Summary of Study ST000962

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000660. The data can be accessed directly via it's Project DOI: 10.21228/M8C39R This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000962
Study TitleMetabolomics Involved in Early-Life Single Pulse Antibiotic Exposures - Liver (part I)
Study TypeTimecourse treatment Vs control
Study SummaryThe mice liver samples were extracted and analyzed using broad spectrum GCMS for the identification of compounds distinguishing the groups.
University of North Carolina
DepartmentNIH Eastern Regional Comprehensive Metabolomics Resource Core (RTI RCMRC)
Last NameSumner
First NameSusan
Address500 Laureate Way Kannapolis NC 28081
Submit Date2018-04-13
Num Groups10
Total Subjects86
Num Males30
Num Females56
Analysis Type DetailGC-MS
Release Date2018-08-02
Release Version1
Susan Sumner Susan Sumner application/zip

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Sample Preparation:

Sampleprep ID:SP001008
Sampleprep Summary:Extraction, Solvent Removal, Resuspension, Solvent Removal, Two-step Derivatization and Addition of FAME Markers
Processing Method:Liver tissue were frozen on dry ice and weighed at approximate 100 mg to a MagNa Lyser tube with 10 to 15 ceramic beads inside. The ice-cold homogenization buffer (50% acetonitrile) was added with the mass/volume ratio at 1 mg tissue per 5 µL buffer. The homogenization was carried on at 4,500 rpm for two 30 sec pulses by the MagNA Lyser. The homogenized mixture was centrifuged at 14,000 rpm for 5 min at -4 °C for the supernatant. Aliquots of 100 µL homogenized supernatant of the study sample (equal to 20 mg tissue) were mixed with 1000 µL of cold degassed 3:3:2 ACN:ISP:H20 solution in a 2 mL snap cap tube. Samples are vortex and shaken for 5 min, and then centrifuged at 4 °C for 2 min at 14000 rcf. The supernatant was split into (2)-450 µL fractions, one fraction was archived in -80°C the second fraction was subject t complete sample processing. Transfer supernatant to a new vial. Evaporate supernatant to complete dryness at room temp.
Processing Storage Conditions:On ice
Extraction Method:(step 1) 3:3:2 Acetonitrile:Isopropanol:H2O, (step 2) 1:1 Acetonitrile:H2O
Extract Storage:-80℃
Sample Resuspension:Samples resuspended in 200 µL of 1:1 ACN:H2O. Evaporated to dryness w/speed vac.
Sample Derivatization:Formation of methoximes by adding 10µL of 40mg/mL MeOx in pyridine to each sample. Placed onto Thermomixer for 90 min at 30⁰C. Then FAME retention index markers and MSTFA added to each sample for derivatization step. Samples returned to Thermomixer for 45 min at 70⁰C.
Sample Spiking:Fatty acid methyl esters (FAME)