Summary of Study ST002330
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001494. The data can be accessed directly via it's Project DOI: 10.21228/M8MD91 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002330 |
| Study Title | Early-stage responses to Plasmodiophora brassicae at the metabolome levels in clubroot resistant and susceptible oilseed Brassica napus |
| Study Type | Timecourse experiment |
| Study Summary | A total of 36 samples comprised two types of genotypes [CR (5 individuals pooled in each biological replicate) and CS (5 individuals pooled in each biological replicate)], two treatments (inoculated and uninoculated) and three biological replicates generated from three independent experiments and collected at 1-, 4-, and 7–DPI were used to extract primary and secondary metabolites and analyse the differences among the treatments. |
| Institute | Trent University |
| Department | Biology |
| Laboratory | Emery Lab |
| Last Name | Kisiala |
| First Name | Anna |
| Address | 1600 West Bank Drive, Trent University |
| annakisiala@trentu.ca | |
| Phone | 7057481011 |
| Submit Date | 2022-10-17 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-11-16 |
| Release Version | 1 |
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Project:
| Project ID: | PR001494 |
| Project DOI: | doi: 10.21228/M8MD91 |
| Project Title: | Early-stage responses to Plasmodiophora brassicae at the metabolome levels in clubroot resistant and susceptible oilseed Brassica napus |
| Project Type: | MS quantitative analysis |
| Project Summary: | Clubroot, a devastating soil-borne root disease, in Brassicaceae is caused by Plasmodiophora brassicae Woronin, an obligate biotrophic protist. Plant growth and development, as well as seed yield of Brassica crops, are severely affected due to this disease. Several reports described the molecular responses of B. napus to P. brassicae; however, information on the early stages of pathogenesis is limited. In this study, we have used metabolomics approach to characterize P. brassicae pathogenesis at 1-, 4-, and 7- days post-inoculation (DPI) in clubroot resistant (CR) and susceptible (CS) doubled-haploid (DH) lines. Several metabolites related to organic acids (e.g., citrate, pyruvate), amino acids (e.g., proline, aspartate), sugars, and mannitol, were differentially accumulated in roots in response to pathogen infection when the CR and CS genotypes were compared. Our results suggest important roles for these metabolites in mediating resistance to clubroot disease. To our knowledge, this is the first report of an integrated metabolome analysis aimed at characterizing the molecular basis of resistance to clubroot in canola. |
| Institute: | Trent University |
| Department: | Biology |
| Laboratory: | Emery Lab |
| Last Name: | Kisiala |
| First Name: | Anna |
| Address: | 1600 West Bank Drive, Trent University, Peterborough, ON, K9L 0G2, Canada |
| Email: | annakisiala@trentu.ca |
| Phone: | 7057481011 |
| Funding Source: | Alberta Agriculture and Forestry |
| Contributors: | Dinesh Adhikary, Anna Kisiala, Ananya Sarkar, Urmila Basu, Habibur Rahman, Neil Emery, Nat N. V. Kav |
