Summary of Study ST002269
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001451. The data can be accessed directly via it's Project DOI: 10.21228/M85H8N This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002269 |
| Study Title | Targeted plasma metabolomics in pediatric NAFLD patients |
| Study Summary | Several adult omics studies have been conducted to understand the pathophysiology of nonalcoholic fatty liver disease (NAFLD). However, the histological features of children are different from those of adults, and the onset and progression of pediatric NAFLD are not fully understood. In this study, we aimed to evaluate the metabolome profile and metabolic pathway changes associated with pediatric NAFLD to elucidate its pathophysiology. We analyzed the metabolic profiles of healthy control, lean NAFLD, overweight control and overweight NAFLD groups of children and adolescent participants (n = 165) by assessing plasma samples, and identified 18 NAFLD-specific metabolic features and metabolic changes in lipid, glutathione-related amino acid, and branched-chain amino acid metabolism by comparing control and NAFLD group in pediatric population with overweight. Metabolome changes in the plasma of pediatric patients with NAFLD are associated with the pathophysiology of the disease and can be utilized as a less-invasive approach to diagnose the disease. |
| Institute | Seoul National University College of Medicine and Hospital |
| Department | Department of Clinical Pharmacology and Therapeutics |
| Last Name | Chae |
| First Name | Woori |
| Address | 101 Daehak-ro, Jongno-gu, Seoul 03080, Republic of Korea |
| yunus@snu.ac.kr | |
| Phone | 82-2-740-8905 |
| Submit Date | 2022-08-07 |
| Num Groups | 4 |
| Total Subjects | 165 |
| Num Males | 122 |
| Num Females | 43 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS/MS(Dir. Inf.) |
| Release Date | 2023-08-07 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP002361 |
| Sampleprep Summary: | frozen plasma samples were thawed on ice and vortexed, followed by centrifugation at 2,750 × g, 4℃ for 5 min before the samples were loaded onto a 96-well plate with a filter. After the addition of 10 μL of analytical and pooled plasma samples and calibration standards to each well, the plates were dried with a nitrogen evaporator and derivatized with phenyl isothiocyanate. Then, dried samples were extracted with ammonium acetate solution in methanol and aliquoted into two deep-well plates for liquid chromatography mode and flow injection analysis (FIA) mode (described in the manual), followed by dilution with water and an FIA solvent, respectively. Both deep-well plates were placed in an autosampler of Ultimate 3000 ultra-performance liquid chromatography coupled with a Q Exactive Plus hybrid quadrupole-orbitrap mass spectrometer (Thermo Fisher Scientific, MA, USA) and analyzed using the validated method. |
| Sampleprep Protocol Filename: | UM-p400-HR-Thermo-3.pdf |
| Processing Storage Conditions: | On ice |
| Extract Storage: | 4℃ |
