Summary of Study ST001231

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000824. The data can be accessed directly via it's Project DOI: 10.21228/M85Q5K This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001231
Study TitlePlasma untargeted metabolomics study of pulmonary tuberculosis
Study SummaryIn this study, differentially abundant plasma metabolites were screened by using the ultra-high performance liquid chromatography coupled with Q Exactive mass spectrometry in pulmonary tuberculosis(TB) patients and normal controls(NC) or patients with other pulmonary diseases such as, community-acquired pneumonia(CAP) and lung cancer(LC).
Zhengjiang University
DepartmentSchool of Medicine
LaboratoryInstitute of Cell Biology
Last NameLi
First NameJi-Cheng
Address866 Yuhangtang Road, West Lake District, Hangzhou, Zhejiang Province, 310058, PR China
Submit Date2019-08-05
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2020-12-01
Release Version1
Ji-Cheng Li Ji-Cheng Li application/zip

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Sample Preparation:

Sampleprep ID:SP001307
Sampleprep Summary:The frozen heparin anti-coagulated plasma samples were thawed on ice and 100 μl of each sample was transferred to a 1.5ml sterile micro-centrifugen tube. After the addition of 300 μl of methanol (containing internal standard L-2-chlorophenylalanine 1 μg/ml), each sample was vortexed for 30 s, sonicated for 10 min in ice-water bath, and incubated for 1 h at -20°C to precipitate proteins. Then each sample was centrifuged at 12000 rpm for 15 min at 4°C. The resulting supernatants were transferred to LC-MS vials and stored at -80°C until the UPLC-QE-MS analysis. Each quality control sample was also prepared by mixing an equal aliquot of the supernatants from each sample. The treatment methods were the same as above.