Summary of Study ST002774

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001731. The data can be accessed directly via it's Project DOI: 10.21228/M8ZD8R This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002774
Study Title	Metabolomics studies on L4-5 Dorsal Root Ganglia of Ctrl and cKO mice
Study Summary	Metabolomics studies on Dorsal Root Ganglia (DRGs) (Ctrl and cKO).The specific knockout of PP2Cm in the DRG was achieved by intracellular injection of AAV9-Pirt-Cre (1.0×1013 vg/ml) or AAV9-Pirt (1.0×1013 vg/ml)) as control. Mice were used for experiments at 4 weeks after the injection.
Institute	West China Hospital of Sichuan University
Last Name	Li
First Name	Tao
Address	No. 37 Guoxue Road, Wuhou District, Chendu 610041, Sichuan, China
Email	563234879@qq.com
Phone	+8628-85164154
Submit Date	2023-07-07
Raw Data Available	Yes
Raw Data File Type(s)	mzML
Analysis Type Detail	LC-MS
Release Date	2023-09-14
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP002887
Sampleprep Summary:	Fresh tissue samples were frozen in liquid nitrogen. After thawing on ice, 10 mg tissue was homogenized in -80°C precooled labeled-solution (MeOH: H2O=4:1, v/v) for 30 s, 4 cycles, and placed at -80°C for 30 minutes. In ice water bath, ultrasonic wave was used for 10 min and centrifuged at 14000 rpm. The precipitated protein was dissolved in no labeled-liquid (MeOH: H2O=4:1), vortexed and centrifuged. After derivatization with N-methyl-N-(trimethylsilyl)tri-fluoroacetamide for 30 min, the supernatant was detected by GC-MS. Metabolites were identified by means of KEGG Metabolic pathways.

Sampleprep ID:

SP002887

Sampleprep Summary:

Fresh tissue samples were frozen in liquid nitrogen. After thawing on ice, 10 mg tissue was homogenized in -80°C precooled labeled-solution (MeOH: H2O=4:1, v/v) for 30 s, 4 cycles, and placed at -80°C for 30 minutes. In ice water bath, ultrasonic wave was used for 10 min and centrifuged at 14000 rpm. The precipitated protein was dissolved in no labeled-liquid (MeOH: H2O=4:1), vortexed and centrifuged. After derivatization with N-methyl-N-(trimethylsilyl)tri-fluoroacetamide for 30 min, the supernatant was detected by GC-MS. Metabolites were identified by means of KEGG Metabolic pathways.