Summary of Study ST002335

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001498. The data can be accessed directly via it's Project DOI: 10.21228/M83D9C This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002335
Study Title	Pathogenic Auxilin mutations affect lipids that are critical to Synaptojanin function
Study Summary	Mass spectrometry was performed on heads of 15DO flies homogenized in 100 µl D-PBS (Dulbecco’s phosphate-buffered saline without Mg2+ and Ca2+) by Lipotype. Fifteen fly heads were pooled from three independent crosses for each analysis, and mass spectrometry was performed on n=7 for wild-type, n≥4 analyses for dAuxWT/WT, dAuxRG/RG, dAuxWT/F956x and dAuxRG/F956x and n=3 for dAuxWT/F956x and dAuxRG/F956x overexpressing Synj.
Institute	VIB-KU Leuven
Last Name	Jacquemyn
First Name	Julie
Address	Medical Sciences Building 7-25, Edmonton, Alberta, T6H 0L2, Canada
Email	jacquemynjulie@ualberta.ca
Phone	+1 (587) 3409325
Submit Date	2022-10-27
Analysis Type Detail	LC-MS
Release Date	2022-11-21
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP002429
Sampleprep Summary:	Lipids were extracted using chloroform and methanol (1). Prior to lipid extraction, samples were spiked with lipid class-specific internal standards. After drying and resuspending in an appropriate MS acquisition mixture, lipid extracts were infused directly in QExactive mass spectrometer (Thermo Fisher Scientific) with TriVersa NanoMate ion source (Advion Biosciences). Samples are analyzed in both positive and negative ion modes, with MS resolution Rm/z=200=280000 and MSMS resolution Rm/z=200=17500, in a single acquisition. Sample preparations and analysis were done by Lipotype. (1) Sampaio, J. L. et al. Membrane lipidome of an epithelial cell line. Proceedings of the National Academy of Sciences 108, 1903–1907 (2011).

Sampleprep ID:

SP002429

Sampleprep Summary:

Lipids were extracted using chloroform and methanol (1). Prior to lipid extraction, samples were spiked with lipid class-specific internal standards. After drying and resuspending in an appropriate MS acquisition mixture, lipid extracts were infused directly in QExactive mass spectrometer (Thermo Fisher Scientific) with TriVersa NanoMate ion source (Advion Biosciences). Samples are analyzed in both positive and negative ion modes, with MS resolution Rm/z=200=280000 and MSMS resolution Rm/z=200=17500, in a single acquisition. Sample preparations and analysis were done by Lipotype. (1) Sampaio, J. L. et al. Membrane lipidome of an epithelial cell line. Proceedings of the National Academy of Sciences 108, 1903–1907 (2011).