Summary of Study ST001614

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001037. The data can be accessed directly via it's Project DOI: 10.21228/M8NM4H This work is supported by NIH grant, U2C- DK119886.


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Study IDST001614
Study TitleNMR metabolomics analysis of ricin-induced and fasting hypoglycemia (part-I)
Study TypeTargeted NMR
Study SummaryMice were subject to ricin exposure or fasting conditions for 2 hours, 8 hours, or an overnight time period. Following treatment, livers were removed and metabolites were extracted and analyzed by NMR.
Montana State University
DepartmentChemistry & Biochemistry
Last NameKempa
First NameJake
Address103 Chemistry and Biochemistry Building
Submit Date2020-09-23
Num Groups9
Total Subjects107
Num Males54
Num Females53
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailNMR
Release Date2022-11-29
Release Version1
Jake Kempa Jake Kempa application/zip

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Sample Preparation:

Sampleprep ID:SP001697
Sampleprep Summary:Frozen livers were homogenized in liquid nitrogen using a mortar and pestle. Liver powder was weighed (60-80 mg) into 2-mL microcentrifuge tubes. 900 µL of ice-cold 1:2 MeOH/CHCl3 was added to the liver tissue and tissue was mixed into suspension and lysed using a FastPrep-24TM 5G homogenizer using the S. aureus setting of 6.0 meters/second speed for 2 x 40 seconds. Tubes were placed back on ice and 200 µL of ice-cold H2O was added, before repeating the lysis step. Samples were then placed at -20 ºC for approximately 45 minutes to precipitate protein, then centrifuged at 14,000 g for 10 minutes. 250 µL of upper polar phase were transferred to a 1.5 mL Eppendorf tube and dried overnight using a Savant™ Universal SpeedVac™ vacuum system with no heat. Dried metabolite mixtures were re-suspended in 600 µL of NMR buffer containing 0.25 mM 4,4-dimethyl-4-silapentane-1-sulfonic acid (DSS), 90%H2O/10% D2O, 25 mM sodium phosphate, pH 7.