Summary of Study ST001971

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001254. The data can be accessed directly via it's Project DOI: 10.21228/M8MM7B This work is supported by NIH grant, U2C- DK119886.


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Study IDST001971
Study TitleLipid Profiling in African-American Men with Prostate Cancer
Study Summarywe examined the lipidomes from cancer-benign matched prostate tissues in PCa patients of AA and EA men to determine potential alterations in lipid metabolism which might explain the observed disparity in tumor progression.
Baylor College of Medicine
Last NameSreekumar
First NameArun
AddressOne Baylor Plaza
Submit Date2021-10-23
Analysis Type DetailLC-MS
Release Date2022-11-01
Release Version1
Arun Sreekumar Arun Sreekumar application/zip

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Sample Preparation:

Sampleprep ID:SP002057
Sampleprep Summary:We used 50 mg of tissues for sample preparation. Lipids were extracted using a modified Bligh-Dyer method. The extraction was carried out using 2:2:2 volume ratio of water/methanol/dichloromethane at room temperature after spiking internal standards (17:0LPC, 17:0PC, 17:0PE, 17:0PG, 17:0 ceramide, 17:0SM, 17:0PS, 17:0PA, 17:0TAG, 17:0MAG, DAG 16:0/18:1, CE 17:0, and 17.0-20.4 PI). The organic layer was collected and completely dried under nitrogen. Before MS analysis, the dried extract was resuspended in 100 μL of Buffer B (10:5:85 Acetonitrile/water/Isopropyl alcohol) containing 10mM NH4OAc and subjected to LC/MS. The lipidome was separated using reverse-phase chromatography.