Summary of Study ST000263
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000187. The data can be accessed directly via it's Project DOI: 10.21228/M8388M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000263 |
Study Title | Activity-Based Metabolic Profiling of M. tuberculosis H37Rv Rv1016 |
Study Type | timecourse |
Study Summary | These experiments involve incubating various proteins of unknown function in the M. tuberculosis genome with whole-cell lysate and assaying changes in metabolite levels over time (e.g. 0, 15, 30min) to try to infer what reaction they might catalyze. |
Institute | Weill Cornell Medicine |
Department | Microbiology and Immunology |
Last Name | Rhee |
First Name | Kyu |
Address | Department of Microbiology and Immunology, Weill Cornell Medical College, 1300 York Avenue, New York, NY |
kyr9001@med.cornell.edu | |
Submit Date | 2015-11-13 |
Num Groups | 3 replicates X 3 timepoints |
Study Comments | The timepoints in the datafiles refer the amount of time (in minutes) that each protein was incubated with lysate.The units for 'mzmed' are mass/charge. The units for 'rtmed' are seconds (retention time). The units for all the other data columns in the spreadsheets are arbitrary; they are integrated peak counts. In this study, Rv1713 was incubated with lysate with and without 1mM GTP added as a co-factor. |
Raw Data Available | No |
Raw Data File Type(s) | d |
Analysis Type Detail | LC-MS |
Release Date | 2016-12-22 |
Release Version | 2 |
Release Comments | Updated study design factors |
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Sample Preparation:
Sampleprep ID: | SP000291 |
Sampleprep Summary: | 10 uM enzyme incubated at 25C in M. bovis whole-cell extract |
Processing Method: | extract was lyophilized and resuspended in 20 mM Tris-HCL pH 8.0 |
Processing Storage Conditions: | cell pellet frozen at -80C |
Extraction Method: | 70% acetonitrile |
Extract Cleanup: | centrifugation followed by aspiration of supernatant and lyophilization |
Extract Storage: | frozen at -80C |