were prepared.