Summary of Study ST002804
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001751. The data can be accessed directly via it's Project DOI: 10.21228/M8CH9W This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002804 |
| Study Title | Metabolic Adaptations of Microbacterium sediminis YLB-01 for Survival in the Challenging High-Pressure of the Deep Sea |
| Study Summary | By using NMR-based metabolomic analysis, we investigated the metabolic adaptations of Microbacterium sediminis YLB-01 in response to high-pressure conditions. We recorded the 600 MHz 1D 1H-NMR spectra on aqueous extracts from YLB-01 cells, and then assigned resonances of 31 metabolites. The distinct metabolic separation between the HPLT and NPLT groups highlighted the significant effect of high-pressure treatment on the metabolism of YLB-01 cells. |
| Institute | Xiamen University |
| Last Name | Qiu |
| First Name | Xu |
| Address | No. 422, Siming South Road, Xiamen, Fujian, China. |
| qiuxu@stu.xmu.edu.cn | |
| Phone | 13161342734 |
| Submit Date | 2023-06-18 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | fid |
| Analysis Type Detail | NMR |
| Release Date | 2023-08-22 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP002917 |
| Sampleprep Summary: | After culturing the YLB-01 cells, each 100 mL of the culture was transferred into a 250-mL centrifuge bottle and centrifuged at 4°C (6000 g, 5 min). The supernatant was carefully decanted, and the cell pellets were rapidly cooled to -40°C using 100 mL of a buffer composed of a 3:2 methanol/water mixture containing 0.85% (wt/vol) NaCl. The mixture was centrifuged again at 4°C (6000 g, 5 min). The cell pellets were washed three times with 5 mL of cold phosphate-buffered saline (PBS) and centrifuged at 4°C (6000 g, 5 min) after each wash. Finally, the cell pellets were stored at -80°C until further use. Initially, 600 μL of a cold extraction buffer consisting of a 1:1 mixture of distilled water and acetonitrile was added to homogenize the samples. The mixtures were then sonicated on wet ice for 180 cycles, with each cycle consisting of 2 seconds of ultrasound followed by a 3-second pause. After centrifugation at 4°C (12000 g, 10 min), the supernatants were collected and lyophilized, resulting in an extract powder that was stored at -80°C for further analysis. |
| Processing Storage Conditions: | Described in summary |
| Extract Storage: | Described in summary |
