Summary of Study ST001365

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000933. The data can be accessed directly via it's Project DOI: 10.21228/M8398Z This work is supported by NIH grant, U2C- DK119886.


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Study IDST001365
Study TitleAntibiotics in Cystic Fibrosis Sputum
Study TypeClinical
Study SummaryDetection of 18 antibiotics in 171 cystic fibrosis sputum samples from the Phase 1 Clinical Study.
University of California, Irvine
Last NameGallagher
First NameTara
Address3315 McGaugh Hall, UC IRVINE, Irvine, CA, 92697, USA
Submit Date2020-04-23
Raw Data AvailableYes
Raw Data File Type(s)raw(Waters)
Analysis Type DetailLC-MS
Release Date2020-06-01
Release Version1
Tara Gallagher Tara Gallagher application/zip

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Sample Preparation:

Sampleprep ID:SP001447
Sampleprep Summary:The extraction solvent was chilled 1% DTT and spiked with 1.33 µM of both internal standards, linezolid-d3 and levofloxacin-d8. Solvent (150 µL) was added to 50 µL of sputum. The samples were vortexed for 30 seconds, shaken at 4˚C on a shaking platform with moderate agitation for 15 minutes, and centrifuged at 13,200 RCF for 10 minutes at 4˚C. The supernatant was pipetted into amber glass vials and injected directly into the LC-MS.
Processing Storage Conditions:On ice
Extract Storage:On ice