Summary of Study ST002741

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001706. The data can be accessed directly via it's Project DOI: 10.21228/M8642W This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002741
Study Title	Integration of Meta-Multi-Omics Data Using Probabilistic Graphs and External Knowledge
Study Summary	Multi-omics has the promise to provide a detailed molecular picture for biological systems. Although obtaining multi-omics data is relatively easy, methods that analyze such data have been lagging. In this paper, we present an algorithm that uses probabilistic graph representations and external knowledge to perform optimum structure learning and deduce a multifarious interaction network for multi-omics data from a bacterial community. Kefir grain, a microbial community that ferments milk and creates kefir, represents a self-renewing, stable, natural microbial community. Kefir has been shown to associate with a wide range of health benefits. We obtained a controlled bacterial community using the two most abundant and well-studied species in kefir grains: Lentilactobacillus kefiri and Lactobacillus kefiranofaciens. We applied growth temperatures of 30°C and 37°C, and obtained transcriptomic, metabolomic, and proteomic data for the same 20 samples (10 samples per temperature). We obtained a multi-omics interaction network, which generated insights that would not have been possible with single-omics analysis. We identified interactions among transcripts, proteins, and metabolites suggesting active toxin/antitoxin systems. We also observed multifarious interactions that involved the shikimate pathway. These observations helped explain bacterial adaptation to different stress conditions, co-aggregation, and increased activation of L. kefiranofaciens at 37°C.
Institute	University of Nebraska-Lincoln
Last Name	Alvarez
First Name	Sophie
Address	1901 Vine St
Email	salvarez@unl.edu
Phone	4024724575
Submit Date	2023-06-19
Raw Data Available	Yes
Raw Data File Type(s)	abf, d
Analysis Type Detail	GC-MS
Release Date	2023-08-10
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP002924
Sampleprep Summary:	Cell pellets were washed 3 times with cold PBS to remove any cell media left after collection. The cell pellets were then extracted using cold 100% methanol and spiked with 40 μL of 10 pinitol (internal standard). A quality control (QC) sample was prepared by mixing the same amount of each sample into one. The supernatants were then dried down using a speed-vac and then resuspended in 20 mg/mL methoxyamine hydrochloride reagent prepared in pure pyridine and incubated for 2 hr at 37 °C on a platform shaker at 1000 rpm. Next, for derivatization, the MSTFA +1% TMCS deri-vatization (ThermoFisher) was added to each sample, incubated for 30 min at 37°C on a platform shaker at 1000 rpm followed by a centrifugation for 10 min at 16,000 g prior to transferring the mixture to GC vials for injection into GC-MS.

Sampleprep ID:

SP002924

Sampleprep Summary:

Cell pellets were washed 3 times with cold PBS to remove any cell media left after collection. The cell pellets were then extracted using cold 100% methanol and spiked with 40 μL of 10 pinitol (internal standard). A quality control (QC) sample was prepared by mixing the same amount of each sample into one. The supernatants were then dried down using a speed-vac and then resuspended in 20 mg/mL methoxyamine hydrochloride reagent prepared in pure pyridine and incubated for 2 hr at 37 °C on a platform shaker at 1000 rpm. Next, for derivatization, the MSTFA +1% TMCS deri-vatization (ThermoFisher) was added to each sample, incubated for 30 min at 37°C on a platform shaker at 1000 rpm followed by a centrifugation for 10 min at 16,000 g prior to transferring the mixture to GC vials for injection into GC-MS.