Summary of Study ST002174
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001383. The data can be accessed directly via it's Project DOI: 10.21228/M8Z993 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST002174 |
| Study Title | Identifying a tryptophan derivative in hydrogen peroxide-treated cell culture medium |
| Study Summary | Reactive oxygen species (ROS) are by-products of metabolism of oxygen and they play an important role in normal homeostasis and cell signaling, as well as in the initiation of diseases including cancer when their production is upregulated. Thus, it is imperative to understand the cellular and molecular basis by which ROS impact on various biological and pathological processes. Here, we identified 2-oxindole, a tryptophan derivative, was a major catabolic product in hydrogen peroxide-treated cell culture medium. We used 2-oxindole to study its role in regulating AhR signaling and tryptophan metabolic pathways. We found that 2-oxindole significantly increased the activity of AhR, leading to enhanced expression of its downstream targets including cytochrome P450 genes. |
| Institute | NYU Langone Health |
| Department | Department of Biochemistry and Molecular Pharmacology |
| Laboratory | Dai |
| Last Name | Choi |
| First Name | Byeong Hyeok |
| Address | 341 East 25 Street New York, NY 10010 |
| ByeongHyeok.Choi@nyulangone.org | |
| Phone | 2122635521 |
| Submit Date | 2022-04-06 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-06-01 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP002266 |
| Sampleprep Summary: | Samples were extracted with methanol and in house cocktail of amino acids, acting as an internal standards. Samples were then homogenized and spun down, and supernatant was dried down. Dried down samples were reconstituted and transferred to LCMS vials, then injected on LCMS at 2ul per sample |
| Sampleprep Protocol ID: | 0.01.12.12 |
| Processing Storage Conditions: | 4℃ |
| Extraction Method: | E.01 |
| Extract Storage: | -80℃ |
| Sample Resuspension: | 50uL |
| Sample Spiking: | 500nM |
