Summary of Study ST002270
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001452. The data can be accessed directly via it's Project DOI: 10.21228/M81Q5B This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST002270 |
| Study Title | Xenopus tropicalis glycolysis and PPP inhibition |
| Study Summary | Stage 41 tadpoles were injected with 4 nmol of the glycolysis inhibitor, 2-deoxyglucose (2DG), or a tracer control to evaluate consequences of inhibition on metabolites 24 hours after treatment began. Similarly, tadpoles were incubated in DMSO control or 1 of 2 G6PD inhibitors (Dehydroepiandrosterone and g6pdi), to similarly assess the consequences of inhibiting the pentose phosphate pathway. We find that inhibition of glucose metabolism with 2DG results in a decrease in downstream glycolytic intermediates, confirming a reduction in activity of this pathway. G6PD inhibition was not as clear as changes were less consistent across treatments and downstream metabolites did not behave in a coordinated way, though impacts on other metabolic processes by these inhibitors may be fruitful for exploring how they perturb metabolism in the tadpoles. |
| Institute | University of Washington |
| Last Name | Patel |
| First Name | Jeet |
| Address | 1705 NE Pacific St., HSB J-Wing, J405, Seattle, Washington, 98195, USA |
| pateljeet1224@gmail.com | |
| Phone | 2065431748 |
| Submit Date | 2022-08-03 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-10-25 |
| Release Version | 1 |
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Project:
| Project ID: | PR001452 |
| Project DOI: | doi: 10.21228/M81Q5B |
| Project Title: | Glucose metabolism during Xenopus Regeneration |
| Project Summary: | Targeted metabolomics of Xenopus tropicalis to study glucose metabolism during regeneration |
| Institute: | University of Washington |
| Department: | Biochemistry |
| Laboratory: | Wills Lab |
| Last Name: | Patel |
| First Name: | Jeet |
| Address: | 1705 NE Pacific St., HSB J-Wing, J405, Seattle, Washington, 98195, USA |
| Email: | pateljeet1224@gmail.com |
| Phone: | 2065431748 |
| Funding Source: | Royalty Research Fund, 1R01NS099124 from NINDS |
