Summary of Study ST001960

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001247. The data can be accessed directly via it's Project DOI: 10.21228/M8HT3Z This work is supported by NIH grant, U2C- DK119886.

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Study IDST001960
Study TitleLipidomics of brown adipocytes treated with d9-choline
Study Typetargeted LC-QTOF/MS lipidomic profiling
Study SummaryBrown adipose tissue (BAT) was initially characterised as a thermogenic organ, and recent studies have suggested it plays a crucial role in maintaining systemic metabolic health. In this project, we demonstrated that alteration of BAT function contributes to development of heart failure through disorientation in choline metabolism. To analyze the detail effect of choline accumulation on brown adipocytes, we conducted the LC-QTOF/MS analysis using cultured brown adipocytes treated with d9-choline. In brown adipocytes treated with d9-choline, we found increase of phosphatidylcholine and lysophosphatidylcholine, suggesting that choline was metabolized in healthy brown adipocytes.
Institute
Juntendo University
DepartmentDepartment of Cardiovascular Biology and Medicine
Last NameYoshida
First NameYohko
Address2-1-1, Hongo, Bunkyo-ku, Tokyo, Tokyo, 1138421, Japan
Emailyohko105@yahoo.co.jp
Phone+81-3-3813-3111
Submit Date2021-10-14
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2022-11-01
Release Version1
Yohko Yoshida Yohko Yoshida
https://dx.doi.org/10.21228/M8HT3Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP002046
Sampleprep Summary:Lipids were extracted using a methanol–chloroform–water extraction method. The methanol used contained 25 μM each of methionine sulfone and D-camphor-10-sulphonic acid and 10 μM each of 1,2-dimyristoyl(d54)-sn-glycero-3-phosphocholine and 1,2-dihexanoyl(d22)-sn-glycero-3-phosphocholine.
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