Summary of Study ST000962
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000660. The data can be accessed directly via it's Project DOI: 10.21228/M8C39R This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000962 |
| Study Title | Metabolomics Involved in Early-Life Single Pulse Antibiotic Exposures - Liver (part I) |
| Study Type | Timecourse treatment Vs control |
| Study Summary | The mice liver samples were extracted and analyzed using broad spectrum GCMS for the identification of compounds distinguishing the groups. |
| Institute | University of North Carolina |
| Department | NIH Eastern Regional Comprehensive Metabolomics Resource Core (RTI RCMRC) |
| Laboratory | UNC NRI ERCMRC GCMS Core |
| Last Name | Sumner |
| First Name | Susan |
| Address | 500 Laureate Way Kannapolis NC 28081 |
| susan_sumner@unc.edu | |
| Phone | 1-919-622-4456 |
| Submit Date | 2018-04-13 |
| Num Groups | 10 |
| Total Subjects | 86 |
| Num Males | 30 |
| Num Females | 56 |
| Analysis Type Detail | GC-MS |
| Release Date | 2018-08-02 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP001008 |
| Sampleprep Summary: | Extraction, Solvent Removal, Resuspension, Solvent Removal, Two-step Derivatization and Addition of FAME Markers |
| Processing Method: | Liver tissue were frozen on dry ice and weighed at approximate 100 mg to a MagNa Lyser tube with 10 to 15 ceramic beads inside. The ice-cold homogenization buffer (50% acetonitrile) was added with the mass/volume ratio at 1 mg tissue per 5 µL buffer. The homogenization was carried on at 4,500 rpm for two 30 sec pulses by the MagNA Lyser. The homogenized mixture was centrifuged at 14,000 rpm for 5 min at -4 °C for the supernatant. Aliquots of 100 µL homogenized supernatant of the study sample (equal to 20 mg tissue) were mixed with 1000 µL of cold degassed 3:3:2 ACN:ISP:H20 solution in a 2 mL snap cap tube. Samples are vortex and shaken for 5 min, and then centrifuged at 4 °C for 2 min at 14000 rcf. The supernatant was split into (2)-450 µL fractions, one fraction was archived in -80°C the second fraction was subject t complete sample processing. Transfer supernatant to a new vial. Evaporate supernatant to complete dryness at room temp. |
| Processing Storage Conditions: | On ice |
| Extraction Method: | (step 1) 3:3:2 Acetonitrile:Isopropanol:H2O, (step 2) 1:1 Acetonitrile:H2O |
| Extract Storage: | -80℃ |
| Sample Resuspension: | Samples resuspended in 200 µL of 1:1 ACN:H2O. Evaporated to dryness w/speed vac. |
| Sample Derivatization: | Formation of methoximes by adding 10µL of 40mg/mL MeOx in pyridine to each sample. Placed onto Thermomixer for 90 min at 30⁰C. Then FAME retention index markers and MSTFA added to each sample for derivatization step. Samples returned to Thermomixer for 45 min at 70⁰C. |
| Sample Spiking: | Fatty acid methyl esters (FAME) |
