Summary of Study ST001971

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001254. The data can be accessed directly via it's Project DOI: 10.21228/M8MM7B This work is supported by NIH grant, U2C- DK119886.

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Study ID	ST001971
Study Title	Lipid Profiling in African-American Men with Prostate Cancer
Study Summary	we examined the lipidomes from cancer-benign matched prostate tissues in PCa patients of AA and EA men to determine potential alterations in lipid metabolism which might explain the observed disparity in tumor progression.
Institute	Baylor College of Medicine
Last Name	Sreekumar
First Name	Arun
Address	One Baylor Plaza
Email	Arun.Sreekumar@bcm.edu
Phone	7137983144
Submit Date	2021-10-23
Analysis Type Detail	LC-MS
Release Date	2022-11-01
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP002057
Sampleprep Summary:	We used 50 mg of tissues for sample preparation. Lipids were extracted using a modified Bligh-Dyer method. The extraction was carried out using 2:2:2 volume ratio of water/methanol/dichloromethane at room temperature after spiking internal standards (17:0LPC, 17:0PC, 17:0PE, 17:0PG, 17:0 ceramide, 17:0SM, 17:0PS, 17:0PA, 17:0TAG, 17:0MAG, DAG 16:0/18:1, CE 17:0, and 17.0-20.4 PI). The organic layer was collected and completely dried under nitrogen. Before MS analysis, the dried extract was resuspended in 100 μL of Buffer B (10:5:85 Acetonitrile/water/Isopropyl alcohol) containing 10mM NH4OAc and subjected to LC/MS. The lipidome was separated using reverse-phase chromatography.

Sampleprep ID:

SP002057

Sampleprep Summary:

We used 50 mg of tissues for sample preparation. Lipids were extracted using a modified Bligh-Dyer method. The extraction was carried out using 2:2:2 volume ratio of water/methanol/dichloromethane at room temperature after spiking internal standards (17:0LPC, 17:0PC, 17:0PE, 17:0PG, 17:0 ceramide, 17:0SM, 17:0PS, 17:0PA, 17:0TAG, 17:0MAG, DAG 16:0/18:1, CE 17:0, and 17.0-20.4 PI). The organic layer was collected and completely dried under nitrogen. Before MS analysis, the dried extract was resuspended in 100 μL of Buffer B (10:5:85 Acetonitrile/water/Isopropyl alcohol) containing 10mM NH4OAc and subjected to LC/MS. The lipidome was separated using reverse-phase chromatography.