Summary of Study ST002327
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001491. The data can be accessed directly via it's Project DOI: 10.21228/M80M7R This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST002327 |
| Study Title | Effect of PARK7 gene KO on midbrain organoids |
| Study Summary | DJ1 KO was generated in BJsips iPSC and differentiated into midbrain organoids with the respective iPSC controls. The midbrain organoids were collected at day 40, 100 and 200 after differentiation. |
| Institute | Icahn School of Medicine at Mount Sinai |
| Last Name | Morrone Parfitt |
| First Name | Gustavo |
| Address | One Gustave L. Levy Place, Box 1065 |
| gustavo.parfitt@mssm.edu | |
| Phone | 212-659-5993 |
| Submit Date | 2022-09-06 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-11-18 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP002420 |
| Sampleprep Summary: | After colection,the organoids were extracted in 1 mL/well of 80:20 (v/v) methanol:water with 0.01 ng/mL Val-d8 and Phe-d8 internal extraction standards. extraction solvent was dried under nitrogen gas and metabolite samples were stored at −80 °C until analysis. |
| Sampleprep Protocol Filename: | metabolomics_methods.pdf |