Summary of Study ST002284
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001464. The data can be accessed directly via it's Project DOI: 10.21228/M8GT5D This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002284 |
| Study Title | Genetically defined human GBM organoids reveal principles of GBM development and actionable targets |
| Study Summary | Recent advances in glioblastoma (GBM) studies provide a comprehensive catalog of its genetic aberrations and cellular heterogeneity. However, a solid understanding of genotype-based analysis of cancer pathway dependency and actionable target identification is required to transform GBM treatment into a personalized era. Here, we generated a spectrum of mutant iPSCs harboring frequent GBM mutations with CRISPR/Cas9 and profiled the organoids (LEGO: Laboratory Engineered Glioblastoma Organoid) derived from these iPSCs temporally on transcriptome, methylome, metabolome, lipidome, proteome, and phospho-proteome levels. We found that LEGOs form brain tumors in vivo and recapitulate critical features of human GBM. The multi-omics analysis discovered essential milestones driven by genetic heterogeneity during GBM progressions, such as lineage alteration, methylome rewriting, and metabolome/lipidome reprogramming, in concordance with altered pathway activity and drug response. This study provides a tool and research path to realizing genome-based personalized GBM therapy using novel advanced models. |
| Institute | DKFZ |
| Department | Molecular Neurogenetics |
| Laboratory | Molecular Neurogenetics |
| Last Name | Wang |
| First Name | Changwen |
| Address | Im Neuenheimer Feld 581 |
| c.wang@dkfz-heidelberg.de | |
| Phone | +49 6221 42 3455 |
| Submit Date | 2022-09-17 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-01-26 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP002376 |
| Sampleprep Summary: | Water-soluble metabolites and lipids were extracted from the organoids and corresponding culture medium. Details are described in the Sampleprep Protocol Files (WSplusLipid.pdf) |
