Summary of Study ST002126

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001348. The data can be accessed directly via it's Project DOI: 10.21228/M8GD8D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002126
Study Title	Amino acids and TCA substrates in hematopoietic cells (Part3)
Study Summary	This study uses [13C,15N] labeled amino acids to study the amino acid consumption and their catabolism into tricarboxylic acid cycle substrates in hematopoietic stem cells, hemopoietic progenitors, and differentiated hematopoietic cells under different conditions, such as homeostasis and proliferation and with drug treatment.
Institute	Sun Yat-sen University
Last Name	Zhao
First Name	Meng
Address	Zhongshan 2nd Road
Email	zhaom38@mail.sysu.edu.cn
Phone	18138799889
Submit Date	2022-04-06
Raw Data Available	Yes
Raw Data File Type(s)	cdf
Analysis Type Detail	GC-MS
Release Date	2022-11-01
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP002217
Sampleprep Summary:	To get the corresponding derivatives, the dried aliquots were incubated with 20 µL 2% (w/v) methoxyamine hydrochloride (226904, Sigma-Aldrich) in pyridine for 60 min at 37 °C, and silylated by 30 µL of N-Methyl-N-(tert-butyldimethylsilyl) trifluoroacetamide with 1% tert-Butyldimethylchlorosilane (TBDMS, 18162-48-6, Regis Technologies) for 30 min at 45 °C. The corresponding derivatives were analyzed by GC-MS using the Trace 1310 gas chromatograph (Thermo Fisher) with the DB-35ms column (Agilent Technologies) connected to the Q ExactiveTM GC OrbitrapTM GC-MS/MS system (Thermo Fisher).

Sampleprep ID:

SP002217

Sampleprep Summary:

To get the corresponding derivatives, the dried aliquots were incubated with 20 µL 2% (w/v) methoxyamine hydrochloride (226904, Sigma-Aldrich) in pyridine for 60 min at 37 °C, and silylated by 30 µL of N-Methyl-N-(tert-butyldimethylsilyl) trifluoroacetamide with 1% tert-Butyldimethylchlorosilane (TBDMS, 18162-48-6, Regis Technologies) for 30 min at 45 °C. The corresponding derivatives were analyzed by GC-MS using the Trace 1310 gas chromatograph (Thermo Fisher) with the DB-35ms column (Agilent Technologies) connected to the Q ExactiveTM GC OrbitrapTM GC-MS/MS system (Thermo Fisher).