Summary of Study ST002569

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001656. The data can be accessed directly via it's Project DOI: 10.21228/M8ND9B This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002569
Study TitleLipidomic analysis in very-long chain acyl-CoA dehydrogenase null mice
Study SummaryCompared to their littermate counterparts, HFD/fasted VLCAD-/- mouse hearts displayed higher VLC-acylcarnitines accumulation, higher levels of arachidonic acid (AA) and lower docosahexaenoic acid (DHA) contents in glycerophospholipids (GPLs).
Montreal Heart Institute
Last NameForest
First NameAnik
Address5000 Bélanger Street
Phone5143763330 ext 2133
Submit Date2023-04-11
Analysis Type DetailLC-MS
Release Date2023-09-06
Release Version1
Anik Forest Anik Forest application/zip

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Sample Preparation:

Sampleprep ID:SP002676
Sampleprep Summary:Total lipids were extracted from 40mg of pulverized hearts under liquid nitrogen using in methyl tert-butyl ether (MTBE) and ethyl acetate after spiking with the following internal standards lysophosphatidylcholine (LPC) 13:0, phosphatidylcholine (PC) 19:0/19:0, PC14:0/14:0, phosphatidylserine (PS) 12:0/12:0, phosphatidylglycerol (PG) 15:0/15:0 and phosphatidylethanolamine (PE) 17:0/17:0). Supernatants were evaporated using a Speed Vacuum concentrator overnight, dissolved in methanol/chloroform (2:1) and aliquots (50 µL) were stored at -80ºC until use.
Sampleprep Protocol Filename:Sample preparation protocol of untargeted lipidomic analysis
Processing Storage Conditions:-80℃