Summary of Study ST004274
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002700. The data can be accessed directly via it's Project DOI: 10.21228/M8NK11 This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST004274 |
| Study Title | Loss of vitamin C biosynthesis protects from the pathology of a Schistosome infection and egg production. |
| Study Type | Treatment experiment |
| Study Summary | The processes influenced by ascorbate treatment in schistosome reproduction were investigated by metabolomics analysis of Ascorbate-treated and -untreated worm samples in the culture media. The top metabolite enriched in ascorbate-treated schistosomes was L-DOPA, a product of tyrosinase activity, which crosslinks vitellocyte proteins to form the schistosome eggshell, consistent with a role for ascorbate in vitellocyte development. |
| Institute | University of Texas Southwestern Medical Center at Dallas |
| Department | Children’s Medical Center Research Institute |
| Laboratory | Michalis Agathokleous |
| Last Name | Agathokleous |
| First Name | Michalis |
| Address | 6000 Harry Hines Blvd, NL12.110N, Dallas, TX, 75235 |
| michail.agathokleous@utsouthwestern.edu | |
| Phone | 2146486270 |
| Submit Date | 2025-10-06 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2025-10-31 |
| Release Version | 1 |
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Project:
| Project ID: | PR002700 |
| Project DOI: | doi: 10.21228/M8NK11 |
| Project Title: | Loss of vitamin C biosynthesis protects from the pathology of a Schistosome infection |
| Project Type: | MS quantitative analysis |
| Project Summary: | Schistosoma parasite is known to require ascorbate to produce eggs in vitro culture. To investigate the effect of ascorbate deficiency in mice infected with Schistosoma mansoni, the pathology of infection was analyzed in vivo. S. mansoni required host ascorbate to produce eggs and consequently, ascorbate-deficient mice were protected from schistosomiasis pathologies including liver granuloma formation and transmission. To understand the processes dependent on ascorbate metabolome and gene expression profiles were compared between Ascorbate-treated and -untreated samples. Our work shows that ascorbate deficiency can have physiological benefits by protecting animals from the pathology of a major parasitic disease. |
| Institute: | UT Southwestern Medical Center |
| Department: | CRI |
| Laboratory: | Michalis Agathokleous |
| Last Name: | Agathokleous |
| First Name: | Michalis |
| Address: | 5323 Harry Hines Blvd, Children's Research Institute, Dallas, TX, 75309, USA |
| Email: | michail.agathokleous@utsouthwestern.edu |
| Phone: | 2146486270 |
