Summary of Study ST002859
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001788. The data can be accessed directly via it's Project DOI: 10.21228/M8KX5C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002859 |
Study Title | Glucose tracing in vehicle- and Enzalutamide-treated 16D prostate cancer cells |
Study Summary | In this study, we sought to determine how Enzalutamide treatment alters metabolism in 16D prostate cancer cells. |
Institute | University of California, Los Angeles |
Laboratory | Goldstein Lab |
Last Name | Giafaglione |
First Name | Jenna |
Address | 610 Charles E Young Dr S, Los Angeles, CA, 90024, USA |
jgiafaglione@g.ucla.edu | |
Phone | 408-728-3065 |
Submit Date | 2023-09-12 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | GC-MS |
Release Date | 2023-09-15 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP002977 |
Sampleprep Summary: | Cell plates were placed on ice and quickly washed with ice-cold 0.9% (w/v) NaCl. Cells were immediately treated with 500 μL of ice-cold MeOH and 200 μL water containing 1 μg of the internal standard norvaline. Cells were then scraped and placed in 1.5 mL centrifuge tubes kept on ice. Next, 500 μL of chloroform was added, after which samples were vortexed for 1 minute and then spun at 10,000g for 5 minutes at 4C. The aqueous layer was transferred to a GC/MS sample vial and dried overnight using a refrigerated CentriVap. |