Summary of Study ST001365
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000933. The data can be accessed directly via it's Project DOI: 10.21228/M8398Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001365 |
| Study Title | Antibiotics in Cystic Fibrosis Sputum |
| Study Type | Clinical |
| Study Summary | Detection of 18 antibiotics in 171 cystic fibrosis sputum samples from the Phase 1 Clinical Study. |
| Institute | University of California, Irvine |
| Last Name | Gallagher |
| First Name | Tara |
| Address | 3315 McGaugh Hall, UC IRVINE, Irvine, CA, 92697, USA |
| tgallagh@uci.edu | |
| Phone | 9498243509 |
| Submit Date | 2020-04-23 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Waters) |
| Analysis Type Detail | LC-MS |
| Release Date | 2020-06-01 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP001447 |
| Sampleprep Summary: | The extraction solvent was chilled 1% DTT and spiked with 1.33 µM of both internal standards, linezolid-d3 and levofloxacin-d8. Solvent (150 µL) was added to 50 µL of sputum. The samples were vortexed for 30 seconds, shaken at 4˚C on a shaking platform with moderate agitation for 15 minutes, and centrifuged at 13,200 RCF for 10 minutes at 4˚C. The supernatant was pipetted into amber glass vials and injected directly into the LC-MS. |
| Processing Storage Conditions: | On ice |
| Extract Storage: | On ice |
