Summary of Study ST000266

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000187. The data can be accessed directly via it's Project DOI: 10.21228/M8388M This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Show all samples  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files
Study IDST000266
Study TitleActivity-Based Metabolic Profiling of M. tuberculosis H37Rv Rv3801
Study Typetimecourse
Study SummaryThese experiments involve incubating various proteins of unknown function in the M. tuberculosis genome with whole-cell lysate and assaying changes in metabolite levels over time (e.g. 0, 15, 30min) to try to infer what reaction they might catalyze.
Institute
Weill Cornell Medicine
DepartmentMicrobiology and Immunology
Last NameRhee
First NameKyu
AddressDepartment of Microbiology and Immunology, Weill Cornell Medical College, 1300 York Avenue, New York, NY
Emailkyr9001@med.cornell.edu
Submit Date2015-11-13
Num Groups3 replicates X 3 timepoints
Study CommentsThe timepoints in the datafiles refer the amount of time (in minutes) that each protein was incubated with lysate.The units for 'mzmed' are mass/charge.
The units for 'rtmed' are seconds (retention time).
The units for all the other data columns in the spreadsheets are arbitrary; they are integrated peak counts.
In this study, Rv1713 was incubated with lysate with and without 1mM GTP added as a co-factor.
Raw Data AvailableNo
Analysis Type DetailLC-MS
Release Date2016-12-22
Release Version2
Release CommentsUpdated study design factors
Kyu Rhee Kyu Rhee
https://dx.doi.org/10.21228/M8388M
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Sample Preparation:

Sampleprep ID:SP000294
Sampleprep Summary:10 uM enzyme incubated at 37C in M. bovis whole-cell extract
Processing Method:extract was lyophilized and resuspended in 20 mM Tris-HCL pH 8.0
Processing Storage Conditions:cell pellet frozen at -80C
Extraction Method:70% acetonitrile
Extract Cleanup:centrifugation followed by aspiration of supernatant and lyophilization
Extract Storage:frozen at -80C
  logo