Summary of Study ST002802

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001749. The data can be accessed directly via it's Project DOI: 10.21228/M8N14N This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002802
Study TitleNontargeted Serum Metabolomic Profiling of FXR-null and Wild-type Mice
Study SummaryThis study analyzed the serum metabolome of 10 FXR-knockout and 10 wild-type mice by RPLC-HRMS.
Institute
University of North Carolina at Chapel Hill
DepartmentDepartment of Environmental Sciences and Engineering
LaboratoryKun Lu
Last NameHsiao
First NameYun-Chung
Address135 Dauer Dr. MHRC1104
Emailychsiao@live.unc.edu
Phone9842159592
Submit Date2023-07-31
Num Groups2
Total Subjects20
Num Males20
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2023-08-15
Release Version1
Yun-Chung Hsiao Yun-Chung Hsiao
https://dx.doi.org/10.21228/M8N14N
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP002915
Sampleprep Summary:Serum samples from each mouse were thawed and 20 μL was consumed to extract metabolites for each mice. Metabolite extraction was facilitated by adding 180 μL methanol containing stable isotope-labeled chemicals ([D5]-glutamine, [D2]-𝛾-aminobutyric acid, [D3]-tryptophan, [D2]-indole-3-propionic acid, [D2]-indole-3-acetic acid, [D13]-acetylcholine in 500 nM; [D4]-serotonin in 250 nM; and [D4]-kynurenic acid in 50 nM), and incubated under -20°C for 1 hr, followed by centrifugation under 15,000 ×g, 4°C for 10 minutes to collect the supernatant (150 μL). The supernatant was dried by SpeedVac® and reconstituted with 100 μL 2% acetonitrile in water.
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