Summary of Study ST002174

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001383. The data can be accessed directly via it's Project DOI: 10.21228/M8Z993 This work is supported by NIH grant, U2C- DK119886.


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Study IDST002174
Study TitleIdentifying a tryptophan derivative in hydrogen peroxide-treated cell culture medium
Study SummaryReactive oxygen species (ROS) are by-products of metabolism of oxygen and they play an important role in normal homeostasis and cell signaling, as well as in the initiation of diseases including cancer when their production is upregulated. Thus, it is imperative to understand the cellular and molecular basis by which ROS impact on various biological and pathological processes. Here, we identified 2-oxindole, a tryptophan derivative, was a major catabolic product in hydrogen peroxide-treated cell culture medium. We used 2-oxindole to study its role in regulating AhR signaling and tryptophan metabolic pathways. We found that 2-oxindole significantly increased the activity of AhR, leading to enhanced expression of its downstream targets including cytochrome P450 genes.
NYU Langone Health
DepartmentDepartment of Biochemistry and Molecular Pharmacology
Last NameChoi
First NameByeong Hyeok
Address341 East 25 Street New York, NY 10010
Submit Date2022-04-06
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2022-06-01
Release Version1
Byeong Hyeok Choi Byeong Hyeok Choi application/zip

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Sample Preparation:

Sampleprep ID:SP002266
Sampleprep Summary:Samples were extracted with methanol and in house cocktail of amino acids, acting as an internal standards. Samples were then homogenized and spun down, and supernatant was dried down. Dried down samples were reconstituted and transferred to LCMS vials, then injected on LCMS at 2ul per sample
Sampleprep Protocol ID:
Processing Storage Conditions:4℃
Extraction Method:E.01
Extract Storage:-80℃
Sample Resuspension:50uL
Sample Spiking:500nM